Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds
Abstract Adenocalymma axillarum (K.Schum.) L.G. Lohmann is a liana belonging to the family Bignoniaceae. In traditional medicine, the genus Adenocalymma is used to treat fever, skin ailments, and body, joint, and facial muscle pains, and it is also applied as cosmetic. Biological assays conducted wi...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2020-11-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100740&lng=en&tlng=en |
| _version_ | 1828130101297938432 |
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| author | João Paulo Campos Andrade Osvaine Junior Alvarenga Alves Marian Caroline Costa Valéria Maria Melleiro Gimenez Iara Silva Squarisi Heloiza Diniz Nicolella Mariana Cintra Pagotti Denise Crispim Tavares Wilson Roberto Cunha Márcio Luís Andrade e Silva Ana Helena Januario Lizandra Guidi Magalhães Patrícia Mendonça Pauletti |
| author_facet | João Paulo Campos Andrade Osvaine Junior Alvarenga Alves Marian Caroline Costa Valéria Maria Melleiro Gimenez Iara Silva Squarisi Heloiza Diniz Nicolella Mariana Cintra Pagotti Denise Crispim Tavares Wilson Roberto Cunha Márcio Luís Andrade e Silva Ana Helena Januario Lizandra Guidi Magalhães Patrícia Mendonça Pauletti |
| author_sort | João Paulo Campos Andrade |
| collection | DOAJ |
| description | Abstract Adenocalymma axillarum (K.Schum.) L.G. Lohmann is a liana belonging to the family Bignoniaceae. In traditional medicine, the genus Adenocalymma is used to treat fever, skin ailments, and body, joint, and facial muscle pains, and it is also applied as cosmetic. Biological assays conducted with the A. axillarum crude leaf ethanol extract have indicated leishmanicidal activity and absence of cytotoxicity. This study aimed to analyze the A. axillarum leaf ethanol crude extract by high-performance liquid chromatography-high-resolution mass spectrometry- diode array detector (HPLC-HRMS-DAD) and to evaluate the leishmanicidal and cytotoxic activities of this crude extract, its fractions, and isolated compounds. HPLC-HRMS-DAD analysis of this extract revealed that it consisted mainly of flavonoids, with nine major compounds. Extract purification yielded 4-hydroxy-N-methylproline, 6-β-hydroxyipolamiide, quercetin-3-O-robinobioside, hyperin, isorhamnetin-3-O-robinobioside, and 3’-O-methylhyperin, which were identified by Nuclear Magnetic Resonance. The isolated compounds were inactive against Leishmania amazonensis promastigotes and human lung fibroblast cells. |
| first_indexed | 2024-04-11T16:29:43Z |
| format | Article |
| id | doaj.art-c7a78c8cb5624d53a2312d1b16a1067b |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-11T16:29:43Z |
| publishDate | 2020-11-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-c7a78c8cb5624d53a2312d1b16a1067b2022-12-22T04:14:05ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902020-11-015810.1590/s2175-97902022e20278Chemical study of Adenocalymma axillarum crude leaf extract and isolated compoundsJoão Paulo Campos Andradehttps://orcid.org/0000-0002-6886-4951Osvaine Junior Alvarenga Alveshttps://orcid.org/0000-0003-1697-9770Marian Caroline Costahttps://orcid.org/0000-0002-6247-1428Valéria Maria Melleiro Gimenezhttps://orcid.org/0000-0002-9995-567XIara Silva Squarisihttps://orcid.org/0000-0003-1962-3702Heloiza Diniz Nicolellahttps://orcid.org/0000-0001-6307-7740Mariana Cintra Pagottihttps://orcid.org/0000-0001-7295-942XDenise Crispim Tavareshttps://orcid.org/0000-0003-4646-5914Wilson Roberto Cunhahttps://orcid.org/0000-0002-1952-1944Márcio Luís Andrade e Silvahttps://orcid.org/0000-0002-9442-4757Ana Helena Januariohttps://orcid.org/0000-0002-3815-6903Lizandra Guidi Magalhãeshttps://orcid.org/0000-0003-2959-6752Patrícia Mendonça Paulettihttps://orcid.org/0000-0003-1831-0050Abstract Adenocalymma axillarum (K.Schum.) L.G. Lohmann is a liana belonging to the family Bignoniaceae. In traditional medicine, the genus Adenocalymma is used to treat fever, skin ailments, and body, joint, and facial muscle pains, and it is also applied as cosmetic. Biological assays conducted with the A. axillarum crude leaf ethanol extract have indicated leishmanicidal activity and absence of cytotoxicity. This study aimed to analyze the A. axillarum leaf ethanol crude extract by high-performance liquid chromatography-high-resolution mass spectrometry- diode array detector (HPLC-HRMS-DAD) and to evaluate the leishmanicidal and cytotoxic activities of this crude extract, its fractions, and isolated compounds. HPLC-HRMS-DAD analysis of this extract revealed that it consisted mainly of flavonoids, with nine major compounds. Extract purification yielded 4-hydroxy-N-methylproline, 6-β-hydroxyipolamiide, quercetin-3-O-robinobioside, hyperin, isorhamnetin-3-O-robinobioside, and 3’-O-methylhyperin, which were identified by Nuclear Magnetic Resonance. The isolated compounds were inactive against Leishmania amazonensis promastigotes and human lung fibroblast cells.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100740&lng=en&tlng=enCytotoxicityFlavonolHPLC-HRMS-DADIridoidLeishmanicidal |
| spellingShingle | João Paulo Campos Andrade Osvaine Junior Alvarenga Alves Marian Caroline Costa Valéria Maria Melleiro Gimenez Iara Silva Squarisi Heloiza Diniz Nicolella Mariana Cintra Pagotti Denise Crispim Tavares Wilson Roberto Cunha Márcio Luís Andrade e Silva Ana Helena Januario Lizandra Guidi Magalhães Patrícia Mendonça Pauletti Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds Brazilian Journal of Pharmaceutical Sciences Cytotoxicity Flavonol HPLC-HRMS-DAD Iridoid Leishmanicidal |
| title | Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds |
| title_full | Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds |
| title_fullStr | Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds |
| title_full_unstemmed | Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds |
| title_short | Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds |
| title_sort | chemical study of adenocalymma axillarum crude leaf extract and isolated compounds |
| topic | Cytotoxicity Flavonol HPLC-HRMS-DAD Iridoid Leishmanicidal |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100740&lng=en&tlng=en |
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