Genetically superior European sea bass (Dicentrarchus labrax) and nutritional innovations: Effects of functional feeds on fish immune response, disease resistance, and gut microbiota
The objective of this study was to determine if selected fish genotypes could benefit from the use of functional additives in novel aqua feed formulations to improve growth performance, gut microbiota, immune response, and disease resistance in fish. Two batches of juvenile European sea bass selecte...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423002867 |
| _version_ | 1797388837568118784 |
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| author | Simona Rimoldi Daniel Montero Silvia Torrecillas Antonio Serradell Felix Acosta Pierrick Haffray Barbara Hostins Ramon Fontanillas François Allal Aline Bajek Genciana Terova |
| author_facet | Simona Rimoldi Daniel Montero Silvia Torrecillas Antonio Serradell Felix Acosta Pierrick Haffray Barbara Hostins Ramon Fontanillas François Allal Aline Bajek Genciana Terova |
| author_sort | Simona Rimoldi |
| collection | DOAJ |
| description | The objective of this study was to determine if selected fish genotypes could benefit from the use of functional additives in novel aqua feed formulations to improve growth performance, gut microbiota, immune response, and disease resistance in fish. Two batches of juvenile European sea bass selected for high growth (HG; selected sires x selected dams), and wild types (WT; wild sires x selected females) were fed a “future diet” coated with three different functional additives for 12 weeks as follows: (i) 2 weeks with a high dose, followed by (ii) 10 weeks with a low dose. The functional additives tested were a mixture of probiotics (PROB), organic acids (ORG), and phytogens (PHYTO). A pathogen challenge test (Vibrio anguillarum) and a stress condition (overcrowding) were performed after each dose. At the end of the feeding experiment, fish from the HG group performed better than fish from the WT group in terms of body weight, relative growth, SGR, and DGI. The results of the two challenge tests performed after two weeks of high dose and ten weeks of low dose showed a significant effect of diet on fish survival. GALT-associated gene expression analysis revealed an interaction between the genotype and diet for il-1β in the distal gut. Finally, regarding the gut microbiota, discriminant analysis showed no clear separation between fish fed the future diet and those fed the same diet with experimental additives. Nevertheless, the relative abundance of certain taxa varied between experimental groups. For example, fish fed the ORG diet had higher relative abundance of Streptococcus in both genotypes, whereas fish fed the PHYTO diet had higher abundance of Lactobacillales. In contrast, fish fed PROB had lower abundance of Pseudomonas and Acinetobacter. |
| first_indexed | 2024-03-08T22:46:37Z |
| format | Article |
| id | doaj.art-c7924cd1cae64de7ba5b09756cf1e175 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-08T22:46:37Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-c7924cd1cae64de7ba5b09756cf1e1752023-12-17T06:39:32ZengElsevierAquaculture Reports2352-51342023-12-0133101747Genetically superior European sea bass (Dicentrarchus labrax) and nutritional innovations: Effects of functional feeds on fish immune response, disease resistance, and gut microbiotaSimona Rimoldi0Daniel Montero1Silvia Torrecillas2Antonio Serradell3Felix Acosta4Pierrick Haffray5Barbara Hostins6Ramon Fontanillas7François Allal8Aline Bajek9Genciana Terova10Department of Biotechnology and Life Sciences, University of Insubria, Via J. H. Dunant, 3, 21100 Varese, ItalyGrupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Telde, Las Palmas, Canary Islands, SpainInstitut de Recerca i Tecnologia Agroalimentàries–IRTA, Barcelona, SpainGrupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Telde, Las Palmas, Canary Islands, SpainGrupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Telde, Las Palmas, Canary Islands, SpainSYSAAF (French Poultry and Aquaculture Breeders Technical Centre), 35042 Rennes, FranceINVE Aquaculture, Dendermonde, BelgiumSkretting Aquaculture Research Centre, Stavanger, NorwayMARBEC, University of Montpellier, CNRS, Ifremer, IRD, 34250 Palavas-les-Flots, FranceEcoserie Marine de Graveline Ichtus, Route des Enrochements, 59820 Gravelines, FranceDepartment of Biotechnology and Life Sciences, University of Insubria, Via J. H. Dunant, 3, 21100 Varese, Italy; Corresponding author.The objective of this study was to determine if selected fish genotypes could benefit from the use of functional additives in novel aqua feed formulations to improve growth performance, gut microbiota, immune response, and disease resistance in fish. Two batches of juvenile European sea bass selected for high growth (HG; selected sires x selected dams), and wild types (WT; wild sires x selected females) were fed a “future diet” coated with three different functional additives for 12 weeks as follows: (i) 2 weeks with a high dose, followed by (ii) 10 weeks with a low dose. The functional additives tested were a mixture of probiotics (PROB), organic acids (ORG), and phytogens (PHYTO). A pathogen challenge test (Vibrio anguillarum) and a stress condition (overcrowding) were performed after each dose. At the end of the feeding experiment, fish from the HG group performed better than fish from the WT group in terms of body weight, relative growth, SGR, and DGI. The results of the two challenge tests performed after two weeks of high dose and ten weeks of low dose showed a significant effect of diet on fish survival. GALT-associated gene expression analysis revealed an interaction between the genotype and diet for il-1β in the distal gut. Finally, regarding the gut microbiota, discriminant analysis showed no clear separation between fish fed the future diet and those fed the same diet with experimental additives. Nevertheless, the relative abundance of certain taxa varied between experimental groups. For example, fish fed the ORG diet had higher relative abundance of Streptococcus in both genotypes, whereas fish fed the PHYTO diet had higher abundance of Lactobacillales. In contrast, fish fed PROB had lower abundance of Pseudomonas and Acinetobacter.http://www.sciencedirect.com/science/article/pii/S2352513423002867BacilliDNA barcodingFirmicutesMetagenomicsProteobacteria |
| spellingShingle | Simona Rimoldi Daniel Montero Silvia Torrecillas Antonio Serradell Felix Acosta Pierrick Haffray Barbara Hostins Ramon Fontanillas François Allal Aline Bajek Genciana Terova Genetically superior European sea bass (Dicentrarchus labrax) and nutritional innovations: Effects of functional feeds on fish immune response, disease resistance, and gut microbiota Aquaculture Reports Bacilli DNA barcoding Firmicutes Metagenomics Proteobacteria |
| title | Genetically superior European sea bass (Dicentrarchus labrax) and nutritional innovations: Effects of functional feeds on fish immune response, disease resistance, and gut microbiota |
| title_full | Genetically superior European sea bass (Dicentrarchus labrax) and nutritional innovations: Effects of functional feeds on fish immune response, disease resistance, and gut microbiota |
| title_fullStr | Genetically superior European sea bass (Dicentrarchus labrax) and nutritional innovations: Effects of functional feeds on fish immune response, disease resistance, and gut microbiota |
| title_full_unstemmed | Genetically superior European sea bass (Dicentrarchus labrax) and nutritional innovations: Effects of functional feeds on fish immune response, disease resistance, and gut microbiota |
| title_short | Genetically superior European sea bass (Dicentrarchus labrax) and nutritional innovations: Effects of functional feeds on fish immune response, disease resistance, and gut microbiota |
| title_sort | genetically superior european sea bass dicentrarchus labrax and nutritional innovations effects of functional feeds on fish immune response disease resistance and gut microbiota |
| topic | Bacilli DNA barcoding Firmicutes Metagenomics Proteobacteria |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423002867 |
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