Investigating the Effects of All-trans Retinoic Acid on Histopathology of Pancreas of streptozotocin -Induced Diabetes in C57BL/6 mice
Backgrounds & Objectives: Type 1 diabetes is an auto-immune disease and caused by insufficient insulin production by the body. All-trans Retinoic Acid (ATRA) is an antioxidant, anti-cancer and anti-inflammatory agent. The objective of the present study was to investigate the effects of ATRA on h...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fasa University of Medical Sciences
2018-04-01
|
| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-1430-en.pdf |
| _version_ | 1827812397021134848 |
|---|---|
| author | Farin Malekifard Nowruz Delirezh Rahim Hobbnaghi Hasan Malekinejad |
| author_facet | Farin Malekifard Nowruz Delirezh Rahim Hobbnaghi Hasan Malekinejad |
| author_sort | Farin Malekifard |
| collection | DOAJ |
| description | Backgrounds & Objectives: Type 1 diabetes is an auto-immune disease and caused by insufficient insulin production by the body. All-trans Retinoic Acid (ATRA) is an antioxidant, anti-cancer and anti-inflammatory agent. The objective of the present study was to investigate the effects of ATRA on histopathology of pancreas in diabetic mice.
Material & Methods: Diabetes was induced by multiple low-dose of streptozotocin injection (40 mg/kg/day for 5 consecutive days) in male C57BL/6 mice. After induction of diabetes, mice were treated with ATRA (20 mg/kg/day i.p.) for 21 days. On the last day, pancreases were isolated and stained with hematoxylin &eosin (H&E) and Gomeri aldehyde fuchsin (GAF) for histological analyses (the number of islets and β cells, diameter of islets) of pancreas.
Results: ATRA treatment in streptozotocin-induced diabetic mice was increased the mean diameter of islets and the number of islets and beta cells compared to the diabetic group. (p<0/05).
Conclusion: The administration of ATRA improved pancreas tissue during destruction of the pancreatic beta-cells in STZ-induced type 1 diabetes in mice. |
| first_indexed | 2024-03-11T23:17:55Z |
| format | Article |
| id | doaj.art-c78fa5d5e6cf4381ac408c4c3d174ce3 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:17:55Z |
| publishDate | 2018-04-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-c78fa5d5e6cf4381ac408c4c3d174ce32023-09-20T21:23:49ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232018-04-0181657665Investigating the Effects of All-trans Retinoic Acid on Histopathology of Pancreas of streptozotocin -Induced Diabetes in C57BL/6 miceFarin Malekifard0Nowruz Delirezh1Rahim Hobbnaghi2Hasan Malekinejad3 Department of Microbiology, Facutly of Veterinary Medicine, Urmia University, Urmia, Iran Department of Microbiology, Facutly of Veterinary Medicine, Urmia University, Urmia, Iran Department of Pathobiology, Facutly of Veterinary Medicine, Urmia University, Urmia, Iran Department of Pharmacology, University of Medical Sciences, Urmia, Iran Backgrounds & Objectives: Type 1 diabetes is an auto-immune disease and caused by insufficient insulin production by the body. All-trans Retinoic Acid (ATRA) is an antioxidant, anti-cancer and anti-inflammatory agent. The objective of the present study was to investigate the effects of ATRA on histopathology of pancreas in diabetic mice. Material & Methods: Diabetes was induced by multiple low-dose of streptozotocin injection (40 mg/kg/day for 5 consecutive days) in male C57BL/6 mice. After induction of diabetes, mice were treated with ATRA (20 mg/kg/day i.p.) for 21 days. On the last day, pancreases were isolated and stained with hematoxylin &eosin (H&E) and Gomeri aldehyde fuchsin (GAF) for histological analyses (the number of islets and β cells, diameter of islets) of pancreas. Results: ATRA treatment in streptozotocin-induced diabetic mice was increased the mean diameter of islets and the number of islets and beta cells compared to the diabetic group. (p<0/05). Conclusion: The administration of ATRA improved pancreas tissue during destruction of the pancreatic beta-cells in STZ-induced type 1 diabetes in mice.http://jabs.fums.ac.ir/article-1-1430-en.pdftype 1 diabetesall-trans retinoic acidpancreasestreptozotocin |
| spellingShingle | Farin Malekifard Nowruz Delirezh Rahim Hobbnaghi Hasan Malekinejad Investigating the Effects of All-trans Retinoic Acid on Histopathology of Pancreas of streptozotocin -Induced Diabetes in C57BL/6 mice Journal of Advanced Biomedical Sciences type 1 diabetes all-trans retinoic acid pancrease streptozotocin |
| title | Investigating the Effects of All-trans Retinoic Acid on Histopathology of Pancreas of streptozotocin -Induced Diabetes in C57BL/6 mice |
| title_full | Investigating the Effects of All-trans Retinoic Acid on Histopathology of Pancreas of streptozotocin -Induced Diabetes in C57BL/6 mice |
| title_fullStr | Investigating the Effects of All-trans Retinoic Acid on Histopathology of Pancreas of streptozotocin -Induced Diabetes in C57BL/6 mice |
| title_full_unstemmed | Investigating the Effects of All-trans Retinoic Acid on Histopathology of Pancreas of streptozotocin -Induced Diabetes in C57BL/6 mice |
| title_short | Investigating the Effects of All-trans Retinoic Acid on Histopathology of Pancreas of streptozotocin -Induced Diabetes in C57BL/6 mice |
| title_sort | investigating the effects of all trans retinoic acid on histopathology of pancreas of streptozotocin induced diabetes in c57bl 6 mice |
| topic | type 1 diabetes all-trans retinoic acid pancrease streptozotocin |
| url | http://jabs.fums.ac.ir/article-1-1430-en.pdf |
| work_keys_str_mv | AT farinmalekifard investigatingtheeffectsofalltransretinoicacidonhistopathologyofpancreasofstreptozotocininduceddiabetesinc57bl6mice AT nowruzdelirezh investigatingtheeffectsofalltransretinoicacidonhistopathologyofpancreasofstreptozotocininduceddiabetesinc57bl6mice AT rahimhobbnaghi investigatingtheeffectsofalltransretinoicacidonhistopathologyofpancreasofstreptozotocininduceddiabetesinc57bl6mice AT hasanmalekinejad investigatingtheeffectsofalltransretinoicacidonhistopathologyofpancreasofstreptozotocininduceddiabetesinc57bl6mice |