Isolation and Identification of Arsenic Hyper-Tolerant Bacterium with Potential Plant Growth Promoting Properties from Soil
The soil and groundwater of the Bhagobangola I block of Murshidabad district, West Bengal, India is severely arsenic-contaminated. A bacterium was isolated from the garden soil of the Mahishasthali village, which could tolerate 36.49 mM arsenic (III), 280.44 mM arsenic (V) and 63 mM chromium (III),...
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MDPI AG
2022-11-01
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| Series: | Minerals |
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| Online Access: | https://www.mdpi.com/2075-163X/12/11/1452 |
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| author | Debjani Mandal Mina Aghababaei Sadhan Kr Das Santanu Majumder Debashis Chatterjee Abhishek Basu |
| author_facet | Debjani Mandal Mina Aghababaei Sadhan Kr Das Santanu Majumder Debashis Chatterjee Abhishek Basu |
| author_sort | Debjani Mandal |
| collection | DOAJ |
| description | The soil and groundwater of the Bhagobangola I block of Murshidabad district, West Bengal, India is severely arsenic-contaminated. A bacterium was isolated from the garden soil of the Mahishasthali village, which could tolerate 36.49 mM arsenic (III), 280.44 mM arsenic (V) and 63 mM chromium (III), which makes it arsenic (III and V) and chromium (III) hyper-tolerant bacterium. The growth pattern of this bacterium does not show much alteration in the presence of 10 mM arsenic (III) and chromium (III), emphasizing its resistance to these heavy metals. Scanning electron microscopic analysis depicted this bacterium to be rod-shaped with a size of ~1.45 µm. 16S rDNA sequencing, followed by subsequent phylogenetic analysis, established the identity of this bacterium as <i>Microbacterium paraoxydans.</i> This bacterium is capable of bioremediation of arsenic and showed 30.8% and 35.2% of bioremediation for 1mM and 22.6%, and 30.5% of bioremediation for 4mM arsenite, over a period of 24 and 48 h, respectively. <i>Microbacterium paraoxydans</i> also exhibits potential plant growth-promoting properties such as nitrogen fixation, phosphate solubilization, indole-3-acetic acid production and production of siderophores. Therefore, the heavy metal resistance, bioremediation potential and plant growth-promoting potential of the bacterium could be utilized not only for reduction in arsenic toxicity in soil and groundwater but also for plant growth promotion. |
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| institution | Directory Open Access Journal |
| issn | 2075-163X |
| language | English |
| last_indexed | 2024-03-09T18:08:18Z |
| publishDate | 2022-11-01 |
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| series | Minerals |
| spelling | doaj.art-c785cda254134a338dce4b6f7fcf659c2023-11-24T09:18:33ZengMDPI AGMinerals2075-163X2022-11-011211145210.3390/min12111452Isolation and Identification of Arsenic Hyper-Tolerant Bacterium with Potential Plant Growth Promoting Properties from SoilDebjani Mandal0Mina Aghababaei1Sadhan Kr Das2Santanu Majumder3Debashis Chatterjee4Abhishek Basu5Department of Molecular Biology and Biotechnology, Sripat Singh College, Murshidabad 742123, IndiaDepartment of Civil and Environmental Engineering, University of New Hampshire, Durham, NH 03824, USAVivekananda Institute of Biotechnology, Sri Ramkrishna Ashram, Nimpith 743338, IndiaSchool of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKDepartment of Chemistry, University of Kalyani, Nadia 741235, IndiaDepartment of Molecular Biology and Biotechnology, Sripat Singh College, Murshidabad 742123, IndiaThe soil and groundwater of the Bhagobangola I block of Murshidabad district, West Bengal, India is severely arsenic-contaminated. A bacterium was isolated from the garden soil of the Mahishasthali village, which could tolerate 36.49 mM arsenic (III), 280.44 mM arsenic (V) and 63 mM chromium (III), which makes it arsenic (III and V) and chromium (III) hyper-tolerant bacterium. The growth pattern of this bacterium does not show much alteration in the presence of 10 mM arsenic (III) and chromium (III), emphasizing its resistance to these heavy metals. Scanning electron microscopic analysis depicted this bacterium to be rod-shaped with a size of ~1.45 µm. 16S rDNA sequencing, followed by subsequent phylogenetic analysis, established the identity of this bacterium as <i>Microbacterium paraoxydans.</i> This bacterium is capable of bioremediation of arsenic and showed 30.8% and 35.2% of bioremediation for 1mM and 22.6%, and 30.5% of bioremediation for 4mM arsenite, over a period of 24 and 48 h, respectively. <i>Microbacterium paraoxydans</i> also exhibits potential plant growth-promoting properties such as nitrogen fixation, phosphate solubilization, indole-3-acetic acid production and production of siderophores. Therefore, the heavy metal resistance, bioremediation potential and plant growth-promoting potential of the bacterium could be utilized not only for reduction in arsenic toxicity in soil and groundwater but also for plant growth promotion.https://www.mdpi.com/2075-163X/12/11/1452arsenic hyper-tolerant bacterium16S rDNA sequencing<i>Microbacterium paraoxydans</i>arsenic bioremediationplant growth-promoting bacterium |
| spellingShingle | Debjani Mandal Mina Aghababaei Sadhan Kr Das Santanu Majumder Debashis Chatterjee Abhishek Basu Isolation and Identification of Arsenic Hyper-Tolerant Bacterium with Potential Plant Growth Promoting Properties from Soil Minerals arsenic hyper-tolerant bacterium 16S rDNA sequencing <i>Microbacterium paraoxydans</i> arsenic bioremediation plant growth-promoting bacterium |
| title | Isolation and Identification of Arsenic Hyper-Tolerant Bacterium with Potential Plant Growth Promoting Properties from Soil |
| title_full | Isolation and Identification of Arsenic Hyper-Tolerant Bacterium with Potential Plant Growth Promoting Properties from Soil |
| title_fullStr | Isolation and Identification of Arsenic Hyper-Tolerant Bacterium with Potential Plant Growth Promoting Properties from Soil |
| title_full_unstemmed | Isolation and Identification of Arsenic Hyper-Tolerant Bacterium with Potential Plant Growth Promoting Properties from Soil |
| title_short | Isolation and Identification of Arsenic Hyper-Tolerant Bacterium with Potential Plant Growth Promoting Properties from Soil |
| title_sort | isolation and identification of arsenic hyper tolerant bacterium with potential plant growth promoting properties from soil |
| topic | arsenic hyper-tolerant bacterium 16S rDNA sequencing <i>Microbacterium paraoxydans</i> arsenic bioremediation plant growth-promoting bacterium |
| url | https://www.mdpi.com/2075-163X/12/11/1452 |
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