Zinc Oxide Nanoparticles Improve Pleioblastus pygmaeus Plant Tolerance to Arsenic and Mercury by Stimulating Antioxidant Defense and Reducing the Metal Accumulation and Translocation
The utilization of nanoparticles to potentially reduce toxicity from metals/metalloids in plants has increased in recent years, which can help them to achieve tolerance under the stressful conditions. An in vitro experiment was conducted to investigate five different levels of zinc oxide nanoparticl...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.841501/full |
| _version_ | 1818499009690468352 |
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| author | Abolghassem Emamverdian Abolghassem Emamverdian Mirza Hasanuzzaman Yulong Ding Yulong Ding James Barker Farzad Mokhberdoran Guohua Liu Guohua Liu |
| author_facet | Abolghassem Emamverdian Abolghassem Emamverdian Mirza Hasanuzzaman Yulong Ding Yulong Ding James Barker Farzad Mokhberdoran Guohua Liu Guohua Liu |
| author_sort | Abolghassem Emamverdian |
| collection | DOAJ |
| description | The utilization of nanoparticles to potentially reduce toxicity from metals/metalloids in plants has increased in recent years, which can help them to achieve tolerance under the stressful conditions. An in vitro experiment was conducted to investigate five different levels of zinc oxide nanoparticles (ZnO-NPs; 0, 50, 100, 150, and 200 μM) both alone and in combination with 150 μM arsenic (As) and 150 μM mercury (Hg) in one-year-old Pleioblastus pygmaeus (Miq.) Nakai plants through four replications. The results demonstrated that As and Hg alone had damaging effects on the plant growth and development. However, the addition of various concentrations of ZnO-NPs led to increased antioxidant activity, proline (79%) content, glycine betaine (71%) content, tyrosine ammonia-lyase (43%) activity, phenylalanine ammonia-lyase (69%) activity, chlorophyll indices, and eventually plant biomass, while the lipoxygenase activity, electrolyte leakage, soluble protein, hydrogen peroxide content, and thiobarbituric acid reactive substances were reduced. We concluded that ZnO-NPs detoxified As and Hg toxicity in the plants through increasing antioxidant activity, reducing As and Hg accumulation, As and Hg translocation from roots to shoots, and adjusting stomatal closure. This detoxification was further confirmed by the reduction of the translocation factor of As and Hg and the enhancement of the tolerance index in combination with ZnO-NPs. However, there is a need for further investigation with different metals/metalloids. |
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| format | Article |
| id | doaj.art-c78e7670d64f4b5c82ae1a6be5642727 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T20:23:17Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c78e7670d64f4b5c82ae1a6be56427272022-12-22T01:34:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-02-011310.3389/fpls.2022.841501841501Zinc Oxide Nanoparticles Improve Pleioblastus pygmaeus Plant Tolerance to Arsenic and Mercury by Stimulating Antioxidant Defense and Reducing the Metal Accumulation and TranslocationAbolghassem Emamverdian0Abolghassem Emamverdian1Mirza Hasanuzzaman2Yulong Ding3Yulong Ding4James Barker5Farzad Mokhberdoran6Guohua Liu7Guohua Liu8Co-innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaBamboo Research Institute, Nanjing Forestry University, Nanjing, ChinaDepartment of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, BangladeshCo-innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaBamboo Research Institute, Nanjing Forestry University, Nanjing, ChinaSchool of Life Sciences, Pharmacy and Chemistry, Kingston University, Kingston-Upon-Thames, United KingdomCo-innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaCo-innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaBamboo Research Institute, Nanjing Forestry University, Nanjing, ChinaThe utilization of nanoparticles to potentially reduce toxicity from metals/metalloids in plants has increased in recent years, which can help them to achieve tolerance under the stressful conditions. An in vitro experiment was conducted to investigate five different levels of zinc oxide nanoparticles (ZnO-NPs; 0, 50, 100, 150, and 200 μM) both alone and in combination with 150 μM arsenic (As) and 150 μM mercury (Hg) in one-year-old Pleioblastus pygmaeus (Miq.) Nakai plants through four replications. The results demonstrated that As and Hg alone had damaging effects on the plant growth and development. However, the addition of various concentrations of ZnO-NPs led to increased antioxidant activity, proline (79%) content, glycine betaine (71%) content, tyrosine ammonia-lyase (43%) activity, phenylalanine ammonia-lyase (69%) activity, chlorophyll indices, and eventually plant biomass, while the lipoxygenase activity, electrolyte leakage, soluble protein, hydrogen peroxide content, and thiobarbituric acid reactive substances were reduced. We concluded that ZnO-NPs detoxified As and Hg toxicity in the plants through increasing antioxidant activity, reducing As and Hg accumulation, As and Hg translocation from roots to shoots, and adjusting stomatal closure. This detoxification was further confirmed by the reduction of the translocation factor of As and Hg and the enhancement of the tolerance index in combination with ZnO-NPs. However, there is a need for further investigation with different metals/metalloids.https://www.frontiersin.org/articles/10.3389/fpls.2022.841501/fullmetal/metalloid toxicitynanomaterialstolerance indexROS metabolismtranslocation factor |
| spellingShingle | Abolghassem Emamverdian Abolghassem Emamverdian Mirza Hasanuzzaman Yulong Ding Yulong Ding James Barker Farzad Mokhberdoran Guohua Liu Guohua Liu Zinc Oxide Nanoparticles Improve Pleioblastus pygmaeus Plant Tolerance to Arsenic and Mercury by Stimulating Antioxidant Defense and Reducing the Metal Accumulation and Translocation Frontiers in Plant Science metal/metalloid toxicity nanomaterials tolerance index ROS metabolism translocation factor |
| title | Zinc Oxide Nanoparticles Improve Pleioblastus pygmaeus Plant Tolerance to Arsenic and Mercury by Stimulating Antioxidant Defense and Reducing the Metal Accumulation and Translocation |
| title_full | Zinc Oxide Nanoparticles Improve Pleioblastus pygmaeus Plant Tolerance to Arsenic and Mercury by Stimulating Antioxidant Defense and Reducing the Metal Accumulation and Translocation |
| title_fullStr | Zinc Oxide Nanoparticles Improve Pleioblastus pygmaeus Plant Tolerance to Arsenic and Mercury by Stimulating Antioxidant Defense and Reducing the Metal Accumulation and Translocation |
| title_full_unstemmed | Zinc Oxide Nanoparticles Improve Pleioblastus pygmaeus Plant Tolerance to Arsenic and Mercury by Stimulating Antioxidant Defense and Reducing the Metal Accumulation and Translocation |
| title_short | Zinc Oxide Nanoparticles Improve Pleioblastus pygmaeus Plant Tolerance to Arsenic and Mercury by Stimulating Antioxidant Defense and Reducing the Metal Accumulation and Translocation |
| title_sort | zinc oxide nanoparticles improve pleioblastus pygmaeus plant tolerance to arsenic and mercury by stimulating antioxidant defense and reducing the metal accumulation and translocation |
| topic | metal/metalloid toxicity nanomaterials tolerance index ROS metabolism translocation factor |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.841501/full |
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