Profiling Chromatin Accessibility Responses in Goat Bronchial Epithelial Cells Infected with <i>Pasteurella multocida</i>
<i>Pasteurella multocida</i> can cause goat hemorrhagic sepsis and endemic pneumonia. Respiratory epithelial cells are the first line of defense in the lungs during <i>P. multocida</i> infection. These cells act as a mechanical barrier and activate immune response to protect...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/2/1312 |
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| author | Qiaoling Chen Zhen Chen Zhenxing Zhang Haoju Pan Hong Li Xubo Li Qi An Yiwen Cheng Si Chen Churiga Man Li Du Fengyang Wang |
| author_facet | Qiaoling Chen Zhen Chen Zhenxing Zhang Haoju Pan Hong Li Xubo Li Qi An Yiwen Cheng Si Chen Churiga Man Li Du Fengyang Wang |
| author_sort | Qiaoling Chen |
| collection | DOAJ |
| description | <i>Pasteurella multocida</i> can cause goat hemorrhagic sepsis and endemic pneumonia. Respiratory epithelial cells are the first line of defense in the lungs during <i>P. multocida</i> infection. These cells act as a mechanical barrier and activate immune response to protect against invading pathogenic microorganisms. Upon infection, <i>P. multocida</i> adheres to the cells and causes changes in cell morphology and transcriptome. ATAC-seq was conducted to determine the changes in the chromatin open region of <i>P. multocida</i>-infected goat bronchial epithelial cells based on transcriptional regulation. A total of 13,079 and 28,722 peaks were identified in the control (CK) and treatment (T) groups (P. multocida infection group), respectively. The peaks significantly increased after <i>P. multocida</i> infection. The specific peaks for the CK and T groups were annotated to 545 and 6632 genes, respectively. KEGG pathway enrichment analysis revealed that the specific peak-related genes in the T group were enriched in immune reaction-related pathways, such as Fc gamma R-mediated phagocytosis, MAPK signaling pathway, bacterial invasion of epithelial cells, endocytosis, and autophagy pathways. Other cellular component pathways were also enriched, including the regulation of actin cytoskeleton, adherent junction, tight junction, and focal adhesion. The differential peaks between the two groups were subsequently analyzed. Compared to those in the CK group, 863 and 11 peaks were upregulated and downregulated, respectively, after the <i>P. multocida</i> infection. Fifty-six known transcription factor motifs were revealed in upregulated peaks in the <i>P. multocida</i>-infected group. By integrating ATAC-seq and RNA-seq, some candidate genes (SETBP1, RASGEF1B, CREB5, IRF5, TNF, CD70) that might be involved in the goat bronchial epithelial cell immune reaction to <i>P. multocida</i> infection were identified. Overall, <i>P. multocida</i> infection changed the structure of the cell and caused chromatin open regions to be upregulated. In addition, <i>P. multocida</i> infection actively mobilized the host immune response with the inflammatory phenotype. The findings provide valuable information for understanding the regulatory mechanisms of <i>P. multocida</i>-infected goat bronchial epithelial cells. |
| first_indexed | 2024-03-09T12:23:07Z |
| format | Article |
| id | doaj.art-c793a1df6ac44c57a550aa86558d78ef |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:23:07Z |
| publishDate | 2023-01-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c793a1df6ac44c57a550aa86558d78ef2023-11-30T22:38:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01242131210.3390/ijms24021312Profiling Chromatin Accessibility Responses in Goat Bronchial Epithelial Cells Infected with <i>Pasteurella multocida</i>Qiaoling Chen0Zhen Chen1Zhenxing Zhang2Haoju Pan3Hong Li4Xubo Li5Qi An6Yiwen Cheng7Si Chen8Churiga Man9Li Du10Fengyang Wang11Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, School of Animal Science and Technology, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, School of Animal Science and Technology, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, School of Animal Science and Technology, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, School of Animal Science and Technology, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, School of Animal Science and Technology, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, School of Animal Science and Technology, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, School of Animal Science and Technology, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, School of Animal Science and Technology, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, School of Animal Science and Technology, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, School of Animal Science and Technology, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, School of Animal Science and Technology, Hainan University, Haikou 570228, ChinaHainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, School of Animal Science and Technology, Hainan University, Haikou 570228, China<i>Pasteurella multocida</i> can cause goat hemorrhagic sepsis and endemic pneumonia. Respiratory epithelial cells are the first line of defense in the lungs during <i>P. multocida</i> infection. These cells act as a mechanical barrier and activate immune response to protect against invading pathogenic microorganisms. Upon infection, <i>P. multocida</i> adheres to the cells and causes changes in cell morphology and transcriptome. ATAC-seq was conducted to determine the changes in the chromatin open region of <i>P. multocida</i>-infected goat bronchial epithelial cells based on transcriptional regulation. A total of 13,079 and 28,722 peaks were identified in the control (CK) and treatment (T) groups (P. multocida infection group), respectively. The peaks significantly increased after <i>P. multocida</i> infection. The specific peaks for the CK and T groups were annotated to 545 and 6632 genes, respectively. KEGG pathway enrichment analysis revealed that the specific peak-related genes in the T group were enriched in immune reaction-related pathways, such as Fc gamma R-mediated phagocytosis, MAPK signaling pathway, bacterial invasion of epithelial cells, endocytosis, and autophagy pathways. Other cellular component pathways were also enriched, including the regulation of actin cytoskeleton, adherent junction, tight junction, and focal adhesion. The differential peaks between the two groups were subsequently analyzed. Compared to those in the CK group, 863 and 11 peaks were upregulated and downregulated, respectively, after the <i>P. multocida</i> infection. Fifty-six known transcription factor motifs were revealed in upregulated peaks in the <i>P. multocida</i>-infected group. By integrating ATAC-seq and RNA-seq, some candidate genes (SETBP1, RASGEF1B, CREB5, IRF5, TNF, CD70) that might be involved in the goat bronchial epithelial cell immune reaction to <i>P. multocida</i> infection were identified. Overall, <i>P. multocida</i> infection changed the structure of the cell and caused chromatin open regions to be upregulated. In addition, <i>P. multocida</i> infection actively mobilized the host immune response with the inflammatory phenotype. The findings provide valuable information for understanding the regulatory mechanisms of <i>P. multocida</i>-infected goat bronchial epithelial cells.https://www.mdpi.com/1422-0067/24/2/1312ATAC-seqchromatin accessibility responsesgoat bronchial epithelial cell<i>Pasteurella multocida</i>immune reaction |
| spellingShingle | Qiaoling Chen Zhen Chen Zhenxing Zhang Haoju Pan Hong Li Xubo Li Qi An Yiwen Cheng Si Chen Churiga Man Li Du Fengyang Wang Profiling Chromatin Accessibility Responses in Goat Bronchial Epithelial Cells Infected with <i>Pasteurella multocida</i> International Journal of Molecular Sciences ATAC-seq chromatin accessibility responses goat bronchial epithelial cell <i>Pasteurella multocida</i> immune reaction |
| title | Profiling Chromatin Accessibility Responses in Goat Bronchial Epithelial Cells Infected with <i>Pasteurella multocida</i> |
| title_full | Profiling Chromatin Accessibility Responses in Goat Bronchial Epithelial Cells Infected with <i>Pasteurella multocida</i> |
| title_fullStr | Profiling Chromatin Accessibility Responses in Goat Bronchial Epithelial Cells Infected with <i>Pasteurella multocida</i> |
| title_full_unstemmed | Profiling Chromatin Accessibility Responses in Goat Bronchial Epithelial Cells Infected with <i>Pasteurella multocida</i> |
| title_short | Profiling Chromatin Accessibility Responses in Goat Bronchial Epithelial Cells Infected with <i>Pasteurella multocida</i> |
| title_sort | profiling chromatin accessibility responses in goat bronchial epithelial cells infected with i pasteurella multocida i |
| topic | ATAC-seq chromatin accessibility responses goat bronchial epithelial cell <i>Pasteurella multocida</i> immune reaction |
| url | https://www.mdpi.com/1422-0067/24/2/1312 |
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