High–efficiency generation of induced pluripotent stem cells from human foreskin fibroblast cells using the Sagunja-tang herbal formula
Abstract Background Sagunja-Tang (SGT-4) is a traditional herbal formula in Korean medicine that is used to treat anti-metabolic syndrome, and has antioxidant activity. In this study, we evaluated the effects of SGT-4 on the formation efficiency of induced pluripotent stem cells (iPSCs) from human f...
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-017-2043-2 |
| _version_ | 1818291232643743744 |
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| author | Ki Mo Kim Deok Rim Heo Joo Young Lee Chang-Seob Seo Sun-Ku Chung |
| author_facet | Ki Mo Kim Deok Rim Heo Joo Young Lee Chang-Seob Seo Sun-Ku Chung |
| author_sort | Ki Mo Kim |
| collection | DOAJ |
| description | Abstract Background Sagunja-Tang (SGT-4) is a traditional herbal formula in Korean medicine that is used to treat anti-metabolic syndrome, and has antioxidant activity. In this study, we evaluated the effects of SGT-4 on the formation efficiency of induced pluripotent stem cells (iPSCs) from human foreskin fibroblasts (HFFs) by four reprogramming transcription factors: Oct4, Sox2, KIf4, and c-Myc (OSKM). Methods SGT-4 contained four different herbal medicines that are composed of Ginseng Radix, Glycyrrhizae Radix et Rhizoma, Atractylodis Rhizoma Alba, and Poria Sclerotium. The composition of SGT-4 was analyzed by high-performance liquid chromatography (HPLC). HFFs were transfected with episomal vectors contained by four OSKM. Western blotting, RT-PCR, immunofluroescence, and in vitro differentiation were used to assess the pluripotency of the iPSC cells. Results SGT-4 exhibited antioxidant activity against the generation of intracellular reactive oxygen species (ROS) as well as promoted the activation of superoxide dismutase 1 (SOD1), catalase, gluthathione peroxidase 1 (GPX1), and glutathione (GSH). Moreover, the ATP level was not significantly fluctuated depending on the concentration of SGT-4 in the hiPSCs. Conclusion Our results indicate that the SGT-4, herbal formula significantly increases the efficiency of human iPSC generation via the transcription factors (Oct4, Sox2, KIf4, and c-Myc). |
| first_indexed | 2024-12-13T02:40:48Z |
| format | Article |
| id | doaj.art-c7b70d34a8f44bc5bc1a64e409468b7e |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-13T02:40:48Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-c7b70d34a8f44bc5bc1a64e409468b7e2022-12-22T00:02:18ZengBMCBMC Complementary and Alternative Medicine1472-68822017-12-011711910.1186/s12906-017-2043-2High–efficiency generation of induced pluripotent stem cells from human foreskin fibroblast cells using the Sagunja-tang herbal formulaKi Mo Kim0Deok Rim Heo1Joo Young Lee2Chang-Seob Seo3Sun-Ku Chung4KM Convergence Research DivisionKM Convergence Research DivisionKM Convergence Research DivisionK-herb Research CenterMibyeong Research Center, Korea Institute of Oriental MedicineAbstract Background Sagunja-Tang (SGT-4) is a traditional herbal formula in Korean medicine that is used to treat anti-metabolic syndrome, and has antioxidant activity. In this study, we evaluated the effects of SGT-4 on the formation efficiency of induced pluripotent stem cells (iPSCs) from human foreskin fibroblasts (HFFs) by four reprogramming transcription factors: Oct4, Sox2, KIf4, and c-Myc (OSKM). Methods SGT-4 contained four different herbal medicines that are composed of Ginseng Radix, Glycyrrhizae Radix et Rhizoma, Atractylodis Rhizoma Alba, and Poria Sclerotium. The composition of SGT-4 was analyzed by high-performance liquid chromatography (HPLC). HFFs were transfected with episomal vectors contained by four OSKM. Western blotting, RT-PCR, immunofluroescence, and in vitro differentiation were used to assess the pluripotency of the iPSC cells. Results SGT-4 exhibited antioxidant activity against the generation of intracellular reactive oxygen species (ROS) as well as promoted the activation of superoxide dismutase 1 (SOD1), catalase, gluthathione peroxidase 1 (GPX1), and glutathione (GSH). Moreover, the ATP level was not significantly fluctuated depending on the concentration of SGT-4 in the hiPSCs. Conclusion Our results indicate that the SGT-4, herbal formula significantly increases the efficiency of human iPSC generation via the transcription factors (Oct4, Sox2, KIf4, and c-Myc).http://link.springer.com/article/10.1186/s12906-017-2043-2Sagunja-tangInduced pluripotent stem cellsReactive oxygen speciesHuman foreskin fibroblastAlkaline phosphatase staining |
| spellingShingle | Ki Mo Kim Deok Rim Heo Joo Young Lee Chang-Seob Seo Sun-Ku Chung High–efficiency generation of induced pluripotent stem cells from human foreskin fibroblast cells using the Sagunja-tang herbal formula BMC Complementary and Alternative Medicine Sagunja-tang Induced pluripotent stem cells Reactive oxygen species Human foreskin fibroblast Alkaline phosphatase staining |
| title | High–efficiency generation of induced pluripotent stem cells from human foreskin fibroblast cells using the Sagunja-tang herbal formula |
| title_full | High–efficiency generation of induced pluripotent stem cells from human foreskin fibroblast cells using the Sagunja-tang herbal formula |
| title_fullStr | High–efficiency generation of induced pluripotent stem cells from human foreskin fibroblast cells using the Sagunja-tang herbal formula |
| title_full_unstemmed | High–efficiency generation of induced pluripotent stem cells from human foreskin fibroblast cells using the Sagunja-tang herbal formula |
| title_short | High–efficiency generation of induced pluripotent stem cells from human foreskin fibroblast cells using the Sagunja-tang herbal formula |
| title_sort | high efficiency generation of induced pluripotent stem cells from human foreskin fibroblast cells using the sagunja tang herbal formula |
| topic | Sagunja-tang Induced pluripotent stem cells Reactive oxygen species Human foreskin fibroblast Alkaline phosphatase staining |
| url | http://link.springer.com/article/10.1186/s12906-017-2043-2 |
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