Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device
Detecting Ganoderma boninense in Indonesia is crucial for effectively controlling and mitigating the spread of basal stem disease in oil palm fields. While there is ongoing development of tolerant plants, no such plant has been successfully created yet. Consequently, researchers are actively studyin...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada, Yogyakarta
2024-03-01
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| Series: | Indonesian Journal of Biotechnology |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/83645 |
| _version_ | 1827300485804064768 |
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| author | Adella Josephin Yudan Whulanza Siti Fauziyah Rahman Kenny Lischer Muhammad Imam Surya Irfan Martiansyah Wiguna Rahman Uda Hashim |
| author_facet | Adella Josephin Yudan Whulanza Siti Fauziyah Rahman Kenny Lischer Muhammad Imam Surya Irfan Martiansyah Wiguna Rahman Uda Hashim |
| author_sort | Adella Josephin |
| collection | DOAJ |
| description | Detecting Ganoderma boninense in Indonesia is crucial for effectively controlling and mitigating the spread of basal stem disease in oil palm fields. While there is ongoing development of tolerant plants, no such plant has been successfully created yet. Consequently, researchers are actively studying detection methods for Ganoderma boninense. One established and highly accurate approach is the use of polymerase chain reaction (PCR) techniques for molecular detection. However, this method requires time‐consuming sample preparation, which can pose challenges in plantation settings. To address this problem, a portable lab‐on‐chip device has been introduced. This technology enables easy and automatic DNA retrieval from liquid samples by absorbing lysed DNA using magnetic beads. An efficient mechanism for manipulating the magnetic bead within the semiconductor has been successfully implemented. The extraction process typically takes around 15 minutes using a modified methodology on the chip device approach. The chip facilitates the retrieval of two samples with a capacity of 120 µL for each sample. The PCR method was utilized to validate the equivalence of the lab‐on‐chip device extraction to the standard extraction method. This represents a promising alternative for expedited and simplified detection of Ganoderma boninense in field conditions. |
| first_indexed | 2024-04-24T16:05:38Z |
| format | Article |
| id | doaj.art-c79a8f7694ec4de8aef63f53c8b40499 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-04-24T16:05:38Z |
| publishDate | 2024-03-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-c79a8f7694ec4de8aef63f53c8b404992024-04-01T05:27:54ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412024-03-01291333910.22146/ijbiotech.8364535476Easy extraction of Ganoderma boninense liquid sample using portable on‐chip deviceAdella Josephin0Yudan Whulanza1Siti Fauziyah Rahman2Kenny Lischer3Muhammad Imam Surya4Irfan Martiansyah5Wiguna Rahman6Uda Hashim7Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia; Research Center of Biomedical Engineering, Universitas Indonesia, Depok 16424, West Java, IndonesiaDepartment of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia; Research Center of Biomedical Engineering, Universitas Indonesia, Depok 16424, West Java, IndonesiaDepartment of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia; Research Center of Biomedical Engineering, Universitas Indonesia, Depok 16424, West Java, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia; Research Center of Biomedical Engineering, Universitas Indonesia, Depok 16424, West Java, IndonesiaResearch Center for Applied Botany, National Research and Innovation Agency, Cibinong, Bogor 16911, West Java, IndonesiaResearch Center for Applied Botany, National Research and Innovation Agency, Cibinong, Bogor 16911, West Java, IndonesiaResearch Center for Applied Botany, National Research and Innovation Agency, Cibinong, Bogor 16911, West Java, IndonesiaInstitute of Nano Electronic Engineering, Universiti Malaysia Perlis, Seriab 01000 Kangar, Perlis, MalaysiaDetecting Ganoderma boninense in Indonesia is crucial for effectively controlling and mitigating the spread of basal stem disease in oil palm fields. While there is ongoing development of tolerant plants, no such plant has been successfully created yet. Consequently, researchers are actively studying detection methods for Ganoderma boninense. One established and highly accurate approach is the use of polymerase chain reaction (PCR) techniques for molecular detection. However, this method requires time‐consuming sample preparation, which can pose challenges in plantation settings. To address this problem, a portable lab‐on‐chip device has been introduced. This technology enables easy and automatic DNA retrieval from liquid samples by absorbing lysed DNA using magnetic beads. An efficient mechanism for manipulating the magnetic bead within the semiconductor has been successfully implemented. The extraction process typically takes around 15 minutes using a modified methodology on the chip device approach. The chip facilitates the retrieval of two samples with a capacity of 120 µL for each sample. The PCR method was utilized to validate the equivalence of the lab‐on‐chip device extraction to the standard extraction method. This represents a promising alternative for expedited and simplified detection of Ganoderma boninense in field conditions.https://jurnal.ugm.ac.id/ijbiotech/article/view/83645ganoderma boninenseinfected oil palmslab‐on‐chipmolecular detectionpolymerase chain reaction |
| spellingShingle | Adella Josephin Yudan Whulanza Siti Fauziyah Rahman Kenny Lischer Muhammad Imam Surya Irfan Martiansyah Wiguna Rahman Uda Hashim Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device Indonesian Journal of Biotechnology ganoderma boninense infected oil palms lab‐on‐chip molecular detection polymerase chain reaction |
| title | Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device |
| title_full | Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device |
| title_fullStr | Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device |
| title_full_unstemmed | Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device |
| title_short | Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device |
| title_sort | easy extraction of ganoderma boninense liquid sample using portable on chip device |
| topic | ganoderma boninense infected oil palms lab‐on‐chip molecular detection polymerase chain reaction |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/83645 |
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