YB-1 transferred by gastric cancer exosomes promotes angiogenesis via enhancing the expression of angiogenic factors in vascular endothelial cells
Abstract Background Angiogenesis is important for the progression of gastric cancer (GC). Y-box binding protein 1 (YB-1) predicts advanced disease and indicates neovasculature formation in GC tissues, while the related mechanisms remain elusive. Exosomes mediate intercellular communications via tran...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-10-01
|
| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12885-020-07509-6 |
| _version_ | 1819229146934411264 |
|---|---|
| author | Xiaoxia Xue Jin Huang Kai Yu Xinyue Chen Yini He Dianjun Qi Ying Wu |
| author_facet | Xiaoxia Xue Jin Huang Kai Yu Xinyue Chen Yini He Dianjun Qi Ying Wu |
| author_sort | Xiaoxia Xue |
| collection | DOAJ |
| description | Abstract Background Angiogenesis is important for the progression of gastric cancer (GC). Y-box binding protein 1 (YB-1) predicts advanced disease and indicates neovasculature formation in GC tissues, while the related mechanisms remain elusive. Exosomes mediate intercellular communications via transferring various molecules including proteins, lipids, mRNAs, and microRNAs, while the cargos of GC exosomes and the related mechanisms in GC angiogenesis were rarely reported except for several microRNAs. Methods In this study, human umbilical vein endothelial cells (HUVECs) were, respectively, treated by the exosomes isolated from the YB-1 transfected and the control SGC-7901 cells (SGC-7901-OE-Exo and SGC-7901-NC-Exo), and their apoptosis, proliferation, migration, invasion, and angiogenesis were, sequentially, compared. The levels of angiogenic factors including VEGF, Ang-1, MMP-9 and IL-8 in the exosome-treated HUVECs and the GC-derived exosomes were, separately, detected using PCR and Western blotting as well as RNA sequencing assays. Results We observed the consistent level of YB-1 in the exosomes and their originated GC cells, and the internalization of exosomes into HUVECs. Comparing with SGC-7901-NC-Exo, SGC-7901-OE-Exo significantly inhibited the apoptosis but promoted the proliferation, migration, invasion, and angiogenesis of HUVECs, within which the increased mRNA and protein levels of VEGF, Ang-1, MMP-9 and IL-8 were demonstrated. Meanwhile, mRNA levels of VEGF, Ang-1, MMP-9 and IL-8 showed no significant difference between SGC-7901-NC-Exo and SGC-7901-OE-Exo, although statistically higher mRNA of YB-1 was detected in the SGC-7901-OE-Exo. Conclusions Our findings illustrate YB-1 as the key component of exosome to promote GC angiogenesis by upregulating specific angiogenic factors in the exosome-treated endothelial cells but not in the exosomes themselves. |
| first_indexed | 2024-12-23T11:08:33Z |
| format | Article |
| id | doaj.art-c7b3ecfa1aa44b1098a905353aeb9940 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-23T11:08:33Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-c7b3ecfa1aa44b1098a905353aeb99402022-12-21T17:49:25ZengBMCBMC Cancer1471-24072020-10-0120111210.1186/s12885-020-07509-6YB-1 transferred by gastric cancer exosomes promotes angiogenesis via enhancing the expression of angiogenic factors in vascular endothelial cellsXiaoxia Xue0Jin Huang1Kai Yu2Xinyue Chen3Yini He4Dianjun Qi5Ying Wu6Science Experiment Center, China Medical UniversityDepartment of Radiation Therapy, The First Hospital, China Medical UniversityDepartment of General Practice, The First Hospital, China Medical UniversityDepartment of General Practice, The First Hospital, China Medical UniversityDepartment of General Practice, The First Hospital, China Medical UniversityDepartment of General Practice, The First Hospital, China Medical UniversityDepartment of General Practice, The First Hospital, China Medical UniversityAbstract Background Angiogenesis is important for the progression of gastric cancer (GC). Y-box binding protein 1 (YB-1) predicts advanced disease and indicates neovasculature formation in GC tissues, while the related mechanisms remain elusive. Exosomes mediate intercellular communications via transferring various molecules including proteins, lipids, mRNAs, and microRNAs, while the cargos of GC exosomes and the related mechanisms in GC angiogenesis were rarely reported except for several microRNAs. Methods In this study, human umbilical vein endothelial cells (HUVECs) were, respectively, treated by the exosomes isolated from the YB-1 transfected and the control SGC-7901 cells (SGC-7901-OE-Exo and SGC-7901-NC-Exo), and their apoptosis, proliferation, migration, invasion, and angiogenesis were, sequentially, compared. The levels of angiogenic factors including VEGF, Ang-1, MMP-9 and IL-8 in the exosome-treated HUVECs and the GC-derived exosomes were, separately, detected using PCR and Western blotting as well as RNA sequencing assays. Results We observed the consistent level of YB-1 in the exosomes and their originated GC cells, and the internalization of exosomes into HUVECs. Comparing with SGC-7901-NC-Exo, SGC-7901-OE-Exo significantly inhibited the apoptosis but promoted the proliferation, migration, invasion, and angiogenesis of HUVECs, within which the increased mRNA and protein levels of VEGF, Ang-1, MMP-9 and IL-8 were demonstrated. Meanwhile, mRNA levels of VEGF, Ang-1, MMP-9 and IL-8 showed no significant difference between SGC-7901-NC-Exo and SGC-7901-OE-Exo, although statistically higher mRNA of YB-1 was detected in the SGC-7901-OE-Exo. Conclusions Our findings illustrate YB-1 as the key component of exosome to promote GC angiogenesis by upregulating specific angiogenic factors in the exosome-treated endothelial cells but not in the exosomes themselves.http://link.springer.com/article/10.1186/s12885-020-07509-6YB-1ExosomeGastric cancerAngiogenesisIL-8VEGF |
| spellingShingle | Xiaoxia Xue Jin Huang Kai Yu Xinyue Chen Yini He Dianjun Qi Ying Wu YB-1 transferred by gastric cancer exosomes promotes angiogenesis via enhancing the expression of angiogenic factors in vascular endothelial cells BMC Cancer YB-1 Exosome Gastric cancer Angiogenesis IL-8 VEGF |
| title | YB-1 transferred by gastric cancer exosomes promotes angiogenesis via enhancing the expression of angiogenic factors in vascular endothelial cells |
| title_full | YB-1 transferred by gastric cancer exosomes promotes angiogenesis via enhancing the expression of angiogenic factors in vascular endothelial cells |
| title_fullStr | YB-1 transferred by gastric cancer exosomes promotes angiogenesis via enhancing the expression of angiogenic factors in vascular endothelial cells |
| title_full_unstemmed | YB-1 transferred by gastric cancer exosomes promotes angiogenesis via enhancing the expression of angiogenic factors in vascular endothelial cells |
| title_short | YB-1 transferred by gastric cancer exosomes promotes angiogenesis via enhancing the expression of angiogenic factors in vascular endothelial cells |
| title_sort | yb 1 transferred by gastric cancer exosomes promotes angiogenesis via enhancing the expression of angiogenic factors in vascular endothelial cells |
| topic | YB-1 Exosome Gastric cancer Angiogenesis IL-8 VEGF |
| url | http://link.springer.com/article/10.1186/s12885-020-07509-6 |
| work_keys_str_mv | AT xiaoxiaxue yb1transferredbygastriccancerexosomespromotesangiogenesisviaenhancingtheexpressionofangiogenicfactorsinvascularendothelialcells AT jinhuang yb1transferredbygastriccancerexosomespromotesangiogenesisviaenhancingtheexpressionofangiogenicfactorsinvascularendothelialcells AT kaiyu yb1transferredbygastriccancerexosomespromotesangiogenesisviaenhancingtheexpressionofangiogenicfactorsinvascularendothelialcells AT xinyuechen yb1transferredbygastriccancerexosomespromotesangiogenesisviaenhancingtheexpressionofangiogenicfactorsinvascularendothelialcells AT yinihe yb1transferredbygastriccancerexosomespromotesangiogenesisviaenhancingtheexpressionofangiogenicfactorsinvascularendothelialcells AT dianjunqi yb1transferredbygastriccancerexosomespromotesangiogenesisviaenhancingtheexpressionofangiogenicfactorsinvascularendothelialcells AT yingwu yb1transferredbygastriccancerexosomespromotesangiogenesisviaenhancingtheexpressionofangiogenicfactorsinvascularendothelialcells |