Mast cell stabilizer disodium cromoglycate improves long-term cognitive impairment after general anesthesia exposure in neonatal mice
BackgroundProlonged exposure to general anesthesia (GA) results in long-lasting cognitive impairment, especially during critical stages of brain development. An exaggerated neuroinflammation induced by anesthesia is generally considered to be a key cause of cognitive impairment.Materials and methods...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2022.990333/full |
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| author | Xiaojun Zhang Wensi Wu Zhenzhen Zheng Liang Li Junjun Chen Junying Zhong Le Zhao Jiawei Chen Zhi Wang Fanqing Meng |
| author_facet | Xiaojun Zhang Wensi Wu Zhenzhen Zheng Liang Li Junjun Chen Junying Zhong Le Zhao Jiawei Chen Zhi Wang Fanqing Meng |
| author_sort | Xiaojun Zhang |
| collection | DOAJ |
| description | BackgroundProlonged exposure to general anesthesia (GA) results in long-lasting cognitive impairment, especially during critical stages of brain development. An exaggerated neuroinflammation induced by anesthesia is generally considered to be a key cause of cognitive impairment.Materials and methodsPostnatal day 7 (PND 7) mice were exposed to GA by isoflurane inhalation for 6 h or mock anesthesia. Disodium cromoglycate (DSCG) was intraperitoneally injected daily for 2 weeks, beginning from 30 min before anesthesia. The post-anesthesia evaluation included behavioral tests, toluidine blue staining, immunofluorescence and western blot.ResultsOur results demonstrated the long-term cognition were impaired after 6 h GA exposure in neonatal mice. DSCG treatment ameliorated early mast cells (MCs) degranulation and mast cell tryptase (MCT) expression, which helps to attenuate subsequent neuroinflammation, activation of microglia and astrocytes, and damage to oligodendrocytes and synapses to improve cognitive impairment.ConclusionDisodium cromoglycate could effectively improve long-term cognitive impairment after GA exposure in neonatal mice. |
| first_indexed | 2024-04-12T19:13:59Z |
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| id | doaj.art-c7ae7ef676934c6a9c9281e122c1a446 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-12T19:13:59Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-c7ae7ef676934c6a9c9281e122c1a4462022-12-22T03:19:48ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-09-011610.3389/fnins.2022.990333990333Mast cell stabilizer disodium cromoglycate improves long-term cognitive impairment after general anesthesia exposure in neonatal miceXiaojun Zhang0Wensi Wu1Zhenzhen Zheng2Liang Li3Junjun Chen4Junying Zhong5Le Zhao6Jiawei Chen7Zhi Wang8Fanqing Meng9Department of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, ChinaDepartment of Thoracic Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaDepartment of Anesthesiology, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaDepartment of Anesthesiology, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaDepartment of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, ChinaDepartment of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, ChinaDepartment of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, ChinaDepartment of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, ChinaDepartment of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, ChinaDepartment of Anesthesiology, Jinan Maternity and Child Care Hospital, Jinan, Shandong, ChinaBackgroundProlonged exposure to general anesthesia (GA) results in long-lasting cognitive impairment, especially during critical stages of brain development. An exaggerated neuroinflammation induced by anesthesia is generally considered to be a key cause of cognitive impairment.Materials and methodsPostnatal day 7 (PND 7) mice were exposed to GA by isoflurane inhalation for 6 h or mock anesthesia. Disodium cromoglycate (DSCG) was intraperitoneally injected daily for 2 weeks, beginning from 30 min before anesthesia. The post-anesthesia evaluation included behavioral tests, toluidine blue staining, immunofluorescence and western blot.ResultsOur results demonstrated the long-term cognition were impaired after 6 h GA exposure in neonatal mice. DSCG treatment ameliorated early mast cells (MCs) degranulation and mast cell tryptase (MCT) expression, which helps to attenuate subsequent neuroinflammation, activation of microglia and astrocytes, and damage to oligodendrocytes and synapses to improve cognitive impairment.ConclusionDisodium cromoglycate could effectively improve long-term cognitive impairment after GA exposure in neonatal mice.https://www.frontiersin.org/articles/10.3389/fnins.2022.990333/fullmast celldisodium cromoglycatelong-term cognitive impairmentgeneral anesthesianeonatal mice |
| spellingShingle | Xiaojun Zhang Wensi Wu Zhenzhen Zheng Liang Li Junjun Chen Junying Zhong Le Zhao Jiawei Chen Zhi Wang Fanqing Meng Mast cell stabilizer disodium cromoglycate improves long-term cognitive impairment after general anesthesia exposure in neonatal mice Frontiers in Neuroscience mast cell disodium cromoglycate long-term cognitive impairment general anesthesia neonatal mice |
| title | Mast cell stabilizer disodium cromoglycate improves long-term cognitive impairment after general anesthesia exposure in neonatal mice |
| title_full | Mast cell stabilizer disodium cromoglycate improves long-term cognitive impairment after general anesthesia exposure in neonatal mice |
| title_fullStr | Mast cell stabilizer disodium cromoglycate improves long-term cognitive impairment after general anesthesia exposure in neonatal mice |
| title_full_unstemmed | Mast cell stabilizer disodium cromoglycate improves long-term cognitive impairment after general anesthesia exposure in neonatal mice |
| title_short | Mast cell stabilizer disodium cromoglycate improves long-term cognitive impairment after general anesthesia exposure in neonatal mice |
| title_sort | mast cell stabilizer disodium cromoglycate improves long term cognitive impairment after general anesthesia exposure in neonatal mice |
| topic | mast cell disodium cromoglycate long-term cognitive impairment general anesthesia neonatal mice |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2022.990333/full |
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