TECHNOLOGY OF MISCANTHUS BIOMASS SACCHARIFICATION WITH COMMERCIALLY AVAILABLE ENZYMES
We analyzed the possibility of using commercially available enzymes with cellulosolytic activity for saccharification of miscanthus biomass, Soranovsky variety, a new crop registered in Russia in 2013, in comparison to the saccharification of biomasses of Phalaris arundinacea, Thrachomitum lancifoli...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-01-01
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| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/329 |
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| author | T. N. Goryachkovskaya K. V. Starostin I. A. Meshcheryakova N. M. Slynko S. E. Peltek |
| author_facet | T. N. Goryachkovskaya K. V. Starostin I. A. Meshcheryakova N. M. Slynko S. E. Peltek |
| author_sort | T. N. Goryachkovskaya |
| collection | DOAJ |
| description | We analyzed the possibility of using commercially available enzymes with cellulosolytic activity for saccharification of miscanthus biomass, Soranovsky variety, a new crop registered in Russia in 2013, in comparison to the saccharification of biomasses of Phalaris arundinacea, Thrachomitum lancifolium, and Sida hermaphrodita. For enzymatic hydrolysis, we used commercially available fungal cellulases: Thermomyces lanuginosus xylanase, Aspergillus niger cellulase, and Pen. verruculosum cellobiase and cellulase. A biomass was ground and incubated in alkaline peroxide. The highest rate of hydrolysis was observed with the Phalaris arundinacea biomass. We tested various combinations of enzymes and achieved 100 % conversion for all samples relative to the weight of hydrolyzable components, which corresponds to 70 % conversion of biomass. |
| first_indexed | 2024-03-07T16:07:29Z |
| format | Article |
| id | doaj.art-c7986d1ab9544216ab135229193541f8 |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:09:59Z |
| publishDate | 2015-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-c7986d1ab9544216ab135229193541f82024-04-11T15:30:55ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-01-01184/2983988312TECHNOLOGY OF MISCANTHUS BIOMASS SACCHARIFICATION WITH COMMERCIALLY AVAILABLE ENZYMEST. N. Goryachkovskaya0K. V. Starostin1I. A. Meshcheryakova2N. M. Slynko3S. E. Peltek4Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia Sunline LLC, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Sunline LLC, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Sunline LLC, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Sunline LLC, Novosibirsk, RussiaWe analyzed the possibility of using commercially available enzymes with cellulosolytic activity for saccharification of miscanthus biomass, Soranovsky variety, a new crop registered in Russia in 2013, in comparison to the saccharification of biomasses of Phalaris arundinacea, Thrachomitum lancifolium, and Sida hermaphrodita. For enzymatic hydrolysis, we used commercially available fungal cellulases: Thermomyces lanuginosus xylanase, Aspergillus niger cellulase, and Pen. verruculosum cellobiase and cellulase. A biomass was ground and incubated in alkaline peroxide. The highest rate of hydrolysis was observed with the Phalaris arundinacea biomass. We tested various combinations of enzymes and achieved 100 % conversion for all samples relative to the weight of hydrolyzable components, which corresponds to 70 % conversion of biomass.https://vavilov.elpub.ru/jour/article/view/329miscanthushydrolysis of plant biomass“green chemistry”glycosidehydrolasecellulasexylanase |
| spellingShingle | T. N. Goryachkovskaya K. V. Starostin I. A. Meshcheryakova N. M. Slynko S. E. Peltek TECHNOLOGY OF MISCANTHUS BIOMASS SACCHARIFICATION WITH COMMERCIALLY AVAILABLE ENZYMES Вавиловский журнал генетики и селекции miscanthus hydrolysis of plant biomass “green chemistry” glycoside hydrolase cellulase xylanase |
| title | TECHNOLOGY OF MISCANTHUS BIOMASS SACCHARIFICATION WITH COMMERCIALLY AVAILABLE ENZYMES |
| title_full | TECHNOLOGY OF MISCANTHUS BIOMASS SACCHARIFICATION WITH COMMERCIALLY AVAILABLE ENZYMES |
| title_fullStr | TECHNOLOGY OF MISCANTHUS BIOMASS SACCHARIFICATION WITH COMMERCIALLY AVAILABLE ENZYMES |
| title_full_unstemmed | TECHNOLOGY OF MISCANTHUS BIOMASS SACCHARIFICATION WITH COMMERCIALLY AVAILABLE ENZYMES |
| title_short | TECHNOLOGY OF MISCANTHUS BIOMASS SACCHARIFICATION WITH COMMERCIALLY AVAILABLE ENZYMES |
| title_sort | technology of miscanthus biomass saccharification with commercially available enzymes |
| topic | miscanthus hydrolysis of plant biomass “green chemistry” glycoside hydrolase cellulase xylanase |
| url | https://vavilov.elpub.ru/jour/article/view/329 |
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