Emulsion templated composites: Porous nerve guidance conduits for peripheral nerve regeneration
Peripheral nerve regeneration fails following more severe injuries where the gap between nerve stumps is too large. Nerve guidance conduits (NGCs) can be implanted to connect the nerve stumps and provide a regenerative microenvironment. This study shows that poly(glycerol sebacate)-methacrylate (PGS...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Materials & Design |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127524001515 |
| _version_ | 1797256441381257216 |
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| author | Louis D. V. Johnson Mina Aleemardani Simon Atkins Fiona M. Boissonade Frederik Claeyssens |
| author_facet | Louis D. V. Johnson Mina Aleemardani Simon Atkins Fiona M. Boissonade Frederik Claeyssens |
| author_sort | Louis D. V. Johnson |
| collection | DOAJ |
| description | Peripheral nerve regeneration fails following more severe injuries where the gap between nerve stumps is too large. Nerve guidance conduits (NGCs) can be implanted to connect the nerve stumps and provide a regenerative microenvironment. This study shows that poly(glycerol sebacate)-methacrylate (PGS-M), a photocurable and biodegradable elastomer, can be used to fabricate porous NGCs. Further, novel developments in emulsion templating techniques are used to generate composite structures and their mechanical properties are investigated. The most appropriate composite for nerve tissue repair had a compressive and tensile Young’s modulus of 0.168 MPa and 0.694 MPa respectively, much closer to that of native nerve tissue than previously used polymers. SEM images revealed high porosity/interconnectivity of NGCs, important for the diffusion of nutrients to further aid nerve regeneration. FITC-Dextran (150 kDa) effectively diffused through conduit walls. Histological analysis demonstrated the small pore size successfully prevented infiltration of fibroblasts. In vitro studies indicated that the NGCs were cytocompatible. Ex vivo analysis showed that neurons of embryonic chick dorsal root ganglia grew on the surface of the conduits, and Schwann cells migrated effectively along the lumen. This study demonstrates a novel way of fabricating porous scaffolds and demonstrates their potential for the treatment of PNI. |
| first_indexed | 2024-03-07T22:01:28Z |
| format | Article |
| id | doaj.art-c78abadcb6b84b8f94ea6d70b3fc67a6 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-04-24T22:21:48Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-c78abadcb6b84b8f94ea6d70b3fc67a62024-03-20T06:08:20ZengElsevierMaterials & Design0264-12752024-03-01239112779Emulsion templated composites: Porous nerve guidance conduits for peripheral nerve regenerationLouis D. V. Johnson0Mina Aleemardani1Simon Atkins2Fiona M. Boissonade3Frederik Claeyssens4Kroto Research Institute, Department of Materials Science and Engineering, University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK; The Neuroscience Institute, University of Sheffield, Sheffield, UKKroto Research Institute, Department of Materials Science and Engineering, University of Sheffield, Broad Lane, Sheffield S3 7HQ, UKSchool of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TN, UK; The Neuroscience Institute, University of Sheffield, Sheffield, UKSchool of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TN, UK; The Neuroscience Institute, University of Sheffield, Sheffield, UKKroto Research Institute, Department of Materials Science and Engineering, University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK; The Neuroscience Institute, University of Sheffield, Sheffield, UK; Corresponding author at: Kroto Research Institute, Department of Materials Science and Engineering, University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK.Peripheral nerve regeneration fails following more severe injuries where the gap between nerve stumps is too large. Nerve guidance conduits (NGCs) can be implanted to connect the nerve stumps and provide a regenerative microenvironment. This study shows that poly(glycerol sebacate)-methacrylate (PGS-M), a photocurable and biodegradable elastomer, can be used to fabricate porous NGCs. Further, novel developments in emulsion templating techniques are used to generate composite structures and their mechanical properties are investigated. The most appropriate composite for nerve tissue repair had a compressive and tensile Young’s modulus of 0.168 MPa and 0.694 MPa respectively, much closer to that of native nerve tissue than previously used polymers. SEM images revealed high porosity/interconnectivity of NGCs, important for the diffusion of nutrients to further aid nerve regeneration. FITC-Dextran (150 kDa) effectively diffused through conduit walls. Histological analysis demonstrated the small pore size successfully prevented infiltration of fibroblasts. In vitro studies indicated that the NGCs were cytocompatible. Ex vivo analysis showed that neurons of embryonic chick dorsal root ganglia grew on the surface of the conduits, and Schwann cells migrated effectively along the lumen. This study demonstrates a novel way of fabricating porous scaffolds and demonstrates their potential for the treatment of PNI.http://www.sciencedirect.com/science/article/pii/S0264127524001515Nerve guidance conduitPorousComposite |
| spellingShingle | Louis D. V. Johnson Mina Aleemardani Simon Atkins Fiona M. Boissonade Frederik Claeyssens Emulsion templated composites: Porous nerve guidance conduits for peripheral nerve regeneration Materials & Design Nerve guidance conduit Porous Composite |
| title | Emulsion templated composites: Porous nerve guidance conduits for peripheral nerve regeneration |
| title_full | Emulsion templated composites: Porous nerve guidance conduits for peripheral nerve regeneration |
| title_fullStr | Emulsion templated composites: Porous nerve guidance conduits for peripheral nerve regeneration |
| title_full_unstemmed | Emulsion templated composites: Porous nerve guidance conduits for peripheral nerve regeneration |
| title_short | Emulsion templated composites: Porous nerve guidance conduits for peripheral nerve regeneration |
| title_sort | emulsion templated composites porous nerve guidance conduits for peripheral nerve regeneration |
| topic | Nerve guidance conduit Porous Composite |
| url | http://www.sciencedirect.com/science/article/pii/S0264127524001515 |
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