Research Note: Transcriptomic analysis of LMH cells in response to the overexpression of a hypothetical protein identified in Eimeria tenella SD-01 strain
ABSTRACT: Though genome sequencing of Eimeria tenella predicts more than 8,000 genes, the molecular functions of many proteins remain unknown. In this study, the coding region corresponding to the mature peptide of a hypothetical protein of E. tenella (ETH_00023950) was amplified and expressed in a...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122003984 |
| _version_ | 1797984577520664576 |
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| author | Bing-Jin Mu Yi-Jing Meng Xiao-Xin Liu Lin-Mei Yu Wen-Bin Zheng Shi-Chen Xie Xing-Quan Zhu Qing Liu Wen-Wei Gao |
| author_facet | Bing-Jin Mu Yi-Jing Meng Xiao-Xin Liu Lin-Mei Yu Wen-Bin Zheng Shi-Chen Xie Xing-Quan Zhu Qing Liu Wen-Wei Gao |
| author_sort | Bing-Jin Mu |
| collection | DOAJ |
| description | ABSTRACT: Though genome sequencing of Eimeria tenella predicts more than 8,000 genes, the molecular functions of many proteins remain unknown. In this study, the coding region corresponding to the mature peptide of a hypothetical protein of E. tenella (ETH_00023950) was amplified and expressed in a bacterial system. Following preparation of polyclonal antibody that recognizes ETH_00023950, the expression of ETH_00023950 in merozoites was examined. Meanwhile, we determined the transcriptomic responses of the leghorn male hepatoma (LMH) cells to its expression. Sequencing analysis showed that one single nucleotide polymorphism and one indel of ETH_00023950 of E. tenella SD-01 strain were found compared with that of the UK reference Houghton strain, leading to a frame shift and a premature stop codon. The expression of ETH_00023950 in E. tenella merozoites was confirmed by indirect immunofluorescence and Western blot analysis. Transcriptomic analysis showed that ETH_00023950 altered the expression of 2,680 genes (321 downregulated genes and 2,359 upregulated genes) in LMH cells. The RNA-sequencing data were consistent with the results of the quantitative real-time polymerase chain reaction (qRT-PCR). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that differentially expressed transcripts were significantly related to 8 pathways, including oxidative phosphorylation and TGF-beta signaling pathway. These findings contribute to understanding host-pathogen interaction and secondary bacterial infections related to E. tenella. |
| first_indexed | 2024-04-11T07:04:53Z |
| format | Article |
| id | doaj.art-c7a74054c65e4114aa24b0dace74e378 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-11T07:04:53Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-c7a74054c65e4114aa24b0dace74e3782022-12-22T04:38:32ZengElsevierPoultry Science0032-57912022-11-0110111102109Research Note: Transcriptomic analysis of LMH cells in response to the overexpression of a hypothetical protein identified in Eimeria tenella SD-01 strainBing-Jin Mu0Yi-Jing Meng1Xiao-Xin Liu2Lin-Mei Yu3Wen-Bin Zheng4Shi-Chen Xie5Xing-Quan Zhu6Qing Liu7Wen-Wei Gao8College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China; Key Laboratory of Veterinary Public Health of Higher Education of Yunnan Province, College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province 650201, PR ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China; Corresponding author:ABSTRACT: Though genome sequencing of Eimeria tenella predicts more than 8,000 genes, the molecular functions of many proteins remain unknown. In this study, the coding region corresponding to the mature peptide of a hypothetical protein of E. tenella (ETH_00023950) was amplified and expressed in a bacterial system. Following preparation of polyclonal antibody that recognizes ETH_00023950, the expression of ETH_00023950 in merozoites was examined. Meanwhile, we determined the transcriptomic responses of the leghorn male hepatoma (LMH) cells to its expression. Sequencing analysis showed that one single nucleotide polymorphism and one indel of ETH_00023950 of E. tenella SD-01 strain were found compared with that of the UK reference Houghton strain, leading to a frame shift and a premature stop codon. The expression of ETH_00023950 in E. tenella merozoites was confirmed by indirect immunofluorescence and Western blot analysis. Transcriptomic analysis showed that ETH_00023950 altered the expression of 2,680 genes (321 downregulated genes and 2,359 upregulated genes) in LMH cells. The RNA-sequencing data were consistent with the results of the quantitative real-time polymerase chain reaction (qRT-PCR). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that differentially expressed transcripts were significantly related to 8 pathways, including oxidative phosphorylation and TGF-beta signaling pathway. These findings contribute to understanding host-pathogen interaction and secondary bacterial infections related to E. tenella.http://www.sciencedirect.com/science/article/pii/S0032579122003984Eimeria tenellaETH_00023950merozoiteexpression analysistranscriptome |
| spellingShingle | Bing-Jin Mu Yi-Jing Meng Xiao-Xin Liu Lin-Mei Yu Wen-Bin Zheng Shi-Chen Xie Xing-Quan Zhu Qing Liu Wen-Wei Gao Research Note: Transcriptomic analysis of LMH cells in response to the overexpression of a hypothetical protein identified in Eimeria tenella SD-01 strain Poultry Science Eimeria tenella ETH_00023950 merozoite expression analysis transcriptome |
| title | Research Note: Transcriptomic analysis of LMH cells in response to the overexpression of a hypothetical protein identified in Eimeria tenella SD-01 strain |
| title_full | Research Note: Transcriptomic analysis of LMH cells in response to the overexpression of a hypothetical protein identified in Eimeria tenella SD-01 strain |
| title_fullStr | Research Note: Transcriptomic analysis of LMH cells in response to the overexpression of a hypothetical protein identified in Eimeria tenella SD-01 strain |
| title_full_unstemmed | Research Note: Transcriptomic analysis of LMH cells in response to the overexpression of a hypothetical protein identified in Eimeria tenella SD-01 strain |
| title_short | Research Note: Transcriptomic analysis of LMH cells in response to the overexpression of a hypothetical protein identified in Eimeria tenella SD-01 strain |
| title_sort | research note transcriptomic analysis of lmh cells in response to the overexpression of a hypothetical protein identified in eimeria tenella sd 01 strain |
| topic | Eimeria tenella ETH_00023950 merozoite expression analysis transcriptome |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122003984 |
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