Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio)
Highlihgt • Develop primary cultures derived From tissue tails fins, gills, kidney and spleen from local Indonesian carp (Cyprinus carpio). • Primary culture cell with L15 Media • cell cultures consist of two type Fibroblast-like and epithelial –like cell Abstract The fish cell lines technology...
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| Format: | Article |
| Language: | Indonesian |
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Faculty of Fisheries and Marine Universitas Airlangga
2020-03-01
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| Series: | Jurnal Ilmiah Perikanan dan Kelautan |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/JIPK/article/view/17656 |
| _version_ | 1818205037482999808 |
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| author | Insariani Insariani Trisniaty Trisniaty Freddy Riatmono Abdul Ghani |
| author_facet | Insariani Insariani Trisniaty Trisniaty Freddy Riatmono Abdul Ghani |
| author_sort | Insariani Insariani |
| collection | DOAJ |
| description | Highlihgt
• Develop primary cultures derived From tissue tails fins, gills, kidney and spleen from local Indonesian carp (Cyprinus carpio).
• Primary culture cell with L15 Media
• cell cultures consist of two type Fibroblast-like and epithelial –like cell
Abstract
The fish cell lines technology have been developed for the interests of the fisheries world. This study aimed at developing a primary cell line from gill, kidney, spleen, and caudal fin of a common carp (Cyprinus carpio). A healthy common carp weighing 20 g (~1 month) was collected from the Cijeruk Fish Seed Center, Bogor. The development of primary cell lines from the gill, fin, tail, kidney and spleen tissue was performed in cell culture medium Leibovitz’s L-15 supplemented with 20% serum fetal bovine, 250 IU Penicillin, 250 µg / ml kanamycin sulfate and 2Mm L-Glutamine, and incubated at 28°C. Primary cell lines of caudal fin and gill began to form a monolayer on day 17 after culture. While the development of cell lines from kidney and spleen, although the initiation of cells and cells spread on the surface into a monolayer, was not perfect; therefore, the passage was unable to be done. Microscopic observations and Giemsa staining showed primary cell lines of caudal fin and gill based on cell morphology consisted of two cell types, fibroblast-like cells and epithelial-like cells. The first passage was done on day 17 when the confluence was more than 50%. The next passage was carried out every 3 weeks when confluence reached 70% -80%. The primary cell culture of gill was successfully passaged as much as 72 and the caudal fin was successfully passed as much as 89 times over 7 years. These new cell lines can be further used to propagate fish viruses and other biotechnology assays. |
| first_indexed | 2024-12-12T03:50:46Z |
| format | Article |
| id | doaj.art-c79974bdbbd74aa99b92fdbd9fbe48c8 |
| institution | Directory Open Access Journal |
| issn | 2085-5842 2528-0759 |
| language | Indonesian |
| last_indexed | 2024-12-12T03:50:46Z |
| publishDate | 2020-03-01 |
| publisher | Faculty of Fisheries and Marine Universitas Airlangga |
| record_format | Article |
| series | Jurnal Ilmiah Perikanan dan Kelautan |
| spelling | doaj.art-c79974bdbbd74aa99b92fdbd9fbe48c82022-12-22T00:39:24ZindFaculty of Fisheries and Marine Universitas AirlanggaJurnal Ilmiah Perikanan dan Kelautan2085-58422528-07592020-03-01121738010.20473/jipk.v12i1.176568759Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio)Insariani Insariani0Trisniaty Trisniaty1Freddy Riatmono2Abdul Ghani3Balai Uji Standar Karantina Ikan Pengendalian Mutu Dan Keamanan Hasil Perikanan, Marine and Fisheries Ministry. IndonesiaBalai Uji Standar Karantina Ikan Pengendalian Mutu Dan Keamanan Hasil Perikanan, Marine and Fisheries Ministry. IndonesiaBalai Uji Standar Karantina Ikan Pengendalian Mutu Dan Keamanan Hasil Perikanan, Marine and Fisheries Ministry. IndonesiaFakultas kedokteran hewan, Institut Pertanian Bogor. IndonesiaHighlihgt • Develop primary cultures derived From tissue tails fins, gills, kidney and spleen from local Indonesian carp (Cyprinus carpio). • Primary culture cell with L15 Media • cell cultures consist of two type Fibroblast-like and epithelial –like cell Abstract The fish cell lines technology have been developed for the interests of the fisheries world. This study aimed at developing a primary cell line from gill, kidney, spleen, and caudal fin of a common carp (Cyprinus carpio). A healthy common carp weighing 20 g (~1 month) was collected from the Cijeruk Fish Seed Center, Bogor. The development of primary cell lines from the gill, fin, tail, kidney and spleen tissue was performed in cell culture medium Leibovitz’s L-15 supplemented with 20% serum fetal bovine, 250 IU Penicillin, 250 µg / ml kanamycin sulfate and 2Mm L-Glutamine, and incubated at 28°C. Primary cell lines of caudal fin and gill began to form a monolayer on day 17 after culture. While the development of cell lines from kidney and spleen, although the initiation of cells and cells spread on the surface into a monolayer, was not perfect; therefore, the passage was unable to be done. Microscopic observations and Giemsa staining showed primary cell lines of caudal fin and gill based on cell morphology consisted of two cell types, fibroblast-like cells and epithelial-like cells. The first passage was done on day 17 when the confluence was more than 50%. The next passage was carried out every 3 weeks when confluence reached 70% -80%. The primary cell culture of gill was successfully passaged as much as 72 and the caudal fin was successfully passed as much as 89 times over 7 years. These new cell lines can be further used to propagate fish viruses and other biotechnology assays.https://e-journal.unair.ac.id/JIPK/article/view/17656cell lines, caudal fin, cyprinus carpio, gills |
| spellingShingle | Insariani Insariani Trisniaty Trisniaty Freddy Riatmono Abdul Ghani Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio) Jurnal Ilmiah Perikanan dan Kelautan cell lines, caudal fin, cyprinus carpio, gills |
| title | Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio) |
| title_full | Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio) |
| title_fullStr | Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio) |
| title_full_unstemmed | Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio) |
| title_short | Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio) |
| title_sort | development of primary cell lines from gill kidney spleen and caudal fin of common carp cyprinus carpio |
| topic | cell lines, caudal fin, cyprinus carpio, gills |
| url | https://e-journal.unair.ac.id/JIPK/article/view/17656 |
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