Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara
The existence of leprosy or kusta or Morbus Hansen or Hansen’s disease has been known for years, including in Indonesia. Starting from the discovery of Mycobacterium leprae isolates from ancient bone (about 1.000 years B.C), the archaeological excavations results in East Nusa Tenggara, interesting q...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Brawijaya
2012-12-01
|
| Series: | Journal of Tropical Life Science |
| Subjects: | |
| Online Access: | http://jtrolis.ub.ac.id/index.php/jtrolis/article/viewFile/70/72 |
| _version_ | 1827802562660663296 |
|---|---|
| author | Dinar Adriaty Ratna Wahyuni Iswahyudi Bimo Aksono Toetik Koesbardiati Indropo Agusni Shinzo Izumi |
| author_facet | Dinar Adriaty Ratna Wahyuni Iswahyudi Bimo Aksono Toetik Koesbardiati Indropo Agusni Shinzo Izumi |
| author_sort | Dinar Adriaty |
| collection | DOAJ |
| description | The existence of leprosy or kusta or Morbus Hansen or Hansen’s disease has been known for years, including in Indonesia. Starting from the discovery of Mycobacterium leprae isolates from ancient bone (about 1.000 years B.C), the archaeological excavations results in East Nusa Tenggara, interesting questions arise about how the development of leprosy in eastern Indonesia is. Biology molecular study would become a powerful tool to investigate the presence of leprosy bacillary whether there are similarities between the genomes of M. leprae isolates in the primeval and the present. PCR examinations were performed on mandibular bone fragments from ancient human who lived 1000 years B.C. discovered in archaeological surveys on the island of Lembata and three leprosy patients from East Nusa Tenggara. The DNA extraction was performed using a kit from Qiagen products and its TTC repeating pattern was seen with the method of direct sequencing. It turned out that the TTC profile obtained from samples of archaeological was as many as 13 copies, while the repetition of TTC in three samples of leprosy patients were 15, 17 and 26 copies. The different number of TTC repetition shows the different isolates of M. leprae between in the ancient times and the present. Further studies are needed to verify the differences in the genome that occur, for example from the study of SNPs (single nucleotide polymorphisms). |
| first_indexed | 2024-03-11T20:40:43Z |
| format | Article |
| id | doaj.art-c7a31eedf56b4896bc8953326d6ff08e |
| institution | Directory Open Access Journal |
| issn | 2087-5517 |
| language | English |
| last_indexed | 2024-03-11T20:40:43Z |
| publishDate | 2012-12-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj.art-c7a31eedf56b4896bc8953326d6ff08e2023-10-02T02:55:32ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172012-12-01238791Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa TenggaraDinar AdriatyRatna WahyuniIswahyudiBimo AksonoToetik KoesbardiatiIndropo AgusniShinzo IzumiThe existence of leprosy or kusta or Morbus Hansen or Hansen’s disease has been known for years, including in Indonesia. Starting from the discovery of Mycobacterium leprae isolates from ancient bone (about 1.000 years B.C), the archaeological excavations results in East Nusa Tenggara, interesting questions arise about how the development of leprosy in eastern Indonesia is. Biology molecular study would become a powerful tool to investigate the presence of leprosy bacillary whether there are similarities between the genomes of M. leprae isolates in the primeval and the present. PCR examinations were performed on mandibular bone fragments from ancient human who lived 1000 years B.C. discovered in archaeological surveys on the island of Lembata and three leprosy patients from East Nusa Tenggara. The DNA extraction was performed using a kit from Qiagen products and its TTC repeating pattern was seen with the method of direct sequencing. It turned out that the TTC profile obtained from samples of archaeological was as many as 13 copies, while the repetition of TTC in three samples of leprosy patients were 15, 17 and 26 copies. The different number of TTC repetition shows the different isolates of M. leprae between in the ancient times and the present. Further studies are needed to verify the differences in the genome that occur, for example from the study of SNPs (single nucleotide polymorphisms).http://jtrolis.ub.ac.id/index.php/jtrolis/article/viewFile/70/72LeprosyTTC repeatM. lepraeArchaeologyEast Nusa Tenggara |
| spellingShingle | Dinar Adriaty Ratna Wahyuni Iswahyudi Bimo Aksono Toetik Koesbardiati Indropo Agusni Shinzo Izumi Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara Journal of Tropical Life Science Leprosy TTC repeat M. leprae Archaeology East Nusa Tenggara |
| title | Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara |
| title_full | Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara |
| title_fullStr | Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara |
| title_full_unstemmed | Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara |
| title_short | Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara |
| title_sort | variation of ttc repeat pattern in the dna of mycobacterium leprae isolates obtained from archeological bones and leprosy patients from east nusa tenggara |
| topic | Leprosy TTC repeat M. leprae Archaeology East Nusa Tenggara |
| url | http://jtrolis.ub.ac.id/index.php/jtrolis/article/viewFile/70/72 |
| work_keys_str_mv | AT dinaradriaty variationofttcrepeatpatterninthednaofmycobacteriumlepraeisolatesobtainedfromarcheologicalbonesandleprosypatientsfromeastnusatenggara AT ratnawahyuni variationofttcrepeatpatterninthednaofmycobacteriumlepraeisolatesobtainedfromarcheologicalbonesandleprosypatientsfromeastnusatenggara AT iswahyudi variationofttcrepeatpatterninthednaofmycobacteriumlepraeisolatesobtainedfromarcheologicalbonesandleprosypatientsfromeastnusatenggara AT bimoaksono variationofttcrepeatpatterninthednaofmycobacteriumlepraeisolatesobtainedfromarcheologicalbonesandleprosypatientsfromeastnusatenggara AT toetikkoesbardiati variationofttcrepeatpatterninthednaofmycobacteriumlepraeisolatesobtainedfromarcheologicalbonesandleprosypatientsfromeastnusatenggara AT indropoagusni variationofttcrepeatpatterninthednaofmycobacteriumlepraeisolatesobtainedfromarcheologicalbonesandleprosypatientsfromeastnusatenggara AT shinzoizumi variationofttcrepeatpatterninthednaofmycobacteriumlepraeisolatesobtainedfromarcheologicalbonesandleprosypatientsfromeastnusatenggara |