The Effectiveness of Co-Inoculation by Consortia of Microorganisms Depends on the Type of Plant and the Soil Microbiome
The amalgamation of mineral and targeted bacterial preparations represents a new generation of agricultural technology. Inoculation with combined preparations of microorganisms is more effective than inoculation with a single microorganism in stimulating plant growth by providing a more balanced die...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/1/116 |
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| author | Ekaterina Alexeevna Sokolova Olga Viktorovna Mishukova Inna Viktorovna Hlistun Irina Nikolaevna Tromenschleger Artem Yurievich Tikunov Andrey Dmitrievich Manakhov Evgeny Ivanovich Rogaev Oleg Alexandrovich Savenkov Maria Dmitrievna Buyanova Ilya Vladimirovich Ivanov Natalya Valentinovna Smirnova Elena Nikolaevna Voronina |
| author_facet | Ekaterina Alexeevna Sokolova Olga Viktorovna Mishukova Inna Viktorovna Hlistun Irina Nikolaevna Tromenschleger Artem Yurievich Tikunov Andrey Dmitrievich Manakhov Evgeny Ivanovich Rogaev Oleg Alexandrovich Savenkov Maria Dmitrievna Buyanova Ilya Vladimirovich Ivanov Natalya Valentinovna Smirnova Elena Nikolaevna Voronina |
| author_sort | Ekaterina Alexeevna Sokolova |
| collection | DOAJ |
| description | The amalgamation of mineral and targeted bacterial preparations represents a new generation of agricultural technology. Inoculation with combined preparations of microorganisms is more effective than inoculation with a single microorganism in stimulating plant growth by providing a more balanced diet for various crops. In this work, the effect of inoculation of 20 consortium variants on the yield indicators of three crops (wheat, buckwheat, corn) and the soil microbiome in the open field was investigated. The soil microbiome was defined by 16S rRNA sequences through NGS. The species richness of the soil microbial community (alpha diversity) was similar for all studied samples. A beta-diversity analysis revealed that the microbial diversity of three soil samples (C.bw, F.bw and Soil.bw) differed significantly from all others. At the phylum level, the number of <i>Acidobacteriota</i> and <i>Firmicutes</i> in these samples was increased. For the combination “Consortium C (<i>Rothia endophytic GMG9</i> and <i>Azotobacter chroococcum GMG39</i>)—buckwheat”, a systemic positive improvement in all growth and yield indicators was observed. The soil of the site where buckwheat grew, inoculated by Consortium C, contained significantly more available phosphorus than all other soil samples. Such results can be explained both by the direct action of a consortium of phosphate-immobilizing and nitrogen-fixing bacteria and acidification of the medium due to an increase in phylum <i>Acidobacteriota</i> bacteria in the soil. |
| first_indexed | 2024-03-08T14:59:42Z |
| format | Article |
| id | doaj.art-c78a26a741ad404ab4fb123a0e8d71aa |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T14:59:42Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-c78a26a741ad404ab4fb123a0e8d71aa2024-01-10T15:06:21ZengMDPI AGPlants2223-77472023-12-0113111610.3390/plants13010116The Effectiveness of Co-Inoculation by Consortia of Microorganisms Depends on the Type of Plant and the Soil MicrobiomeEkaterina Alexeevna Sokolova0Olga Viktorovna Mishukova1Inna Viktorovna Hlistun2Irina Nikolaevna Tromenschleger3Artem Yurievich Tikunov4Andrey Dmitrievich Manakhov5Evgeny Ivanovich Rogaev6Oleg Alexandrovich Savenkov7Maria Dmitrievna Buyanova8Ilya Vladimirovich Ivanov9Natalya Valentinovna Smirnova10Elena Nikolaevna Voronina11Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaDepartment of Genetics, Centre for Genetics and Life Science, Sirius University of Science and Technology, 354340 Sirius, RussiaDepartment of Genetics, Centre for Genetics and Life Science, Sirius University of Science and Technology, 354340 Sirius, RussiaInstitute of Soil Science and Agrochemistry, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Soil Science and Agrochemistry, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Soil Science and Agrochemistry, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Soil Science and Agrochemistry, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaThe amalgamation of mineral and targeted bacterial preparations represents a new generation of agricultural technology. Inoculation with combined preparations of microorganisms is more effective than inoculation with a single microorganism in stimulating plant growth by providing a more balanced diet for various crops. In this work, the effect of inoculation of 20 consortium variants on the yield indicators of three crops (wheat, buckwheat, corn) and the soil microbiome in the open field was investigated. The soil microbiome was defined by 16S rRNA sequences through NGS. The species richness of the soil microbial community (alpha diversity) was similar for all studied samples. A beta-diversity analysis revealed that the microbial diversity of three soil samples (C.bw, F.bw and Soil.bw) differed significantly from all others. At the phylum level, the number of <i>Acidobacteriota</i> and <i>Firmicutes</i> in these samples was increased. For the combination “Consortium C (<i>Rothia endophytic GMG9</i> and <i>Azotobacter chroococcum GMG39</i>)—buckwheat”, a systemic positive improvement in all growth and yield indicators was observed. The soil of the site where buckwheat grew, inoculated by Consortium C, contained significantly more available phosphorus than all other soil samples. Such results can be explained both by the direct action of a consortium of phosphate-immobilizing and nitrogen-fixing bacteria and acidification of the medium due to an increase in phylum <i>Acidobacteriota</i> bacteria in the soil.https://www.mdpi.com/2223-7747/13/1/116consortia of microorganismsco-inoculationsoil microbiom |
| spellingShingle | Ekaterina Alexeevna Sokolova Olga Viktorovna Mishukova Inna Viktorovna Hlistun Irina Nikolaevna Tromenschleger Artem Yurievich Tikunov Andrey Dmitrievich Manakhov Evgeny Ivanovich Rogaev Oleg Alexandrovich Savenkov Maria Dmitrievna Buyanova Ilya Vladimirovich Ivanov Natalya Valentinovna Smirnova Elena Nikolaevna Voronina The Effectiveness of Co-Inoculation by Consortia of Microorganisms Depends on the Type of Plant and the Soil Microbiome Plants consortia of microorganisms co-inoculation soil microbiom |
| title | The Effectiveness of Co-Inoculation by Consortia of Microorganisms Depends on the Type of Plant and the Soil Microbiome |
| title_full | The Effectiveness of Co-Inoculation by Consortia of Microorganisms Depends on the Type of Plant and the Soil Microbiome |
| title_fullStr | The Effectiveness of Co-Inoculation by Consortia of Microorganisms Depends on the Type of Plant and the Soil Microbiome |
| title_full_unstemmed | The Effectiveness of Co-Inoculation by Consortia of Microorganisms Depends on the Type of Plant and the Soil Microbiome |
| title_short | The Effectiveness of Co-Inoculation by Consortia of Microorganisms Depends on the Type of Plant and the Soil Microbiome |
| title_sort | effectiveness of co inoculation by consortia of microorganisms depends on the type of plant and the soil microbiome |
| topic | consortia of microorganisms co-inoculation soil microbiom |
| url | https://www.mdpi.com/2223-7747/13/1/116 |
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