Isolation, Identification and Production of Encapsulated Bradyrhizobium japonicum and Study on their Viability
Rhizobium, a nitrogen-fixing bacteria is the essential feature of leguminous plants which is essential for the regeneration of nutrient-deficient soil. This study was aimed to isolate, identify, mass culture and immobilize Bradyrhizoium japonicum in encapsulated form and test their viability. Root n...
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| Format: | Article |
| Language: | English |
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Biotechnology Society of Nepal
2019-12-01
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| Series: | Nepal Journal of Biotechnology |
| Subjects: |
| _version_ | 1819143024115974144 |
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| author | Til Kumari Chhetri Bijay Raj Subedee Bijaya Pant |
| author_facet | Til Kumari Chhetri Bijay Raj Subedee Bijaya Pant |
| author_sort | Til Kumari Chhetri |
| collection | DOAJ |
| description | Rhizobium, a nitrogen-fixing bacteria is the essential feature of leguminous plants which is essential for the regeneration of nutrient-deficient soil. This study was aimed to isolate, identify, mass culture and immobilize Bradyrhizoium japonicum in encapsulated form and test their viability. Root nodules were sterilized, grinded and cultured aseptically in YEMA media containing Congo red. The obtained colon was sub-cultured to get a pure culture and different biochemical tests were conducted which proved Bradyrhizobium japonicum as the slow-growing species. The test shows a positive result of catalase production and nodulation test whereas the pH tolerance test shows more tolerance to the acidic pH. Similarly, Bradyrhizaobium japonicum can tolerate 1% and 2% NaCl concentration and it doesn’t show resistance to the penicillin disc of 10mg. The mass culture and encapsulation with sodium alginate adding sucrose as nutrient proved the simplicity for handling. Altogether 548 beads were prepared from the 100ml of the cultured broths which were viable for more than 190 days at 1%, 2% and 3% sucrose concentration but less viable at 5% and 10% sucrose concentration under room temperature. |
| first_indexed | 2024-12-22T12:19:39Z |
| format | Article |
| id | doaj.art-c7b8aa5895144b2c9daa9355963abf63 |
| institution | Directory Open Access Journal |
| issn | 2091-1130 2467-9313 |
| language | English |
| last_indexed | 2024-12-22T12:19:39Z |
| publishDate | 2019-12-01 |
| publisher | Biotechnology Society of Nepal |
| record_format | Article |
| series | Nepal Journal of Biotechnology |
| spelling | doaj.art-c7b8aa5895144b2c9daa9355963abf632022-12-21T18:26:02ZengBiotechnology Society of NepalNepal Journal of Biotechnology2091-11302467-93132019-12-0171394910.3126/njb.v7i1.26950Isolation, Identification and Production of Encapsulated Bradyrhizobium japonicum and Study on their ViabilityTil Kumari Chhetri0Bijay Raj Subedee1Bijaya Pant2Central Department of Botany, Tribhuvan University, Kathmandu, NepalResearch Centre for Applied Science and Technology, Tribhuvan University, Kathmandu, NepalCentral Department of Botany, Tribhuvan University, Kathmandu, NepalRhizobium, a nitrogen-fixing bacteria is the essential feature of leguminous plants which is essential for the regeneration of nutrient-deficient soil. This study was aimed to isolate, identify, mass culture and immobilize Bradyrhizoium japonicum in encapsulated form and test their viability. Root nodules were sterilized, grinded and cultured aseptically in YEMA media containing Congo red. The obtained colon was sub-cultured to get a pure culture and different biochemical tests were conducted which proved Bradyrhizobium japonicum as the slow-growing species. The test shows a positive result of catalase production and nodulation test whereas the pH tolerance test shows more tolerance to the acidic pH. Similarly, Bradyrhizaobium japonicum can tolerate 1% and 2% NaCl concentration and it doesn’t show resistance to the penicillin disc of 10mg. The mass culture and encapsulation with sodium alginate adding sucrose as nutrient proved the simplicity for handling. Altogether 548 beads were prepared from the 100ml of the cultured broths which were viable for more than 190 days at 1%, 2% and 3% sucrose concentration but less viable at 5% and 10% sucrose concentration under room temperature.bradyrhizobiumencapsulationimmobilizationviabiliyylegumessymbiotic bacteria |
| spellingShingle | Til Kumari Chhetri Bijay Raj Subedee Bijaya Pant Isolation, Identification and Production of Encapsulated Bradyrhizobium japonicum and Study on their Viability Nepal Journal of Biotechnology bradyrhizobium encapsulation immobilization viabiliyy legumes symbiotic bacteria |
| title | Isolation, Identification and Production of Encapsulated Bradyrhizobium japonicum and Study on their Viability |
| title_full | Isolation, Identification and Production of Encapsulated Bradyrhizobium japonicum and Study on their Viability |
| title_fullStr | Isolation, Identification and Production of Encapsulated Bradyrhizobium japonicum and Study on their Viability |
| title_full_unstemmed | Isolation, Identification and Production of Encapsulated Bradyrhizobium japonicum and Study on their Viability |
| title_short | Isolation, Identification and Production of Encapsulated Bradyrhizobium japonicum and Study on their Viability |
| title_sort | isolation identification and production of encapsulated bradyrhizobium japonicum and study on their viability |
| topic | bradyrhizobium encapsulation immobilization viabiliyy legumes symbiotic bacteria |
| work_keys_str_mv | AT tilkumarichhetri isolationidentificationandproductionofencapsulatedbradyrhizobiumjaponicumandstudyontheirviability AT bijayrajsubedee isolationidentificationandproductionofencapsulatedbradyrhizobiumjaponicumandstudyontheirviability AT bijayapant isolationidentificationandproductionofencapsulatedbradyrhizobiumjaponicumandstudyontheirviability |