Root Metabolism and Effects of Root Exudates on the Growth of <i>Ralstonia solanacearum</i> and <i>Fusarium moniliforme</i> Were Significantly Different between the Two Genotypes of Peanuts
Wild peanut species <i>Arachis correntina</i> (<i>A. correntina</i>) had a higher continuous cropping tolerance than peanut cultivars, closely correlating with the regulatory effects of its root exudates on soil microorganisms. To reveal the resistance mechanism of <i>A...
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MDPI AG
2023-02-01
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| author | Zhong Li Wenfeng Guo Changming Mo Ronghua Tang Liangqiong He Lin Du Ming Li Haining Wu Xiumei Tang Zhipeng Huang Xingjian Wu |
| author_facet | Zhong Li Wenfeng Guo Changming Mo Ronghua Tang Liangqiong He Lin Du Ming Li Haining Wu Xiumei Tang Zhipeng Huang Xingjian Wu |
| author_sort | Zhong Li |
| collection | DOAJ |
| description | Wild peanut species <i>Arachis correntina</i> (<i>A. correntina</i>) had a higher continuous cropping tolerance than peanut cultivars, closely correlating with the regulatory effects of its root exudates on soil microorganisms. To reveal the resistance mechanism of <i>A. correntina</i> to pathogens, we adopted transcriptomic and metabolomics approaches to analyze differentially expressed genes (DEGs) and differentially expressed metabolites (DEMs) between <i>A. correntina</i> and peanut cultivar Guihua85 (GH85) under hydroponic conditions. Interaction experiments of peanut root exudates with <i>Ralstonia solanacearum</i> (<i>R. solanacearum</i>) and <i>Fusarium moniliforme</i> (<i>F. moniliforme</i>) were carried out in this study. The result of transcriptome and metabolomics association analysis showed that there were fewer up-regulated DEGs and DEMs in <i>A. correntina</i> compared with GH85, which were closely associated with the metabolism of amino acids and phenolic acids. Root exudates of GH85 had stronger effects on promoting the growth of <i>R. solanacearum</i> and <i>F. moniliforme</i> than those of <i>A. correntina</i> under 1 and 5 percent volume (1% and 5%) of root exudates treatments. Thirty percent volume (30%) of <i>A. correntina</i> and GH85 root exudates significantly inhibited the growth of two pathogens. The exogenous amino acids and phenolic acids influenced <i>R. solanacearum</i> and <i>F. moniliforme</i> showing concentration effects from growth promotion to inhibition as with the root exudates. In conclusion, the greater resilience of <i>A. correntina</i>) to changes in metabolic pathways for amino acids and phenolic acids might aid in the repression of pathogenic bacteria and fungi. |
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| language | English |
| last_indexed | 2024-03-11T08:47:16Z |
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| spelling | doaj.art-c7b0515094c74ba3b29deb9105ac5eae2023-11-16T20:44:13ZengMDPI AGGenes2073-44252023-02-0114252810.3390/genes14020528Root Metabolism and Effects of Root Exudates on the Growth of <i>Ralstonia solanacearum</i> and <i>Fusarium moniliforme</i> Were Significantly Different between the Two Genotypes of PeanutsZhong Li0Wenfeng Guo1Changming Mo2Ronghua Tang3Liangqiong He4Lin Du5Ming Li6Haining Wu7Xiumei Tang8Zhipeng Huang9Xingjian Wu10Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530004, ChinaGuangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530004, ChinaGuangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530004, ChinaCash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530004, ChinaCash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530004, ChinaGuangxi Science and Technology Museum, Nanning 530016, ChinaGuangxi Science and Technology Museum, Nanning 530016, ChinaCash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530004, ChinaCash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530004, ChinaCash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530004, ChinaGuangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530004, ChinaWild peanut species <i>Arachis correntina</i> (<i>A. correntina</i>) had a higher continuous cropping tolerance than peanut cultivars, closely correlating with the regulatory effects of its root exudates on soil microorganisms. To reveal the resistance mechanism of <i>A. correntina</i> to pathogens, we adopted transcriptomic and metabolomics approaches to analyze differentially expressed genes (DEGs) and differentially expressed metabolites (DEMs) between <i>A. correntina</i> and peanut cultivar Guihua85 (GH85) under hydroponic conditions. Interaction experiments of peanut root exudates with <i>Ralstonia solanacearum</i> (<i>R. solanacearum</i>) and <i>Fusarium moniliforme</i> (<i>F. moniliforme</i>) were carried out in this study. The result of transcriptome and metabolomics association analysis showed that there were fewer up-regulated DEGs and DEMs in <i>A. correntina</i> compared with GH85, which were closely associated with the metabolism of amino acids and phenolic acids. Root exudates of GH85 had stronger effects on promoting the growth of <i>R. solanacearum</i> and <i>F. moniliforme</i> than those of <i>A. correntina</i> under 1 and 5 percent volume (1% and 5%) of root exudates treatments. Thirty percent volume (30%) of <i>A. correntina</i> and GH85 root exudates significantly inhibited the growth of two pathogens. The exogenous amino acids and phenolic acids influenced <i>R. solanacearum</i> and <i>F. moniliforme</i> showing concentration effects from growth promotion to inhibition as with the root exudates. In conclusion, the greater resilience of <i>A. correntina</i>) to changes in metabolic pathways for amino acids and phenolic acids might aid in the repression of pathogenic bacteria and fungi.https://www.mdpi.com/2073-4425/14/2/528peanutroot exudatesdifferentially expressed genesdifferentially expressed metabolitespathogensinteraction |
| spellingShingle | Zhong Li Wenfeng Guo Changming Mo Ronghua Tang Liangqiong He Lin Du Ming Li Haining Wu Xiumei Tang Zhipeng Huang Xingjian Wu Root Metabolism and Effects of Root Exudates on the Growth of <i>Ralstonia solanacearum</i> and <i>Fusarium moniliforme</i> Were Significantly Different between the Two Genotypes of Peanuts Genes peanut root exudates differentially expressed genes differentially expressed metabolites pathogens interaction |
| title | Root Metabolism and Effects of Root Exudates on the Growth of <i>Ralstonia solanacearum</i> and <i>Fusarium moniliforme</i> Were Significantly Different between the Two Genotypes of Peanuts |
| title_full | Root Metabolism and Effects of Root Exudates on the Growth of <i>Ralstonia solanacearum</i> and <i>Fusarium moniliforme</i> Were Significantly Different between the Two Genotypes of Peanuts |
| title_fullStr | Root Metabolism and Effects of Root Exudates on the Growth of <i>Ralstonia solanacearum</i> and <i>Fusarium moniliforme</i> Were Significantly Different between the Two Genotypes of Peanuts |
| title_full_unstemmed | Root Metabolism and Effects of Root Exudates on the Growth of <i>Ralstonia solanacearum</i> and <i>Fusarium moniliforme</i> Were Significantly Different between the Two Genotypes of Peanuts |
| title_short | Root Metabolism and Effects of Root Exudates on the Growth of <i>Ralstonia solanacearum</i> and <i>Fusarium moniliforme</i> Were Significantly Different between the Two Genotypes of Peanuts |
| title_sort | root metabolism and effects of root exudates on the growth of i ralstonia solanacearum i and i fusarium moniliforme i were significantly different between the two genotypes of peanuts |
| topic | peanut root exudates differentially expressed genes differentially expressed metabolites pathogens interaction |
| url | https://www.mdpi.com/2073-4425/14/2/528 |
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