Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning
Abstract Background Organophosphorus (OP) compounds have been widely available for decades in agriculture for crop protection and as cheap pest controllers, which increases the rate of exposure and poisoning cases. Using serum cholinesterase as prognostic markers for the acute OP toxicity is controv...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-04-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s43141-020-00024-y |
| _version_ | 1827279479467147264 |
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| author | Shimaa Tallat Rania Hussien Rania Hassan Mohamed Mahmoud B. Abd El Wahab Magdy Mahmoud |
| author_facet | Shimaa Tallat Rania Hussien Rania Hassan Mohamed Mahmoud B. Abd El Wahab Magdy Mahmoud |
| author_sort | Shimaa Tallat |
| collection | DOAJ |
| description | Abstract Background Organophosphorus (OP) compounds have been widely available for decades in agriculture for crop protection and as cheap pest controllers, which increases the rate of exposure and poisoning cases. Using serum cholinesterase as prognostic markers for the acute OP toxicity is controversial; therefore, we aim to find out prognostic biomarkers that best correlate with mortality and outcomes of patients with acute OP toxicity. Levels of serum oxidative stress biomarkers (malondialdehyde (MDA) and total antioxidant capacity (TAC)) and activity of the apoptotic biomarkers (caspase 3 and caspase 9) and pseudo-cholinesterase (p.ChE) were performed. Also, we evaluated the apoptotic capacity through determining the genotoxic effects and chromosomal abnormalities among OP intoxicated patients. Results We found the activity of caspases and serum MDA and TAC were significantly increased after OP poisoning and decreased after the appropriate atropine and oxime treatment course. The ROC curve suggested caspases as mortality and outcome predictive markers for acute OP poisoning patients. However, OP poisoning cases before treatment showed significant DNA damage, and they did not show any chromosomal aberration. Conclusion The mentioned results strongly suggest apoptotic-related markers (caspase 3, caspase 9) as prognostic markers for evaluation of the treatment, outcomes, and mortality rate in the acute OP toxicity patients. |
| first_indexed | 2024-04-24T08:19:38Z |
| format | Article |
| id | doaj.art-c79e3db0ab3e4541af2e3835ee95d028 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:19:38Z |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-c79e3db0ab3e4541af2e3835ee95d0282024-04-17T02:11:32ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202020-04-011811810.1186/s43141-020-00024-yCaspases as prognostic markers and mortality predictors in acute organophosphorus poisoningShimaa Tallat0Rania Hussien1Rania Hassan Mohamed2Mahmoud B. Abd El Wahab3Magdy Mahmoud4Poison Control Center–Ain Shams University Hospitals (PCC-ASU)Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Ain Shams UniversityDepartment of Biochemistry, Faculty of Science, Ain Shams UniversityPoison Control Center–Ain Shams University Hospitals (PCC-ASU)Department of Biochemistry, Faculty of Science, Ain Shams UniversityAbstract Background Organophosphorus (OP) compounds have been widely available for decades in agriculture for crop protection and as cheap pest controllers, which increases the rate of exposure and poisoning cases. Using serum cholinesterase as prognostic markers for the acute OP toxicity is controversial; therefore, we aim to find out prognostic biomarkers that best correlate with mortality and outcomes of patients with acute OP toxicity. Levels of serum oxidative stress biomarkers (malondialdehyde (MDA) and total antioxidant capacity (TAC)) and activity of the apoptotic biomarkers (caspase 3 and caspase 9) and pseudo-cholinesterase (p.ChE) were performed. Also, we evaluated the apoptotic capacity through determining the genotoxic effects and chromosomal abnormalities among OP intoxicated patients. Results We found the activity of caspases and serum MDA and TAC were significantly increased after OP poisoning and decreased after the appropriate atropine and oxime treatment course. The ROC curve suggested caspases as mortality and outcome predictive markers for acute OP poisoning patients. However, OP poisoning cases before treatment showed significant DNA damage, and they did not show any chromosomal aberration. Conclusion The mentioned results strongly suggest apoptotic-related markers (caspase 3, caspase 9) as prognostic markers for evaluation of the treatment, outcomes, and mortality rate in the acute OP toxicity patients.http://link.springer.com/article/10.1186/s43141-020-00024-yAcute organophosphorus poisoningApoptosisOxidative stressCaspasesGenotoxicity |
| spellingShingle | Shimaa Tallat Rania Hussien Rania Hassan Mohamed Mahmoud B. Abd El Wahab Magdy Mahmoud Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning Journal of Genetic Engineering and Biotechnology Acute organophosphorus poisoning Apoptosis Oxidative stress Caspases Genotoxicity |
| title | Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning |
| title_full | Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning |
| title_fullStr | Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning |
| title_full_unstemmed | Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning |
| title_short | Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning |
| title_sort | caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning |
| topic | Acute organophosphorus poisoning Apoptosis Oxidative stress Caspases Genotoxicity |
| url | http://link.springer.com/article/10.1186/s43141-020-00024-y |
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