Molecular Detection of Gamma Coronaviruses in Bird Parks of Iran

Gamma Coronaviruses (GCoVs) are distributed worldwide, affecting a wide range of bird species, the beluga whale, and bottlenose dolphins. Because of the limited proofreading capability in the viral encoded polymerase, they emerge genetically diverse. There has been no molecular surveillance data to...

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Bibliographic Details
Main Authors: Hannaneh Yaghoubi, A. Ghalyanchilangeroudi, Vahid Karimi, S. A. Ghafouri, M. Hashemzadeh, H. Hosseini, M. H. Fallah, F. Sadat Mousavi, H. Najafi
Format: Article
Language:English
Published: Razi Vaccine and Serum Research Institute 2019-12-01
Series:Archives of Razi Institute
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Online Access:https://archrazi.areeo.ac.ir/article_120191_c6fae8a431f025b13db442d5fbc00516.pdf
Description
Summary:Gamma Coronaviruses (GCoVs) are distributed worldwide, affecting a wide range of bird species, the beluga whale, and bottlenose dolphins. Because of the limited proofreading capability in the viral encoded polymerase, they emerge genetically diverse. There has been no molecular surveillance data to describe the epidemiology of GCOVs in avian species. The present study was conducted to detect GCOVs in Tehran birds’ parks, 2015. Cloacal swabs (267 samples) from eight  different bird species ((Chickens (<em>Gallus gallus</em>), Pheasant (<em>Phasianus colchicus</em>), Turkey (<em>Meleagris gallopavo</em>), Partridge (<em>Perdix perdix</em>), Quail (<em>Coturnix coturnix</em>), Duck (Anas platyrhynchos), Goose (Anserini),and  Guinea fowl (Numididae)) were collected, the viral RNA was extracted, the RT-PCR was performed using QIAGEN one step RT-PCR kit and the primers targeting “3'-UTR” and “Nucleocapsid” genes. The detection rate was approximately 8.99%. GCOVs were detected in the chicken, quail, pheasant, turkey, and the partridge with different prevalence rates. Phylogenetic tree based on partial nucleotide sequences of the N gene clustered the samples into two groups. It is the first report of GCOVs in non-commercial birds in Iran.  According to our results, GCOVs are circulating in different avian species, and further studies are needed to isolate these viruses and evaluate their pathogenesis.
ISSN:0365-3439
2008-9872