Xanthophyll-Rich Extract of <i>Phaeodactylum tricornutum</i> Bohlin as New Photoprotective Cosmeceutical Agent: Safety and Efficacy Assessment on In Vitro Reconstructed Human Epidermis Model
The nutritional and health properties of algae make them perfect functional ingredients for nutraceutical and cosmeceutical applications. In this study, the <i>Phaeodactylum tricornutum</i> Bohlin (Phaeodactylaceae), a pleiomorphic diatom commonly found in marine ecosystems, was investig...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2023-05-01
|
Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/28/10/4190 |
_version_ | 1797598875238793216 |
---|---|
author | Antonella Smeriglio Joseph Lionti Mariarosaria Ingegneri Bruno Burlando Laura Cornara Federica Grillo Luca Mastracci Domenico Trombetta |
author_facet | Antonella Smeriglio Joseph Lionti Mariarosaria Ingegneri Bruno Burlando Laura Cornara Federica Grillo Luca Mastracci Domenico Trombetta |
author_sort | Antonella Smeriglio |
collection | DOAJ |
description | The nutritional and health properties of algae make them perfect functional ingredients for nutraceutical and cosmeceutical applications. In this study, the <i>Phaeodactylum tricornutum</i> Bohlin (Phaeodactylaceae), a pleiomorphic diatom commonly found in marine ecosystems, was investigated. The in vitro culture conditions used favoured the fusiform morphotype, characterized by a high accumulation of neutral lipids, as detected by fluorescence microscopy after BODIPY staining. These data were confirmed by HPLC-DAD-APCI-MS/MS analyses carried out on the ethanolic extract (PTE), which showed a high content of xanthophylls (98.99%), and in particular of fucoxanthin (Fx, 6.67 g/100 g PTE). The antioxidant activity (ORAC, FRAP, TEAC and β-carotene bleaching) and photostability of PTE and Fx against UVA and UVB rays were firstly evaluated by in vitro cell-free assays. After this, phototoxicity and photoprotective studies were carried out on in vitro reconstructed human epidermidis models. Results demonstrated that PTE (0.1% Fx) and 0.1% Fx, both photostable, significantly (<i>p</i> < 0.05) reduce oxidative and inflammatory stress markers (ROS, NO and IL-1α), as well as cytotoxicity and sunburn cells induced by UVA and UVB doses simulating the solar radiation, with an excellent safety profile. However, PTE proved to be more effective than Fx, suggesting its effective and safe use in broad-spectrum sunscreens. |
first_indexed | 2024-03-11T03:27:44Z |
format | Article |
id | doaj.art-c7b95065a67f46f8bf8442d8edd9e2df |
institution | Directory Open Access Journal |
issn | 1420-3049 |
language | English |
last_indexed | 2024-03-11T03:27:44Z |
publishDate | 2023-05-01 |
publisher | MDPI AG |
record_format | Article |
series | Molecules |
spelling | doaj.art-c7b95065a67f46f8bf8442d8edd9e2df2023-11-18T02:40:37ZengMDPI AGMolecules1420-30492023-05-012810419010.3390/molecules28104190Xanthophyll-Rich Extract of <i>Phaeodactylum tricornutum</i> Bohlin as New Photoprotective Cosmeceutical Agent: Safety and Efficacy Assessment on In Vitro Reconstructed Human Epidermis ModelAntonella Smeriglio0Joseph Lionti1Mariarosaria Ingegneri2Bruno Burlando3Laura Cornara4Federica Grillo5Luca Mastracci6Domenico Trombetta7Department of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Viale Ferdinando Stagno d’Alcontres 31, 98166 Messina, ItalyArchimede Ricerche Srl, Corso Italia 220, 18033 Camporosso, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Viale Ferdinando Stagno d’Alcontres 31, 98166 Messina, ItalyDepartment of Pharmacy—DIFAR, University of Genova, Viale Benedetto XV 3, 16132 Genova, ItalyDepartment of Earth, Environment and Life Sciences (DISTAV), University of Genova, Corso Europa 26, 16132 Genova, ItalyPathology Unit, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genova, 16132 Genova, ItalyPathology Unit, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genova, 16132 Genova, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Viale Ferdinando Stagno d’Alcontres 31, 98166 Messina, ItalyThe nutritional and health properties of algae make them perfect functional ingredients for nutraceutical and cosmeceutical applications. In this study, the <i>Phaeodactylum tricornutum</i> Bohlin (Phaeodactylaceae), a pleiomorphic diatom commonly found in marine ecosystems, was investigated. The in vitro culture conditions used favoured the fusiform morphotype, characterized by a high accumulation of neutral lipids, as detected by fluorescence microscopy after BODIPY staining. These data were confirmed by HPLC-DAD-APCI-MS/MS analyses carried out on the ethanolic extract (PTE), which showed a high content of xanthophylls (98.99%), and in particular of fucoxanthin (Fx, 6.67 g/100 g PTE). The antioxidant activity (ORAC, FRAP, TEAC and β-carotene bleaching) and photostability of PTE and Fx against UVA and UVB rays were firstly evaluated by in vitro cell-free assays. After this, phototoxicity and photoprotective studies were carried out on in vitro reconstructed human epidermidis models. Results demonstrated that PTE (0.1% Fx) and 0.1% Fx, both photostable, significantly (<i>p</i> < 0.05) reduce oxidative and inflammatory stress markers (ROS, NO and IL-1α), as well as cytotoxicity and sunburn cells induced by UVA and UVB doses simulating the solar radiation, with an excellent safety profile. However, PTE proved to be more effective than Fx, suggesting its effective and safe use in broad-spectrum sunscreens.https://www.mdpi.com/1420-3049/28/10/4190microalgae<i>Phaeodactylum tricornutum</i> bohlinmicroscopyphytochemical analysiscarotenoidsfucoxanthin |
spellingShingle | Antonella Smeriglio Joseph Lionti Mariarosaria Ingegneri Bruno Burlando Laura Cornara Federica Grillo Luca Mastracci Domenico Trombetta Xanthophyll-Rich Extract of <i>Phaeodactylum tricornutum</i> Bohlin as New Photoprotective Cosmeceutical Agent: Safety and Efficacy Assessment on In Vitro Reconstructed Human Epidermis Model Molecules microalgae <i>Phaeodactylum tricornutum</i> bohlin microscopy phytochemical analysis carotenoids fucoxanthin |
title | Xanthophyll-Rich Extract of <i>Phaeodactylum tricornutum</i> Bohlin as New Photoprotective Cosmeceutical Agent: Safety and Efficacy Assessment on In Vitro Reconstructed Human Epidermis Model |
title_full | Xanthophyll-Rich Extract of <i>Phaeodactylum tricornutum</i> Bohlin as New Photoprotective Cosmeceutical Agent: Safety and Efficacy Assessment on In Vitro Reconstructed Human Epidermis Model |
title_fullStr | Xanthophyll-Rich Extract of <i>Phaeodactylum tricornutum</i> Bohlin as New Photoprotective Cosmeceutical Agent: Safety and Efficacy Assessment on In Vitro Reconstructed Human Epidermis Model |
title_full_unstemmed | Xanthophyll-Rich Extract of <i>Phaeodactylum tricornutum</i> Bohlin as New Photoprotective Cosmeceutical Agent: Safety and Efficacy Assessment on In Vitro Reconstructed Human Epidermis Model |
title_short | Xanthophyll-Rich Extract of <i>Phaeodactylum tricornutum</i> Bohlin as New Photoprotective Cosmeceutical Agent: Safety and Efficacy Assessment on In Vitro Reconstructed Human Epidermis Model |
title_sort | xanthophyll rich extract of i phaeodactylum tricornutum i bohlin as new photoprotective cosmeceutical agent safety and efficacy assessment on in vitro reconstructed human epidermis model |
topic | microalgae <i>Phaeodactylum tricornutum</i> bohlin microscopy phytochemical analysis carotenoids fucoxanthin |
url | https://www.mdpi.com/1420-3049/28/10/4190 |
work_keys_str_mv | AT antonellasmeriglio xanthophyllrichextractofiphaeodactylumtricornutumibohlinasnewphotoprotectivecosmeceuticalagentsafetyandefficacyassessmentoninvitroreconstructedhumanepidermismodel AT josephlionti xanthophyllrichextractofiphaeodactylumtricornutumibohlinasnewphotoprotectivecosmeceuticalagentsafetyandefficacyassessmentoninvitroreconstructedhumanepidermismodel AT mariarosariaingegneri xanthophyllrichextractofiphaeodactylumtricornutumibohlinasnewphotoprotectivecosmeceuticalagentsafetyandefficacyassessmentoninvitroreconstructedhumanepidermismodel AT brunoburlando xanthophyllrichextractofiphaeodactylumtricornutumibohlinasnewphotoprotectivecosmeceuticalagentsafetyandefficacyassessmentoninvitroreconstructedhumanepidermismodel AT lauracornara xanthophyllrichextractofiphaeodactylumtricornutumibohlinasnewphotoprotectivecosmeceuticalagentsafetyandefficacyassessmentoninvitroreconstructedhumanepidermismodel AT federicagrillo xanthophyllrichextractofiphaeodactylumtricornutumibohlinasnewphotoprotectivecosmeceuticalagentsafetyandefficacyassessmentoninvitroreconstructedhumanepidermismodel AT lucamastracci xanthophyllrichextractofiphaeodactylumtricornutumibohlinasnewphotoprotectivecosmeceuticalagentsafetyandefficacyassessmentoninvitroreconstructedhumanepidermismodel AT domenicotrombetta xanthophyllrichextractofiphaeodactylumtricornutumibohlinasnewphotoprotectivecosmeceuticalagentsafetyandefficacyassessmentoninvitroreconstructedhumanepidermismodel |