The Antinociceptive Properties of the Corydalis yanhusuo Extract.
Corydalis yanhusuo. W.T. extracts (YHS) are widely used for the treatment of pain and inflammation. There are a few studies that assessed the effects of YHS in pain assays; however, none of these studies has systematically compared its activities in the different pain animal modes namely: acute, inf...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5021270?pdf=render |
| _version_ | 1828915437716373504 |
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| author | Lien Wang Yan Zhang Zhiwei Wang Nian Gong Tae Dong Kweon Benjamin Vo Chaoran Wang Xiuli Zhang Jae Yoon Chung Amal Alachkar Xinmiao Liang David Z Luo Olivier Civelli |
| author_facet | Lien Wang Yan Zhang Zhiwei Wang Nian Gong Tae Dong Kweon Benjamin Vo Chaoran Wang Xiuli Zhang Jae Yoon Chung Amal Alachkar Xinmiao Liang David Z Luo Olivier Civelli |
| author_sort | Lien Wang |
| collection | DOAJ |
| description | Corydalis yanhusuo. W.T. extracts (YHS) are widely used for the treatment of pain and inflammation. There are a few studies that assessed the effects of YHS in pain assays; however, none of these studies has systematically compared its activities in the different pain animal modes namely: acute, inflammatory and chronic pain. Furthermore, little is known about the mechanism of YHS activity in these assays. The aim of this study was to systematically evaluate the antinociceptive properties of YHS by testing it in four standardized pain assays and to investigate its mechanism. YHS antinociceptive properties were analyzed in the tail flick, the formalin paw licking, the von Frey filament and the hot box assays after spinal nerve ligation, which monitors acute nociceptive, persistent inflammatory and chronic neuropathic pain, respectively. YHS pharmacological profile was determined by screening it against a battery of G-protein coupled receptors and its mechanism of action was studied using knock-out mice. Our study shows that YHS, at a non-sedative dose, increases the tail flick latency in the tail flick assay without resulting in development of tolerance. YHS also decreases paw licking time in the formalin assay. Further, YHS increases paw withdraw threshold and latency in the von Frey filament and the hot box assays, respectively. In vitro, YHS exhibits prominent dopamine receptor antagonistic properties. In dopamine D2 receptor knockout mice, its antinociceptive effects are attenuated in acute and neuropathic pain but not inflammatory pain assays. Our results therefore indicate that YHS effectively attenuates acute, inflammatory and neuropathic pain, without causing tolerance. The effects on acute and neuropathic pain, but not inflammatory pain, are at least partially mediated through dopamine D2 receptor antagonism. Since YHS is a dietary supplement commercially available in the United States, our data suggest that it might be a candidate for alternative pain treatment. |
| first_indexed | 2024-12-13T20:14:11Z |
| format | Article |
| id | doaj.art-c784ac4ab399474ca8a7151e740bb972 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T20:14:11Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c784ac4ab399474ca8a7151e740bb9722022-12-21T23:32:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01119e016287510.1371/journal.pone.0162875The Antinociceptive Properties of the Corydalis yanhusuo Extract.Lien WangYan ZhangZhiwei WangNian GongTae Dong KweonBenjamin VoChaoran WangXiuli ZhangJae Yoon ChungAmal AlachkarXinmiao LiangDavid Z LuoOlivier CivelliCorydalis yanhusuo. W.T. extracts (YHS) are widely used for the treatment of pain and inflammation. There are a few studies that assessed the effects of YHS in pain assays; however, none of these studies has systematically compared its activities in the different pain animal modes namely: acute, inflammatory and chronic pain. Furthermore, little is known about the mechanism of YHS activity in these assays. The aim of this study was to systematically evaluate the antinociceptive properties of YHS by testing it in four standardized pain assays and to investigate its mechanism. YHS antinociceptive properties were analyzed in the tail flick, the formalin paw licking, the von Frey filament and the hot box assays after spinal nerve ligation, which monitors acute nociceptive, persistent inflammatory and chronic neuropathic pain, respectively. YHS pharmacological profile was determined by screening it against a battery of G-protein coupled receptors and its mechanism of action was studied using knock-out mice. Our study shows that YHS, at a non-sedative dose, increases the tail flick latency in the tail flick assay without resulting in development of tolerance. YHS also decreases paw licking time in the formalin assay. Further, YHS increases paw withdraw threshold and latency in the von Frey filament and the hot box assays, respectively. In vitro, YHS exhibits prominent dopamine receptor antagonistic properties. In dopamine D2 receptor knockout mice, its antinociceptive effects are attenuated in acute and neuropathic pain but not inflammatory pain assays. Our results therefore indicate that YHS effectively attenuates acute, inflammatory and neuropathic pain, without causing tolerance. The effects on acute and neuropathic pain, but not inflammatory pain, are at least partially mediated through dopamine D2 receptor antagonism. Since YHS is a dietary supplement commercially available in the United States, our data suggest that it might be a candidate for alternative pain treatment.http://europepmc.org/articles/PMC5021270?pdf=render |
| spellingShingle | Lien Wang Yan Zhang Zhiwei Wang Nian Gong Tae Dong Kweon Benjamin Vo Chaoran Wang Xiuli Zhang Jae Yoon Chung Amal Alachkar Xinmiao Liang David Z Luo Olivier Civelli The Antinociceptive Properties of the Corydalis yanhusuo Extract. PLoS ONE |
| title | The Antinociceptive Properties of the Corydalis yanhusuo Extract. |
| title_full | The Antinociceptive Properties of the Corydalis yanhusuo Extract. |
| title_fullStr | The Antinociceptive Properties of the Corydalis yanhusuo Extract. |
| title_full_unstemmed | The Antinociceptive Properties of the Corydalis yanhusuo Extract. |
| title_short | The Antinociceptive Properties of the Corydalis yanhusuo Extract. |
| title_sort | antinociceptive properties of the corydalis yanhusuo extract |
| url | http://europepmc.org/articles/PMC5021270?pdf=render |
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