Comparison of architect assay and elecsys HBs Ag II assay in the quantification of hepatitis B surface antigen in chronic HBV patients
Background. Hepatitis B virus (HBV) infection is a global health concern, with an estimated 296 million chronic carriers worldwide. Quantification of hepatitis B surface antigen (HBsAg) is a crucial tool in HBV disease management, providing valuable insights into disease progression and treatment e...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Amaltea Medical Publishing House
2023-09-01
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| Series: | Romanian Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | https://rjid.com.ro/articles/2023.3/RJID_2023_3_Art-05.pdf |
| _version_ | 1827348795389640704 |
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| author | T. Uma Arasu P. Kanchanadevi A. Shajahan Thirunavukkarasu Dharmalingam Arbind Kumar Choudhary |
| author_facet | T. Uma Arasu P. Kanchanadevi A. Shajahan Thirunavukkarasu Dharmalingam Arbind Kumar Choudhary |
| author_sort | T. Uma Arasu |
| collection | DOAJ |
| description | Background. Hepatitis B virus (HBV) infection is a global health concern, with an estimated 296 million chronic carriers worldwide. Quantification of hepatitis B surface antigen (HBsAg) is a crucial tool in HBV disease management,
providing valuable insights into disease progression and treatment efficacy.
Objective. To evaluate the comparative performance of the Abbott Architect HBsAg QT and Roche Elecsys HBsAg II assays in quantifying hepatitis B surface antigen (HBsAg) levels in patients with chronic hepatitis B (CHB). Methods. This prospective study enrolled 60 confirmed CHB patients between February 2021 and November 2021. HBsAg levels were measured using both assays and subsequently log-transformed (log10 IU/mL) for statistical analysis. Interclass correlation coefficient (ICC), Pearson correlation coefficient, and Bland-Altman analyses were employed to assess assay concordance and systematic bias.
Results. A statistically significant positive correlation was observed between the Elecsys HBsAg II and Architect HBsAg QT assays for both untreated and treatment-receiving CHB patients (ICC values not specified, p <0.001).
Notably, Architect consistently yielded higher HBsAg measurements compared to Elecsys. Conclusion. Both the Elecsys HBsAg II and Architect HBsAg QT assays demonstrate robust correlation in quantifying HBsAg levels in CHB patients. However, the Architect assay exhibits a systematic positive bias relative to Elecsys, which should be considered when interpreting results and making clinical decisions. Both tools provide reliable assessments for HBsAg quantification within CHB management strategies, but it is essential to be aware of potential assay-specific differences. |
| first_indexed | 2024-03-08T00:18:23Z |
| format | Article |
| id | doaj.art-c7adfdf6353b4bf598d80a8f954470ae |
| institution | Directory Open Access Journal |
| issn | 1454-3389 2069-6051 |
| language | English |
| last_indexed | 2024-03-08T00:18:23Z |
| publishDate | 2023-09-01 |
| publisher | Amaltea Medical Publishing House |
| record_format | Article |
| series | Romanian Journal of Infectious Diseases |
| spelling | doaj.art-c7adfdf6353b4bf598d80a8f954470ae2024-02-16T15:16:28ZengAmaltea Medical Publishing HouseRomanian Journal of Infectious Diseases1454-33892069-60512023-09-01263879110.37897/RJID.2023.3.5Comparison of architect assay and elecsys HBs Ag II assay in the quantification of hepatitis B surface antigen in chronic HBV patientsT. Uma Arasu0P. Kanchanadevi1A. Shajahan2Thirunavukkarasu Dharmalingam3Arbind Kumar Choudhary4Department of Microbiology, “Swamy Vivekanandha” Medical College Hospital & Research Institute, Tiruchengode, Tamilnadu, India Department of Microbiology, CSI College of Dental Science & Research, Madurai, Tamilnadu, IndiaMedical Microbiology, Government Erode Medical College And Hospital, Erode, Tamilnadu, IndiaDepartment of Microbiology, Government “Mohan Kumaramangalam” Medical College and Hospitals, Salem, Tamilnadu, IndiaPharmacology, Government Erode Medical College and Hospital, Tamilnadu, IndiaBackground. Hepatitis B virus (HBV) infection is a global health concern, with an estimated 296 million chronic carriers worldwide. Quantification of hepatitis B surface antigen (HBsAg) is a crucial tool in HBV disease management, providing valuable insights into disease progression and treatment efficacy. Objective. To evaluate the comparative performance of the Abbott Architect HBsAg QT and Roche Elecsys HBsAg II assays in quantifying hepatitis B surface antigen (HBsAg) levels in patients with chronic hepatitis B (CHB). Methods. This prospective study enrolled 60 confirmed CHB patients between February 2021 and November 2021. HBsAg levels were measured using both assays and subsequently log-transformed (log10 IU/mL) for statistical analysis. Interclass correlation coefficient (ICC), Pearson correlation coefficient, and Bland-Altman analyses were employed to assess assay concordance and systematic bias. Results. A statistically significant positive correlation was observed between the Elecsys HBsAg II and Architect HBsAg QT assays for both untreated and treatment-receiving CHB patients (ICC values not specified, p <0.001). Notably, Architect consistently yielded higher HBsAg measurements compared to Elecsys. Conclusion. Both the Elecsys HBsAg II and Architect HBsAg QT assays demonstrate robust correlation in quantifying HBsAg levels in CHB patients. However, the Architect assay exhibits a systematic positive bias relative to Elecsys, which should be considered when interpreting results and making clinical decisions. Both tools provide reliable assessments for HBsAg quantification within CHB management strategies, but it is essential to be aware of potential assay-specific differences.https://rjid.com.ro/articles/2023.3/RJID_2023_3_Art-05.pdfhepatitis b virus (hbv)chronic hepatitis b (chb)hepatitis b surface antigen (hbsag)hbsag quantification assaysabbott architect hbsag qtroche elecsys hbsag iiassay comparisoninterclass correlationbland-altman analysis |
| spellingShingle | T. Uma Arasu P. Kanchanadevi A. Shajahan Thirunavukkarasu Dharmalingam Arbind Kumar Choudhary Comparison of architect assay and elecsys HBs Ag II assay in the quantification of hepatitis B surface antigen in chronic HBV patients Romanian Journal of Infectious Diseases hepatitis b virus (hbv) chronic hepatitis b (chb) hepatitis b surface antigen (hbsag) hbsag quantification assays abbott architect hbsag qt roche elecsys hbsag ii assay comparison interclass correlation bland-altman analysis |
| title | Comparison of architect assay and elecsys HBs Ag II assay in the quantification of hepatitis B surface antigen in chronic HBV patients |
| title_full | Comparison of architect assay and elecsys HBs Ag II assay in the quantification of hepatitis B surface antigen in chronic HBV patients |
| title_fullStr | Comparison of architect assay and elecsys HBs Ag II assay in the quantification of hepatitis B surface antigen in chronic HBV patients |
| title_full_unstemmed | Comparison of architect assay and elecsys HBs Ag II assay in the quantification of hepatitis B surface antigen in chronic HBV patients |
| title_short | Comparison of architect assay and elecsys HBs Ag II assay in the quantification of hepatitis B surface antigen in chronic HBV patients |
| title_sort | comparison of architect assay and elecsys hbs ag ii assay in the quantification of hepatitis b surface antigen in chronic hbv patients |
| topic | hepatitis b virus (hbv) chronic hepatitis b (chb) hepatitis b surface antigen (hbsag) hbsag quantification assays abbott architect hbsag qt roche elecsys hbsag ii assay comparison interclass correlation bland-altman analysis |
| url | https://rjid.com.ro/articles/2023.3/RJID_2023_3_Art-05.pdf |
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