Influence of formulation of ZnO nanoblokes containing metallic ions dopants on their cytotoxicity and protective factors: An in vitro study on human skin cells exposed to UVA radiation
Application of ZnO nanoparticles in sunscreens exposes human skin with their adverse effects, which correlates to dissolution/translocation of free Zn+2 ions. The possibility of decreasing solubility and therefore, reducing toxicity, by structural modifications have been discussed as a solution. The...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Toxicology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750018301355 |
| _version_ | 1818700745222914048 |
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| author | Parvaneh Ghaderi-Shekhi Abadi Farshad H. Shirazi Mohammad Joshaghani Hamid R. Moghimi |
| author_facet | Parvaneh Ghaderi-Shekhi Abadi Farshad H. Shirazi Mohammad Joshaghani Hamid R. Moghimi |
| author_sort | Parvaneh Ghaderi-Shekhi Abadi |
| collection | DOAJ |
| description | Application of ZnO nanoparticles in sunscreens exposes human skin with their adverse effects, which correlates to dissolution/translocation of free Zn+2 ions. The possibility of decreasing solubility and therefore, reducing toxicity, by structural modifications have been discussed as a solution. The present investigation has developed new metallic lattices of ZnO to reduce cytotoxicity of ZnO nanoparticles. Novel metal-promoted Zn-based nanocomposites ([Zn(O)/M], M = Mg, Al, Ca, Ti) were synthesized and their physicochemical properties and their cytotoxicity were evaluated. Solubility and release studies showed that modification of ZnO structure decreases release of Zn+2 into culture medium. XRD and UV absorbance analyses showed that metallic-dopants percolate into crystalline lattice of ZnO. This phenomenon is basic reason for stability of Zn-based network. Cell culture studies and MTT assay on human skin cells (HFF-1) exposed to UVA radiation showed that the level of protection of [Zn(O)/M] compounds were more than of [ZnO]. Dichlorofluoroscein diacetate-ROS assay and Zn+2 release experiments indicated that [Zn(O)/M] nanocomposites decreased the level of ROS generation and Zn+2 release in compared to ZnO, indicating higher safety of nanocomposites. This study shows that the synthesized Zn-based nanocomposites have potential to be used as safer and more effective sunscreens than ZnO. Keywords: Metal-promoted Zn-based nanocomposite, Safe ZnO nanoparticles, Human skin cells, UVA radiation, Cytotoxicity and protective effects |
| first_indexed | 2024-12-17T15:09:49Z |
| format | Article |
| id | doaj.art-c783693298d642c88f51cce7d5c8379a |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-12-17T15:09:49Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-c783693298d642c88f51cce7d5c8379a2022-12-21T21:43:42ZengElsevierToxicology Reports2214-75002018-01-015468479Influence of formulation of ZnO nanoblokes containing metallic ions dopants on their cytotoxicity and protective factors: An in vitro study on human skin cells exposed to UVA radiationParvaneh Ghaderi-Shekhi Abadi0Farshad H. Shirazi1Mohammad Joshaghani2Hamid R. Moghimi3Department of Pharmaceutics and Nanotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Institute of Nanoscience and Nanotechnology, Razi University, Kermanshah, IranPharmacology and Toxicolgoy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, IranInstitute of Nanoscience and Nanotechnology, Razi University, Kermanshah, Iran; Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, IranDepartment of Pharmaceutics and Nanotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Protein Technology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Corresponding author at: School of Pharmacy, Shahid Beheshti University of Medical Sciences, Valiasr Ave., Niayesh Junction, PO Box 14155-6153, Tehran, Iran.Application of ZnO nanoparticles in sunscreens exposes human skin with their adverse effects, which correlates to dissolution/translocation of free Zn+2 ions. The possibility of decreasing solubility and therefore, reducing toxicity, by structural modifications have been discussed as a solution. The present investigation has developed new metallic lattices of ZnO to reduce cytotoxicity of ZnO nanoparticles. Novel metal-promoted Zn-based nanocomposites ([Zn(O)/M], M = Mg, Al, Ca, Ti) were synthesized and their physicochemical properties and their cytotoxicity were evaluated. Solubility and release studies showed that modification of ZnO structure decreases release of Zn+2 into culture medium. XRD and UV absorbance analyses showed that metallic-dopants percolate into crystalline lattice of ZnO. This phenomenon is basic reason for stability of Zn-based network. Cell culture studies and MTT assay on human skin cells (HFF-1) exposed to UVA radiation showed that the level of protection of [Zn(O)/M] compounds were more than of [ZnO]. Dichlorofluoroscein diacetate-ROS assay and Zn+2 release experiments indicated that [Zn(O)/M] nanocomposites decreased the level of ROS generation and Zn+2 release in compared to ZnO, indicating higher safety of nanocomposites. This study shows that the synthesized Zn-based nanocomposites have potential to be used as safer and more effective sunscreens than ZnO. Keywords: Metal-promoted Zn-based nanocomposite, Safe ZnO nanoparticles, Human skin cells, UVA radiation, Cytotoxicity and protective effectshttp://www.sciencedirect.com/science/article/pii/S2214750018301355 |
| spellingShingle | Parvaneh Ghaderi-Shekhi Abadi Farshad H. Shirazi Mohammad Joshaghani Hamid R. Moghimi Influence of formulation of ZnO nanoblokes containing metallic ions dopants on their cytotoxicity and protective factors: An in vitro study on human skin cells exposed to UVA radiation Toxicology Reports |
| title | Influence of formulation of ZnO nanoblokes containing metallic ions dopants on their cytotoxicity and protective factors: An in vitro study on human skin cells exposed to UVA radiation |
| title_full | Influence of formulation of ZnO nanoblokes containing metallic ions dopants on their cytotoxicity and protective factors: An in vitro study on human skin cells exposed to UVA radiation |
| title_fullStr | Influence of formulation of ZnO nanoblokes containing metallic ions dopants on their cytotoxicity and protective factors: An in vitro study on human skin cells exposed to UVA radiation |
| title_full_unstemmed | Influence of formulation of ZnO nanoblokes containing metallic ions dopants on their cytotoxicity and protective factors: An in vitro study on human skin cells exposed to UVA radiation |
| title_short | Influence of formulation of ZnO nanoblokes containing metallic ions dopants on their cytotoxicity and protective factors: An in vitro study on human skin cells exposed to UVA radiation |
| title_sort | influence of formulation of zno nanoblokes containing metallic ions dopants on their cytotoxicity and protective factors an in vitro study on human skin cells exposed to uva radiation |
| url | http://www.sciencedirect.com/science/article/pii/S2214750018301355 |
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