Techniques to Study Antigen-Specific B Cell Responses
Antibodies against foreign antigens are a critical component of the overall immune response and can facilitate pathogen clearance during a primary infection and also protect against subsequent infections. Dysregulation of the antibody response can lead to an autoimmune disease, malignancy, or enhanc...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-07-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.01694/full |
| _version_ | 1818478803691765760 |
|---|---|
| author | Jim Boonyaratanakornkit Justin J. Taylor |
| author_facet | Jim Boonyaratanakornkit Justin J. Taylor |
| author_sort | Jim Boonyaratanakornkit |
| collection | DOAJ |
| description | Antibodies against foreign antigens are a critical component of the overall immune response and can facilitate pathogen clearance during a primary infection and also protect against subsequent infections. Dysregulation of the antibody response can lead to an autoimmune disease, malignancy, or enhanced infection. Since the experimental delineation of a distinct B cell lineage in 1965, various methods have been developed to understand antigen-specific B cell responses in the context of autoimmune diseases, primary immunodeficiencies, infection, and vaccination. In this review, we summarize the established techniques and discuss new and emerging technologies for probing the B cell response in vitro and in vivo by taking advantage of the specificity of B cell receptor (BCR)-associated and secreted antibodies. These include ELISPOT, flow cytometry, mass cytometry, and fluorescence microscopy to identify and/or isolate primary antigen-specific B cells. We also present our approach to identify rare antigen-specific B cells using magnetic enrichment followed by flow cytometry. Once these cells are isolated, in vitro proliferation assays and adoptive transfer experiments in mice can be used to further characterize antigen-specific B cell activation, function, and fate. Transgenic mouse models of B cells targeting model antigens and of B cell signaling have also significantly advanced our understanding of antigen-specific B cell responses in vivo. |
| first_indexed | 2024-12-10T09:52:41Z |
| format | Article |
| id | doaj.art-c7b0773c800c40a4964a429077683a3f |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-10T09:52:41Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-c7b0773c800c40a4964a429077683a3f2022-12-22T01:53:37ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-07-011010.3389/fimmu.2019.01694467535Techniques to Study Antigen-Specific B Cell ResponsesJim BoonyaratanakornkitJustin J. TaylorAntibodies against foreign antigens are a critical component of the overall immune response and can facilitate pathogen clearance during a primary infection and also protect against subsequent infections. Dysregulation of the antibody response can lead to an autoimmune disease, malignancy, or enhanced infection. Since the experimental delineation of a distinct B cell lineage in 1965, various methods have been developed to understand antigen-specific B cell responses in the context of autoimmune diseases, primary immunodeficiencies, infection, and vaccination. In this review, we summarize the established techniques and discuss new and emerging technologies for probing the B cell response in vitro and in vivo by taking advantage of the specificity of B cell receptor (BCR)-associated and secreted antibodies. These include ELISPOT, flow cytometry, mass cytometry, and fluorescence microscopy to identify and/or isolate primary antigen-specific B cells. We also present our approach to identify rare antigen-specific B cells using magnetic enrichment followed by flow cytometry. Once these cells are isolated, in vitro proliferation assays and adoptive transfer experiments in mice can be used to further characterize antigen-specific B cell activation, function, and fate. Transgenic mouse models of B cells targeting model antigens and of B cell signaling have also significantly advanced our understanding of antigen-specific B cell responses in vivo.https://www.frontiersin.org/article/10.3389/fimmu.2019.01694/fullB cellsB lymphocyte subsetshumoral immune responseantigensvaccines |
| spellingShingle | Jim Boonyaratanakornkit Justin J. Taylor Techniques to Study Antigen-Specific B Cell Responses Frontiers in Immunology B cells B lymphocyte subsets humoral immune response antigens vaccines |
| title | Techniques to Study Antigen-Specific B Cell Responses |
| title_full | Techniques to Study Antigen-Specific B Cell Responses |
| title_fullStr | Techniques to Study Antigen-Specific B Cell Responses |
| title_full_unstemmed | Techniques to Study Antigen-Specific B Cell Responses |
| title_short | Techniques to Study Antigen-Specific B Cell Responses |
| title_sort | techniques to study antigen specific b cell responses |
| topic | B cells B lymphocyte subsets humoral immune response antigens vaccines |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.01694/full |
| work_keys_str_mv | AT jimboonyaratanakornkit techniquestostudyantigenspecificbcellresponses AT justinjtaylor techniquestostudyantigenspecificbcellresponses |