TECHNOLOGY OF MISCANTHUS BIOMASS SACCHARIFICATION WITH COMMERCIALLY AVAILABLE ENZYMES

We analyzed the possibility of using commercially available enzymes with cellulosolytic activity for saccharification of miscanthus biomass, Soranovsky variety, a new crop registered in Russia in 2013, in comparison to the saccharification of biomasses of Phalaris arundinacea, Thrachomitum lancifoli...

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Bibliographic Details
Main Authors: T. N. Goryachkovskaya, K. V. Starostin, I. A. Meshcheryakova, N. M. Slynko, S. E. Peltek
Format: Article
Language:English
Published: Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders 2015-01-01
Series:Вавиловский журнал генетики и селекции
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Online Access:https://vavilov.elpub.ru/jour/article/view/329
Description
Summary:We analyzed the possibility of using commercially available enzymes with cellulosolytic activity for saccharification of miscanthus biomass, Soranovsky variety, a new crop registered in Russia in 2013, in comparison to the saccharification of biomasses of Phalaris arundinacea, Thrachomitum lancifolium, and Sida hermaphrodita. For enzymatic hydrolysis, we used commercially available fungal cellulases: Thermomyces lanuginosus xylanase, Aspergillus niger cellulase, and Pen. verruculosum cellobiase and cellulase. A biomass was ground and incubated in alkaline peroxide. The highest rate of hydrolysis was observed with the Phalaris arundinacea biomass. We tested various combinations of enzymes and achieved 100 % conversion for all samples relative to the weight of hydrolyzable components, which corresponds to 70 % conversion of biomass.
ISSN:2500-3259