Neuroprotective effects of a novel peptide from Lignosus rhinocerotis against 6‐hydroxydopamine‐induced apoptosis in PC12 cells by inhibiting NF‐κB activation
Abstract According to previous studies, oxidative stress is a leading cause of dopaminergic neuron death and may contribute to the pathogenesis of Parkinson's disease (PD). In the current study, we used chromatography of gel filtration to identify a novel peptide (Lignosus rhinocerotis peptide...
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| Format: | Article |
| Language: | English |
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Wiley
2023-05-01
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| Series: | Food Science & Nutrition |
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| Online Access: | https://doi.org/10.1002/fsn3.3050 |
| _version_ | 1797829714192105472 |
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| author | Chuan Xiong Yu Zhu Qiang Luo Chia Wei Phan Yujie Huo Ping Li Qiang Li Xin Jin Wenli Huang |
| author_facet | Chuan Xiong Yu Zhu Qiang Luo Chia Wei Phan Yujie Huo Ping Li Qiang Li Xin Jin Wenli Huang |
| author_sort | Chuan Xiong |
| collection | DOAJ |
| description | Abstract According to previous studies, oxidative stress is a leading cause of dopaminergic neuron death and may contribute to the pathogenesis of Parkinson's disease (PD). In the current study, we used chromatography of gel filtration to identify a novel peptide (Lignosus rhinocerotis peptide [LRP]) from the sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden. Its neuroprotective effect was evaluated using an in vitro PD model constructed by 6‐hydroxydopamine (6‐OHDA)‐stimulated to apoptosis in PC12 cells. The molecular weight of LRP is determined as 1532 Da and the secondary structure is irregular. The simple amino acid sequence of LRP is Thr‐Leu‐Ala‐Pro‐Thr‐Phe‐Leu‐Ser‐Ser‐Leu‐Gly‐Pro‐Cys‐Leu‐Leu. Notably, LRP has the ability to significantly boost the viability of PC12 cells after exposure to 6‐OHDA, as well as enhance the cellular activity of antioxidative enzymes like superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH‐Px). LRP also lowers the level of malondialdehyde (MDA), decreases the activation performance of Caspase‐3, and reduces 6‐OHDA‐induced apoptosis via inhibition of nuclear factor‐kappa B (NF‐κB) activation. These data indicate that LRP may have the potential to act as a neuroprotective agent. |
| first_indexed | 2024-04-09T13:24:41Z |
| format | Article |
| id | doaj.art-c7b8e453b1b542f186d99d51a56e0e9d |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-04-09T13:24:41Z |
| publishDate | 2023-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-c7b8e453b1b542f186d99d51a56e0e9d2023-05-10T16:39:00ZengWileyFood Science & Nutrition2048-71772023-05-011152152216510.1002/fsn3.3050Neuroprotective effects of a novel peptide from Lignosus rhinocerotis against 6‐hydroxydopamine‐induced apoptosis in PC12 cells by inhibiting NF‐κB activationChuan Xiong0Yu Zhu1Qiang Luo2Chia Wei Phan3Yujie Huo4Ping Li5Qiang Li6Xin Jin7Wenli Huang8Biotechnology and Nuclear Technology Research Institute Sichuan Academy of Agricultural Sciences Chengdu ChinaBiotechnology and Nuclear Technology Research Institute Sichuan Academy of Agricultural Sciences Chengdu ChinaThe Second Affiliated Hospital Chongqing Medical University Chongqing ChinaMushroom Research Centre Universiti Malaya Kuala Lumpur MalaysiaYunnan Plateau Characteristic Agricultural Industry Research Institute Yunnan Agricultural University Kunming ChinaBiotechnology and Nuclear Technology Research Institute Sichuan Academy of Agricultural Sciences Chengdu ChinaCollege of Food and Biological Engineering Chengdu University Chengdu ChinaBiotechnology and Nuclear Technology Research Institute Sichuan Academy of Agricultural Sciences Chengdu ChinaBiotechnology and Nuclear Technology Research Institute Sichuan Academy of Agricultural Sciences Chengdu ChinaAbstract According to previous studies, oxidative stress is a leading cause of dopaminergic neuron death and may contribute to the pathogenesis of Parkinson's disease (PD). In the current study, we used chromatography of gel filtration to identify a novel peptide (Lignosus rhinocerotis peptide [LRP]) from the sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden. Its neuroprotective effect was evaluated using an in vitro PD model constructed by 6‐hydroxydopamine (6‐OHDA)‐stimulated to apoptosis in PC12 cells. The molecular weight of LRP is determined as 1532 Da and the secondary structure is irregular. The simple amino acid sequence of LRP is Thr‐Leu‐Ala‐Pro‐Thr‐Phe‐Leu‐Ser‐Ser‐Leu‐Gly‐Pro‐Cys‐Leu‐Leu. Notably, LRP has the ability to significantly boost the viability of PC12 cells after exposure to 6‐OHDA, as well as enhance the cellular activity of antioxidative enzymes like superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH‐Px). LRP also lowers the level of malondialdehyde (MDA), decreases the activation performance of Caspase‐3, and reduces 6‐OHDA‐induced apoptosis via inhibition of nuclear factor‐kappa B (NF‐κB) activation. These data indicate that LRP may have the potential to act as a neuroprotective agent.https://doi.org/10.1002/fsn3.3050Lignosus rhinocerotisneuroprotective effectoxidative stresspeptide |
| spellingShingle | Chuan Xiong Yu Zhu Qiang Luo Chia Wei Phan Yujie Huo Ping Li Qiang Li Xin Jin Wenli Huang Neuroprotective effects of a novel peptide from Lignosus rhinocerotis against 6‐hydroxydopamine‐induced apoptosis in PC12 cells by inhibiting NF‐κB activation Food Science & Nutrition Lignosus rhinocerotis neuroprotective effect oxidative stress peptide |
| title | Neuroprotective effects of a novel peptide from Lignosus rhinocerotis against 6‐hydroxydopamine‐induced apoptosis in PC12 cells by inhibiting NF‐κB activation |
| title_full | Neuroprotective effects of a novel peptide from Lignosus rhinocerotis against 6‐hydroxydopamine‐induced apoptosis in PC12 cells by inhibiting NF‐κB activation |
| title_fullStr | Neuroprotective effects of a novel peptide from Lignosus rhinocerotis against 6‐hydroxydopamine‐induced apoptosis in PC12 cells by inhibiting NF‐κB activation |
| title_full_unstemmed | Neuroprotective effects of a novel peptide from Lignosus rhinocerotis against 6‐hydroxydopamine‐induced apoptosis in PC12 cells by inhibiting NF‐κB activation |
| title_short | Neuroprotective effects of a novel peptide from Lignosus rhinocerotis against 6‐hydroxydopamine‐induced apoptosis in PC12 cells by inhibiting NF‐κB activation |
| title_sort | neuroprotective effects of a novel peptide from lignosus rhinocerotis against 6 hydroxydopamine induced apoptosis in pc12 cells by inhibiting nf κb activation |
| topic | Lignosus rhinocerotis neuroprotective effect oxidative stress peptide |
| url | https://doi.org/10.1002/fsn3.3050 |
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