Investigation of Chemopreventive and Antiproliferative Potential of
Context: Carcinogenesis causes much human misery. It is a process involving multistage alterations. Medicinal plants are candidates for beneficial anticancer agents. Objectives: Investigation of anticancer proficiencies of the plant Dicliptera roxburghiana . Material and methods: Crude extract and d...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2022-01-01
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| Series: | Integrative Cancer Therapies |
| Online Access: | https://doi.org/10.1177/15347354211069934 |
| _version_ | 1818892047482880000 |
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| author | Bushra Ahmad PhD Muhammad Rashid Khan PhD Naseer Ali Shah PhD Tamara P. Kondratyuk PhD Nadeem Ahmed PhD John M. Pezzuto PhD Asma Gul PhD Aishma Khattak PhD |
| author_facet | Bushra Ahmad PhD Muhammad Rashid Khan PhD Naseer Ali Shah PhD Tamara P. Kondratyuk PhD Nadeem Ahmed PhD John M. Pezzuto PhD Asma Gul PhD Aishma Khattak PhD |
| author_sort | Bushra Ahmad PhD |
| collection | DOAJ |
| description | Context: Carcinogenesis causes much human misery. It is a process involving multistage alterations. Medicinal plants are candidates for beneficial anticancer agents. Objectives: Investigation of anticancer proficiencies of the plant Dicliptera roxburghiana . Material and methods: Crude extract and derived fractions were inspected for their inhibitory potential against nuclear factor KB (NFκB), nitric oxide synthase inhibition, aromatase inhibition and induction of quinone reductase 1 (QR 1). Antiproliferative activity was determined by using various cancer cell lines for example hormone responsive breast cancer cell line MCF-7, estrogen receptor negative breast cancer cell line MDA-MB-231, murine hepatoma cells Hepa 1c1c7, human neuroblastoma cells SK-N-SH and neuroblastoma cells MYCN-2. Results: Ethyl acetate and n -butanol fractions of D. roxburghiana were strongly active against NFκB with IC 50 of 16.6 ± 1.3 and 8.4 ± 0.7 µg/ml respectively with 100% survival. Chloroform fraction of the plant exhibited an induction ratio of 2.4 ± 0.09 with CD value of 17.7 µg/ml. Regarding the nitrite assay, the n -hexane fraction exhibited significant inhibition of NO activity with IC 50 of 17.8 ± 1.25 µg/ml. The n -butanol fraction exhibited strong antiproliferative activity against IcIc-7 cell lines with IC 50 values of 13.6 ± 1.91 µg/ml; against MYCN-2 a cytotoxic effect developed with dose dependence, with IC 50 of 12.6 ± 1.24 µg/ml. In antiproliferative activity against SK-N-SH cell lines, chloroform, ethyl acetate and n -butanol fractions were efficiently active with IC 50 values of 11.2 ± 0.84, 14.6 ± 1.71 and 16.3 ± 1.57 respectively. Discussion and Conclusion: It was demonstrated that various fractions of D. roxburghiana displayed appreciable anticancer characteristics and could be a potent source for the development of anticancer leads. |
| first_indexed | 2024-12-19T17:50:29Z |
| format | Article |
| id | doaj.art-c7a8d61c41de41658c628a684835d498 |
| institution | Directory Open Access Journal |
| issn | 1552-695X |
| language | English |
| last_indexed | 2024-12-19T17:50:29Z |
| publishDate | 2022-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Integrative Cancer Therapies |
| spelling | doaj.art-c7a8d61c41de41658c628a684835d4982022-12-21T20:11:57ZengSAGE PublishingIntegrative Cancer Therapies1552-695X2022-01-012110.1177/15347354211069934Investigation of Chemopreventive and Antiproliferative Potential ofBushra Ahmad PhD0Muhammad Rashid Khan PhD1Naseer Ali Shah PhD2Tamara P. Kondratyuk PhD3Nadeem Ahmed PhD4John M. Pezzuto PhD5Asma Gul PhD6Aishma Khattak PhD7Shaheed Benazir Bhutto Women University, Peshawar, PakistanQuaid-i-Azam University, Islamabad, PakistanCOMSATS Institute of Information Technology, Islamabad, PakistanUniversity of Hawaii at Hilo, Hilo, HI, USAQuaid-i-Azam University, Islamabad, PakistanUniversity of Hawaii at Hilo, Hilo, HI, USAShaheed Benazir Bhutto Women University, Peshawar, PakistanShaheed Benazir Bhutto Women University, Peshawar, PakistanContext: Carcinogenesis causes much human misery. It is a process involving multistage alterations. Medicinal plants are candidates for beneficial anticancer agents. Objectives: Investigation of anticancer proficiencies of the plant Dicliptera roxburghiana . Material and methods: Crude extract and derived fractions were inspected for their inhibitory potential against nuclear factor KB (NFκB), nitric oxide synthase inhibition, aromatase inhibition and induction of quinone reductase 1 (QR 1). Antiproliferative activity was determined by using various cancer cell lines for example hormone responsive breast cancer cell line MCF-7, estrogen receptor negative breast cancer cell line MDA-MB-231, murine hepatoma cells Hepa 1c1c7, human neuroblastoma cells SK-N-SH and neuroblastoma cells MYCN-2. Results: Ethyl acetate and n -butanol fractions of D. roxburghiana were strongly active against NFκB with IC 50 of 16.6 ± 1.3 and 8.4 ± 0.7 µg/ml respectively with 100% survival. Chloroform fraction of the plant exhibited an induction ratio of 2.4 ± 0.09 with CD value of 17.7 µg/ml. Regarding the nitrite assay, the n -hexane fraction exhibited significant inhibition of NO activity with IC 50 of 17.8 ± 1.25 µg/ml. The n -butanol fraction exhibited strong antiproliferative activity against IcIc-7 cell lines with IC 50 values of 13.6 ± 1.91 µg/ml; against MYCN-2 a cytotoxic effect developed with dose dependence, with IC 50 of 12.6 ± 1.24 µg/ml. In antiproliferative activity against SK-N-SH cell lines, chloroform, ethyl acetate and n -butanol fractions were efficiently active with IC 50 values of 11.2 ± 0.84, 14.6 ± 1.71 and 16.3 ± 1.57 respectively. Discussion and Conclusion: It was demonstrated that various fractions of D. roxburghiana displayed appreciable anticancer characteristics and could be a potent source for the development of anticancer leads.https://doi.org/10.1177/15347354211069934 |
| spellingShingle | Bushra Ahmad PhD Muhammad Rashid Khan PhD Naseer Ali Shah PhD Tamara P. Kondratyuk PhD Nadeem Ahmed PhD John M. Pezzuto PhD Asma Gul PhD Aishma Khattak PhD Investigation of Chemopreventive and Antiproliferative Potential of Integrative Cancer Therapies |
| title | Investigation of Chemopreventive and Antiproliferative Potential of |
| title_full | Investigation of Chemopreventive and Antiproliferative Potential of |
| title_fullStr | Investigation of Chemopreventive and Antiproliferative Potential of |
| title_full_unstemmed | Investigation of Chemopreventive and Antiproliferative Potential of |
| title_short | Investigation of Chemopreventive and Antiproliferative Potential of |
| title_sort | investigation of chemopreventive and antiproliferative potential of |
| url | https://doi.org/10.1177/15347354211069934 |
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