Profile of PKS and NRPS Gene Clusters in the Genome of <i>Streptomyces cellostaticus</i> NBRC 12849<sup>T</sup>
Polyketides and nonribosomal peptides are major secondary metabolites in members of the genus <i>Streptomyces</i>. <i>Streptomyces cellostaticus</i> is a validly recognized species and the type strain produces cellostatin. However, little is known about whether it has the pot...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/11/924 |
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| author | Hisayuki Komaki Tomohiko Tamura |
| author_facet | Hisayuki Komaki Tomohiko Tamura |
| author_sort | Hisayuki Komaki |
| collection | DOAJ |
| description | Polyketides and nonribosomal peptides are major secondary metabolites in members of the genus <i>Streptomyces</i>. <i>Streptomyces cellostaticus</i> is a validly recognized species and the type strain produces cellostatin. However, little is known about whether it has the potential to produce diverse polyketides and nonribosomal peptides. Here, we sequenced the whole genome of <i>S. cellostaticus</i> NBRC 12849<sup>T</sup> and surveyed polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) gene clusters in the genome. The genome encoded 12 PKS, one NRPS and eight hybrid PKS/NRPS gene clusters. Among the 21 gene clusters, products of 10 gene clusters were annotated to be an annimycin congener, fuelimycins, lankamycin, streptovaricin, spore pigment, flaviolin, foxicin, blasticidin, lankacidin and an incarnatapeptine congener via our bioinformatic analysis. Although the other clusters were orphan and their products were unknown, five of them were predicted to be compounds derived from two independent diketides, a tridecaketide, a triketide and a tetraketide with a cysteine residue, respectively. These results suggest that <i>S. cellostaticus</i> is a source of diverse polyketides and hybrid polyketide/nonribosomal peptides, including unknown and new secondary metabolites. |
| first_indexed | 2024-03-09T16:51:12Z |
| format | Article |
| id | doaj.art-c79578e1e8154ae2a02c20e76c6b4398 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T16:51:12Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-c79578e1e8154ae2a02c20e76c6b43982023-11-24T14:41:12ZengMDPI AGFermentation2311-56372023-10-0191192410.3390/fermentation9110924Profile of PKS and NRPS Gene Clusters in the Genome of <i>Streptomyces cellostaticus</i> NBRC 12849<sup>T</sup>Hisayuki Komaki0Tomohiko Tamura1Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba 292-0818, JapanBiological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba 292-0818, JapanPolyketides and nonribosomal peptides are major secondary metabolites in members of the genus <i>Streptomyces</i>. <i>Streptomyces cellostaticus</i> is a validly recognized species and the type strain produces cellostatin. However, little is known about whether it has the potential to produce diverse polyketides and nonribosomal peptides. Here, we sequenced the whole genome of <i>S. cellostaticus</i> NBRC 12849<sup>T</sup> and surveyed polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) gene clusters in the genome. The genome encoded 12 PKS, one NRPS and eight hybrid PKS/NRPS gene clusters. Among the 21 gene clusters, products of 10 gene clusters were annotated to be an annimycin congener, fuelimycins, lankamycin, streptovaricin, spore pigment, flaviolin, foxicin, blasticidin, lankacidin and an incarnatapeptine congener via our bioinformatic analysis. Although the other clusters were orphan and their products were unknown, five of them were predicted to be compounds derived from two independent diketides, a tridecaketide, a triketide and a tetraketide with a cysteine residue, respectively. These results suggest that <i>S. cellostaticus</i> is a source of diverse polyketides and hybrid polyketide/nonribosomal peptides, including unknown and new secondary metabolites.https://www.mdpi.com/2311-5637/9/11/924blasticidingenomelankacidinlankamycinnonribosomal peptidepolyketide |
| spellingShingle | Hisayuki Komaki Tomohiko Tamura Profile of PKS and NRPS Gene Clusters in the Genome of <i>Streptomyces cellostaticus</i> NBRC 12849<sup>T</sup> Fermentation blasticidin genome lankacidin lankamycin nonribosomal peptide polyketide |
| title | Profile of PKS and NRPS Gene Clusters in the Genome of <i>Streptomyces cellostaticus</i> NBRC 12849<sup>T</sup> |
| title_full | Profile of PKS and NRPS Gene Clusters in the Genome of <i>Streptomyces cellostaticus</i> NBRC 12849<sup>T</sup> |
| title_fullStr | Profile of PKS and NRPS Gene Clusters in the Genome of <i>Streptomyces cellostaticus</i> NBRC 12849<sup>T</sup> |
| title_full_unstemmed | Profile of PKS and NRPS Gene Clusters in the Genome of <i>Streptomyces cellostaticus</i> NBRC 12849<sup>T</sup> |
| title_short | Profile of PKS and NRPS Gene Clusters in the Genome of <i>Streptomyces cellostaticus</i> NBRC 12849<sup>T</sup> |
| title_sort | profile of pks and nrps gene clusters in the genome of i streptomyces cellostaticus i nbrc 12849 sup t sup |
| topic | blasticidin genome lankacidin lankamycin nonribosomal peptide polyketide |
| url | https://www.mdpi.com/2311-5637/9/11/924 |
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