Sequencing of E2 and NS5A regions of HCV genotype 3a in Brazilian patients with chronic hepatitis
Hepatitis C virus (HCV) is a major cause of liver disease throughout the world. The NS5A and E2 proteins of HCV genotype 1 were reported to inhibit the double-stranded (ds) RNA-dependent protein kinase (PKR), which is involved in the cellular antiviral response induced by interferon (IFN). The respo...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2010-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100014 |
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| author | Fernanda de Mello Malta José Eymard Moraes de Medeiros-Filho Raymundo Soares de Azevedo Luzia Gonçalves Luiz Caetano da Silva Flair José Carrilho João Renato Rebello Pinho |
| author_facet | Fernanda de Mello Malta José Eymard Moraes de Medeiros-Filho Raymundo Soares de Azevedo Luzia Gonçalves Luiz Caetano da Silva Flair José Carrilho João Renato Rebello Pinho |
| author_sort | Fernanda de Mello Malta |
| collection | DOAJ |
| description | Hepatitis C virus (HCV) is a major cause of liver disease throughout the world. The NS5A and E2 proteins of HCV genotype 1 were reported to inhibit the double-stranded (ds) RNA-dependent protein kinase (PKR), which is involved in the cellular antiviral response induced by interferon (IFN). The response to IFN therapy is quite different between genotypes, with response rates among patients infected with types 2 and 3 that are two-three-fold higher than in patients infected with type 1. Interestingly, a significant percentage of HCV genotype 3-infected patients do not respond to treatment at all. The aim of this paper was to analyse the sequences of fragments of the E2 and NS5A regions from 33 outpatients infected with genotype 3a, including patients that have responded (SVR) or not responded (NR) to treatment. HCV RNA was extracted and amplified with specific primers for the NS5A and E2 regions and the PCR products were then sequenced. The sequences obtained covered amino acids (aa) 636-708 in E2 and in NS5A [including the IFN sensitivity determining region (ISDR), PKR-binding domain and extended V3 region)]. In the E2 and NS5A regions, we did observe aa changes among patients, but these changes were not statistically significant between the SVR and NR groups. In conclusion, our results suggest that the ISDR domain is not predictive of treatment success in patients infected with HCV genotype 3a. |
| first_indexed | 2024-03-12T08:51:16Z |
| format | Article |
| id | doaj.art-c790a4eb58ca47418462adfc5b05b93e |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:51:16Z |
| publishDate | 2010-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-c790a4eb58ca47418462adfc5b05b93e2023-09-02T16:18:07ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602010-02-011051929810.1590/S0074-02762010000100014Sequencing of E2 and NS5A regions of HCV genotype 3a in Brazilian patients with chronic hepatitisFernanda de Mello MaltaJosé Eymard Moraes de Medeiros-FilhoRaymundo Soares de AzevedoLuzia GonçalvesLuiz Caetano da SilvaFlair José CarrilhoJoão Renato Rebello PinhoHepatitis C virus (HCV) is a major cause of liver disease throughout the world. The NS5A and E2 proteins of HCV genotype 1 were reported to inhibit the double-stranded (ds) RNA-dependent protein kinase (PKR), which is involved in the cellular antiviral response induced by interferon (IFN). The response to IFN therapy is quite different between genotypes, with response rates among patients infected with types 2 and 3 that are two-three-fold higher than in patients infected with type 1. Interestingly, a significant percentage of HCV genotype 3-infected patients do not respond to treatment at all. The aim of this paper was to analyse the sequences of fragments of the E2 and NS5A regions from 33 outpatients infected with genotype 3a, including patients that have responded (SVR) or not responded (NR) to treatment. HCV RNA was extracted and amplified with specific primers for the NS5A and E2 regions and the PCR products were then sequenced. The sequences obtained covered amino acids (aa) 636-708 in E2 and in NS5A [including the IFN sensitivity determining region (ISDR), PKR-binding domain and extended V3 region)]. In the E2 and NS5A regions, we did observe aa changes among patients, but these changes were not statistically significant between the SVR and NR groups. In conclusion, our results suggest that the ISDR domain is not predictive of treatment success in patients infected with HCV genotype 3a.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100014E2 proteinhepatitis C virusinterferon sensitivity determining regionNS5A proteinPKR-eIF2a phosphorylation homology domain |
| spellingShingle | Fernanda de Mello Malta José Eymard Moraes de Medeiros-Filho Raymundo Soares de Azevedo Luzia Gonçalves Luiz Caetano da Silva Flair José Carrilho João Renato Rebello Pinho Sequencing of E2 and NS5A regions of HCV genotype 3a in Brazilian patients with chronic hepatitis Memorias do Instituto Oswaldo Cruz E2 protein hepatitis C virus interferon sensitivity determining region NS5A protein PKR-eIF2a phosphorylation homology domain |
| title | Sequencing of E2 and NS5A regions of HCV genotype 3a in Brazilian patients with chronic hepatitis |
| title_full | Sequencing of E2 and NS5A regions of HCV genotype 3a in Brazilian patients with chronic hepatitis |
| title_fullStr | Sequencing of E2 and NS5A regions of HCV genotype 3a in Brazilian patients with chronic hepatitis |
| title_full_unstemmed | Sequencing of E2 and NS5A regions of HCV genotype 3a in Brazilian patients with chronic hepatitis |
| title_short | Sequencing of E2 and NS5A regions of HCV genotype 3a in Brazilian patients with chronic hepatitis |
| title_sort | sequencing of e2 and ns5a regions of hcv genotype 3a in brazilian patients with chronic hepatitis |
| topic | E2 protein hepatitis C virus interferon sensitivity determining region NS5A protein PKR-eIF2a phosphorylation homology domain |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100014 |
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