The Impact of Kohlrabi Sprouts on Various Thyroid Parameters in Iodine Deficiency- and Sulfadimethoxine-Induced Hypothyroid Rats
Brassica sprouts, as the rich source of dietary glucosinolates, may have a negative effect on thyroid function. In this study, kohlrabi sprouts diet, combined with two models of rat hypothyroidism, was tested. TSH, thyroid hormones and histopathology analysis were completed with the evaluation of im...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/14/2802 |
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| author | Paweł Paśko Krzysztof Okoń Ewelina Prochownik Mirosław Krośniak Renata Francik Jadwiga Kryczyk-Kozioł Marta Grudzińska Małgorzata Tyszka-Czochara Mateusz Malinowski Jakub Sikora Agnieszka Galanty Paweł Zagrodzki |
| author_facet | Paweł Paśko Krzysztof Okoń Ewelina Prochownik Mirosław Krośniak Renata Francik Jadwiga Kryczyk-Kozioł Marta Grudzińska Małgorzata Tyszka-Czochara Mateusz Malinowski Jakub Sikora Agnieszka Galanty Paweł Zagrodzki |
| author_sort | Paweł Paśko |
| collection | DOAJ |
| description | Brassica sprouts, as the rich source of dietary glucosinolates, may have a negative effect on thyroid function. In this study, kohlrabi sprouts diet, combined with two models of rat hypothyroidism, was tested. TSH, thyroid hormones and histopathology analysis were completed with the evaluation of immunological, biochemical, haematological parameters, cytosolic glutathione peroxidase, thioredoxin reductase in the thyroid, and plasma glutathione peroxidase. A thermographic analysis was also adapted to confirm thyroid dysfunction. The levels of TSH, fT3 and fT4, antioxidant enzyme (GPX) as well as histopathology parameters remained unchanged following kohlrabi sprouts ingestion, only TR activity significantly increased in response to the sprouts. In hypothyroid animals, sprouts diet did not prevent thyroid damage. In comparison with the rats with iodine deficiency, kohlrabi sprouts diet decreased TNF-α level. Neither addition of the sprouts to the diet, nor sulfadimethoxine and iodine deficiency, caused negative changes in red blood cell parameters, glucose and uric acid concentrations, or kidney function. However, such a dietary intervention resulted in reduced WBC levels, and adversely interfered with liver function in rats, most likely due to a higher dietary intake of glucosinolates. Moreover, the possible impact of the breed of the rats on the evaluated parameters was indicated. |
| first_indexed | 2024-03-09T13:15:51Z |
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| id | doaj.art-c7aa6200e7474caa93e9188a592575b4 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T13:15:51Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-c7aa6200e7474caa93e9188a592575b42023-11-30T21:37:02ZengMDPI AGNutrients2072-66432022-07-011414280210.3390/nu14142802The Impact of Kohlrabi Sprouts on Various Thyroid Parameters in Iodine Deficiency- and Sulfadimethoxine-Induced Hypothyroid RatsPaweł Paśko0Krzysztof Okoń1Ewelina Prochownik2Mirosław Krośniak3Renata Francik4Jadwiga Kryczyk-Kozioł5Marta Grudzińska6Małgorzata Tyszka-Czochara7Mateusz Malinowski8Jakub Sikora9Agnieszka Galanty10Paweł Zagrodzki11Department of Food Chemistry and Nutrition, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, PolandDepartment of Pathomorphology, Jagiellonian University Medical College, Grzegórzecka 16, 31-531 Kraków, PolandDepartment of Food Chemistry and Nutrition, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, PolandDepartment of Food Chemistry and Nutrition, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, PolandDepartment of Bioorganic Chemistry, Medical College, Jagiellonian University, Medyczna 9, 30-688 Kraków, PolandDepartment of Food Chemistry and Nutrition, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, PolandDepartment of Pharmacognosy, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, PolandDepartment of Food Chemistry and Nutrition, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, PolandDepartment of Bioprocesses Engineering, Energetics and Automatization, Faculty of Production and Power Engineering, University of Agriculture in Krakow, Balicka 116b, 30-149 Kraków, PolandDepartment of Bioprocesses Engineering, Energetics and Automatization, Faculty of Production and Power Engineering, University of Agriculture in Krakow, Balicka 116b, 30-149 Kraków, PolandDepartment of Pharmacognosy, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, PolandDepartment of Food Chemistry and Nutrition, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, PolandBrassica sprouts, as the rich source of dietary glucosinolates, may have a negative effect on thyroid function. In this study, kohlrabi sprouts diet, combined with two models of rat hypothyroidism, was tested. TSH, thyroid hormones and histopathology analysis were completed with the evaluation of immunological, biochemical, haematological parameters, cytosolic glutathione peroxidase, thioredoxin reductase in the thyroid, and plasma glutathione peroxidase. A thermographic analysis was also adapted to confirm thyroid dysfunction. The levels of TSH, fT3 and fT4, antioxidant enzyme (GPX) as well as histopathology parameters remained unchanged following kohlrabi sprouts ingestion, only TR activity significantly increased in response to the sprouts. In hypothyroid animals, sprouts diet did not prevent thyroid damage. In comparison with the rats with iodine deficiency, kohlrabi sprouts diet decreased TNF-α level. Neither addition of the sprouts to the diet, nor sulfadimethoxine and iodine deficiency, caused negative changes in red blood cell parameters, glucose and uric acid concentrations, or kidney function. However, such a dietary intervention resulted in reduced WBC levels, and adversely interfered with liver function in rats, most likely due to a higher dietary intake of glucosinolates. Moreover, the possible impact of the breed of the rats on the evaluated parameters was indicated.https://www.mdpi.com/2072-6643/14/14/2802kohlrabi sproutsthyroidhistopathologythyroid hormoneTSHiodine deficiency |
| spellingShingle | Paweł Paśko Krzysztof Okoń Ewelina Prochownik Mirosław Krośniak Renata Francik Jadwiga Kryczyk-Kozioł Marta Grudzińska Małgorzata Tyszka-Czochara Mateusz Malinowski Jakub Sikora Agnieszka Galanty Paweł Zagrodzki The Impact of Kohlrabi Sprouts on Various Thyroid Parameters in Iodine Deficiency- and Sulfadimethoxine-Induced Hypothyroid Rats Nutrients kohlrabi sprouts thyroid histopathology thyroid hormone TSH iodine deficiency |
| title | The Impact of Kohlrabi Sprouts on Various Thyroid Parameters in Iodine Deficiency- and Sulfadimethoxine-Induced Hypothyroid Rats |
| title_full | The Impact of Kohlrabi Sprouts on Various Thyroid Parameters in Iodine Deficiency- and Sulfadimethoxine-Induced Hypothyroid Rats |
| title_fullStr | The Impact of Kohlrabi Sprouts on Various Thyroid Parameters in Iodine Deficiency- and Sulfadimethoxine-Induced Hypothyroid Rats |
| title_full_unstemmed | The Impact of Kohlrabi Sprouts on Various Thyroid Parameters in Iodine Deficiency- and Sulfadimethoxine-Induced Hypothyroid Rats |
| title_short | The Impact of Kohlrabi Sprouts on Various Thyroid Parameters in Iodine Deficiency- and Sulfadimethoxine-Induced Hypothyroid Rats |
| title_sort | impact of kohlrabi sprouts on various thyroid parameters in iodine deficiency and sulfadimethoxine induced hypothyroid rats |
| topic | kohlrabi sprouts thyroid histopathology thyroid hormone TSH iodine deficiency |
| url | https://www.mdpi.com/2072-6643/14/14/2802 |
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