Efficacy of Natural and Allopathic Antimicrobial Agents Incorporated onto Guided Tissue Regeneration Membrane Against Periodontal Pathogens: An In vitro Study
Introduction: Periodontal disease is one of the most prevalent afflictions worldwide. It is an infection of the periodontium as a result of subgingival colonization of the specific microbiota, leading to loss of attachment, which requires optimal care for regeneration to its pre-disease state. G...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2017-01-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/9227/23851_CE[Ra]_F(DK)_PF1(NE_DK)_PFA(DK)_PF2(NE_DK).pdf |
| Summary: | Introduction: Periodontal disease is one of the most prevalent
afflictions worldwide. It is an infection of the periodontium as
a result of subgingival colonization of the specific microbiota,
leading to loss of attachment, which requires optimal care
for regeneration to its pre-disease state. Guided Tissue
Regeneration (GTR) is one of the successful treatment modalities
in Periodontal Regenerative Therapy, but is vulnerable to
bacterial colonization. The conflict between usage of classical
antibiotics and plant origin antimicrobial agents has recently
been in the limelight.
Aim: The aim of this study was to assess the in vitro antimicrobial
activity of amoxicillin, metronidazole and green coffee extract
loaded onto GTR membrane against periodonto-pathogens.
Materials and Methods: Pure form of amoxicillin, metronidazole
and green coffee extract were obtained. One percent concentration of each antimicrobial agent was prepared by appropriate
dilution with distilled water. GTR membrane was cut into a
size of 1x0.5 cm under sterile conditions and was coated
with the antimicrobial agents respectively and with distilled
water as the negative control. Antimicrobial activity was
checked against Aggregatibacter actinomycetemcomitans
(A. actinomycetemcomitans) and Porphyromonas gingivalis
(P. gingivalis) using agar disc diffusion method. The statistical
analysis was done using Kruskal Wallis ANOVA and MannWhitney U test.
Results: One percent amoxicillin showed level of significance
(p>0.05) against both A. actinomycetemcomitans and P.
gingivalis. Green coffee extract showed no zone of inhibition
against both the bacterial species.
Conclusion: Loading of commercially available antimicrobial
agents onto GTR membrane can prevent its bacterial
colonization leading to better treatment outcomes for periodontal
regeneration. |
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| ISSN: | 2249-782X 0973-709X |