The Protective Effect of Nutraceuticals on Hepatic Ischemia-Reperfusion Injury in Wistar Rats
Nutraceuticals are bioactive compounds present in foods, utilized to ameliorate health, prevent diseases, and support the proper functioning of the human body. They have gained attention due to their ability to hit multiple targets and act as antioxidants, anti-inflammatory agents, and modulators of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/12/10264 |
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| author | Carlos Andrés Pantanali Vinicius Rocha-Santos Márcia Saldanha Kubrusly Inar Alves Castro Luiz Augusto Carneiro-D’Albuquerque Flávio Henrique Galvão |
| author_facet | Carlos Andrés Pantanali Vinicius Rocha-Santos Márcia Saldanha Kubrusly Inar Alves Castro Luiz Augusto Carneiro-D’Albuquerque Flávio Henrique Galvão |
| author_sort | Carlos Andrés Pantanali |
| collection | DOAJ |
| description | Nutraceuticals are bioactive compounds present in foods, utilized to ameliorate health, prevent diseases, and support the proper functioning of the human body. They have gained attention due to their ability to hit multiple targets and act as antioxidants, anti-inflammatory agents, and modulators of immune response and cell death. Therefore, nutraceuticals are being studied to prevent and treat liver ischemia–reperfusion injury (IRI). This study evaluated the effect of a nutraceutical solution formed by resveratrol, quercetin, omega-3 fatty acid, selenium, ginger, avocado, leucine, and niacin on liver IRI. IRI was performed with 60 min of ischemia and 4 h of reperfusion in male Wistar rats. Afterward, the animals were euthanized to study hepatocellular injury, cytokines, oxidative stress, gene expression of apoptosis-related genes, TNF-α and caspase-3 proteins, and histology. Our results show that the nutraceutical solution was able to decrease apoptosis and histologic injury. The suggested mechanisms of action are a reduction in gene expression and the caspase-3 protein and a reduction in the TNF-α protein in liver tissue. The nutraceutical solution was unable to decrease transaminases and cytokines. These findings suggest that the nutraceuticals used favored the protection of hepatocytes, and their combination represents a promising therapeutic proposal against liver IRI. |
| first_indexed | 2024-03-11T02:20:43Z |
| format | Article |
| id | doaj.art-c7a068c034404c2fa606ac26aebf30dd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T02:20:43Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c7a068c034404c2fa606ac26aebf30dd2023-11-18T10:51:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124121026410.3390/ijms241210264The Protective Effect of Nutraceuticals on Hepatic Ischemia-Reperfusion Injury in Wistar RatsCarlos Andrés Pantanali0Vinicius Rocha-Santos1Márcia Saldanha Kubrusly2Inar Alves Castro3Luiz Augusto Carneiro-D’Albuquerque4Flávio Henrique Galvão5Liver and Gastrointestinal Transplant Division, Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo 05403-900, BrazilLiver and Gastrointestinal Transplant Division, Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo 05403-900, BrazilLiver and Gastrointestinal Transplant Division, Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo 05403-900, BrazilLADAF, Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo 01246-000, BrazilLiver and Gastrointestinal Transplant Division, Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo 05403-900, BrazilLiver and Gastrointestinal Transplant Division, Department of Gastroenterology, University of São Paulo School of Medicine, São Paulo 05403-900, BrazilNutraceuticals are bioactive compounds present in foods, utilized to ameliorate health, prevent diseases, and support the proper functioning of the human body. They have gained attention due to their ability to hit multiple targets and act as antioxidants, anti-inflammatory agents, and modulators of immune response and cell death. Therefore, nutraceuticals are being studied to prevent and treat liver ischemia–reperfusion injury (IRI). This study evaluated the effect of a nutraceutical solution formed by resveratrol, quercetin, omega-3 fatty acid, selenium, ginger, avocado, leucine, and niacin on liver IRI. IRI was performed with 60 min of ischemia and 4 h of reperfusion in male Wistar rats. Afterward, the animals were euthanized to study hepatocellular injury, cytokines, oxidative stress, gene expression of apoptosis-related genes, TNF-α and caspase-3 proteins, and histology. Our results show that the nutraceutical solution was able to decrease apoptosis and histologic injury. The suggested mechanisms of action are a reduction in gene expression and the caspase-3 protein and a reduction in the TNF-α protein in liver tissue. The nutraceutical solution was unable to decrease transaminases and cytokines. These findings suggest that the nutraceuticals used favored the protection of hepatocytes, and their combination represents a promising therapeutic proposal against liver IRI.https://www.mdpi.com/1422-0067/24/12/10264nutraceuticalsliverischemia–reperfusion injuryapoptosis |
| spellingShingle | Carlos Andrés Pantanali Vinicius Rocha-Santos Márcia Saldanha Kubrusly Inar Alves Castro Luiz Augusto Carneiro-D’Albuquerque Flávio Henrique Galvão The Protective Effect of Nutraceuticals on Hepatic Ischemia-Reperfusion Injury in Wistar Rats International Journal of Molecular Sciences nutraceuticals liver ischemia–reperfusion injury apoptosis |
| title | The Protective Effect of Nutraceuticals on Hepatic Ischemia-Reperfusion Injury in Wistar Rats |
| title_full | The Protective Effect of Nutraceuticals on Hepatic Ischemia-Reperfusion Injury in Wistar Rats |
| title_fullStr | The Protective Effect of Nutraceuticals on Hepatic Ischemia-Reperfusion Injury in Wistar Rats |
| title_full_unstemmed | The Protective Effect of Nutraceuticals on Hepatic Ischemia-Reperfusion Injury in Wistar Rats |
| title_short | The Protective Effect of Nutraceuticals on Hepatic Ischemia-Reperfusion Injury in Wistar Rats |
| title_sort | protective effect of nutraceuticals on hepatic ischemia reperfusion injury in wistar rats |
| topic | nutraceuticals liver ischemia–reperfusion injury apoptosis |
| url | https://www.mdpi.com/1422-0067/24/12/10264 |
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