<it>Burkholderia </it>Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis
<p>Abstract</p> <p>Background</p> <p>The bacterial biothreat agents <it>Burkholderia mallei </it>and <it>Burkholderia pseudomallei </it>are the cause of glanders and melioidosis, respectively. Genomic and epidemiological studies have shown that &...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-03-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/7/19 |
| _version_ | 1818037906239913984 |
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| author | Foster Simon J Clarke Simon R Rengpipat Sirirat Tumapa Sarinna Holden Matthew TG Tiyawisutsri Rachaneeporn Nierman William C Day Nicholas PJ Peacock Sharon J |
| author_facet | Foster Simon J Clarke Simon R Rengpipat Sirirat Tumapa Sarinna Holden Matthew TG Tiyawisutsri Rachaneeporn Nierman William C Day Nicholas PJ Peacock Sharon J |
| author_sort | Foster Simon J |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The bacterial biothreat agents <it>Burkholderia mallei </it>and <it>Burkholderia pseudomallei </it>are the cause of glanders and melioidosis, respectively. Genomic and epidemiological studies have shown that <it>B. mallei </it>is a recently emerged, host restricted clone of <it>B. pseudomallei</it>.</p> <p>Results</p> <p>Using bacteriophage-mediated immunoscreening we identified genes expressed <it>in vivo </it>during experimental equine glanders infection. A family of immunodominant antigens were identified that share protein domain architectures with hemagglutinins and invasins. These have been designated <it>Burkholderia </it>Hep_Hag autotransporter (BuHA) proteins. A total of 110/207 positive clones (53%) of a <it>B. mallei </it>expression library screened with sera from two infected horses belonged to this family. This contrasted with 6/189 positive clones (3%) of a <it>B. pseudomallei </it>expression library screened with serum from 21 patients with culture-proven melioidosis.</p> <p>Conclusion</p> <p>Members of the BuHA proteins are found in other Gram-negative bacteria and have been shown to have important roles related to virulence. Compared with other bacterial species, the genomes of both <it>B. mallei and B. pseudomallei </it>contain a relative abundance of this family of proteins. The domain structures of these proteins suggest that they function as multimeric surface proteins that modulate interactions of the cell with the host and environment. Their effect on the cellular immune response to <it>B. mallei </it>and their potential as diagnostics for glanders requires further study.</p> |
| first_indexed | 2024-12-10T07:34:17Z |
| format | Article |
| id | doaj.art-c7b98100720f451e8c7c6f637e5fc4b5 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-10T07:34:17Z |
| publishDate | 2007-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-c7b98100720f451e8c7c6f637e5fc4b52022-12-22T01:57:28ZengBMCBMC Microbiology1471-21802007-03-01711910.1186/1471-2180-7-19<it>Burkholderia </it>Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosisFoster Simon JClarke Simon RRengpipat SiriratTumapa SarinnaHolden Matthew TGTiyawisutsri RachaneepornNierman William CDay Nicholas PJPeacock Sharon J<p>Abstract</p> <p>Background</p> <p>The bacterial biothreat agents <it>Burkholderia mallei </it>and <it>Burkholderia pseudomallei </it>are the cause of glanders and melioidosis, respectively. Genomic and epidemiological studies have shown that <it>B. mallei </it>is a recently emerged, host restricted clone of <it>B. pseudomallei</it>.</p> <p>Results</p> <p>Using bacteriophage-mediated immunoscreening we identified genes expressed <it>in vivo </it>during experimental equine glanders infection. A family of immunodominant antigens were identified that share protein domain architectures with hemagglutinins and invasins. These have been designated <it>Burkholderia </it>Hep_Hag autotransporter (BuHA) proteins. A total of 110/207 positive clones (53%) of a <it>B. mallei </it>expression library screened with sera from two infected horses belonged to this family. This contrasted with 6/189 positive clones (3%) of a <it>B. pseudomallei </it>expression library screened with serum from 21 patients with culture-proven melioidosis.</p> <p>Conclusion</p> <p>Members of the BuHA proteins are found in other Gram-negative bacteria and have been shown to have important roles related to virulence. Compared with other bacterial species, the genomes of both <it>B. mallei and B. pseudomallei </it>contain a relative abundance of this family of proteins. The domain structures of these proteins suggest that they function as multimeric surface proteins that modulate interactions of the cell with the host and environment. Their effect on the cellular immune response to <it>B. mallei </it>and their potential as diagnostics for glanders requires further study.</p>http://www.biomedcentral.com/1471-2180/7/19 |
| spellingShingle | Foster Simon J Clarke Simon R Rengpipat Sirirat Tumapa Sarinna Holden Matthew TG Tiyawisutsri Rachaneeporn Nierman William C Day Nicholas PJ Peacock Sharon J <it>Burkholderia </it>Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis BMC Microbiology |
| title | <it>Burkholderia </it>Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis |
| title_full | <it>Burkholderia </it>Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis |
| title_fullStr | <it>Burkholderia </it>Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis |
| title_full_unstemmed | <it>Burkholderia </it>Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis |
| title_short | <it>Burkholderia </it>Hep_Hag autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis |
| title_sort | it burkholderia it hep hag autotransporter buha proteins elicit a strong antibody response during experimental glanders but not human melioidosis |
| url | http://www.biomedcentral.com/1471-2180/7/19 |
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