Screening of a food poisoning caused by alkaloids by ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry
ObjectiveScreening bio-samples from a food poisoning event based on ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF MS), and to provide strategies and references for patient treatment as well as the event response.MethodsVomits, gastric lavage and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2022-01-01
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| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/20220110?st=article_issue |
| _version_ | 1797976167342407680 |
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| author | GUO Qiaozhen ZHAO Licong ZHANG Xin ZHANG Jing SHAO Bing |
| author_facet | GUO Qiaozhen ZHAO Licong ZHANG Xin ZHANG Jing SHAO Bing |
| author_sort | GUO Qiaozhen |
| collection | DOAJ |
| description | ObjectiveScreening bio-samples from a food poisoning event based on ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF MS), and to provide strategies and references for patient treatment as well as the event response.MethodsVomits, gastric lavage and food samples were extracted by acidic acetonitrile and purified by C18 sorbent and analyzed using UPLC-QTOF MS under data independent acquisition mode. Peak identification and spectrum library retrieval were carried out by the Unifi software.ResultsHyoscyamine was detected in 5 human samples and the concentration was below 1 mg/kg. Azithromycin was also screened in one gastric lavage sample. No metabolites of hyoscyamine was found, which was consistent with the result of rat exposure experiment.ConclusionAccording to the screened result, it was inferred that this event was a family food poisoning caused by hyoscyamine. The UPLC-QTOF MS screening is of great significance in dealing with food poisoning emergencies. |
| first_indexed | 2024-04-11T04:48:09Z |
| format | Article |
| id | doaj.art-c7ba47df07f840d08be19b0d0b2ff7ee |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-04-11T04:48:09Z |
| publishDate | 2022-01-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-c7ba47df07f840d08be19b0d0b2ff7ee2022-12-27T09:24:30ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562022-01-013401495410.13590/j.cjfh.2022.01.0101004-8456(2022)01-0049-06Screening of a food poisoning caused by alkaloids by ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometryGUO Qiaozhen0ZHAO Licong1ZHANG Xin2ZHANG Jing3SHAO Bing4Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing, 100013, ChinaShijiazhuang Gaocheng District Center for Disease Control and Prevention, Heibei Shijiazhuang 052160, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing, 100013, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing, 100013, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing, 100013, ChinaObjectiveScreening bio-samples from a food poisoning event based on ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF MS), and to provide strategies and references for patient treatment as well as the event response.MethodsVomits, gastric lavage and food samples were extracted by acidic acetonitrile and purified by C18 sorbent and analyzed using UPLC-QTOF MS under data independent acquisition mode. Peak identification and spectrum library retrieval were carried out by the Unifi software.ResultsHyoscyamine was detected in 5 human samples and the concentration was below 1 mg/kg. Azithromycin was also screened in one gastric lavage sample. No metabolites of hyoscyamine was found, which was consistent with the result of rat exposure experiment.ConclusionAccording to the screened result, it was inferred that this event was a family food poisoning caused by hyoscyamine. The UPLC-QTOF MS screening is of great significance in dealing with food poisoning emergencies.http://www.zgspws.com/zgspwszz/article/abstract/20220110?st=article_issuealkaloidsfood poisoningliquid chromatography high resolution mass spectrometryhyoscyamine |
| spellingShingle | GUO Qiaozhen ZHAO Licong ZHANG Xin ZHANG Jing SHAO Bing Screening of a food poisoning caused by alkaloids by ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry Zhongguo shipin weisheng zazhi alkaloids food poisoning liquid chromatography high resolution mass spectrometry hyoscyamine |
| title | Screening of a food poisoning caused by alkaloids by ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry |
| title_full | Screening of a food poisoning caused by alkaloids by ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry |
| title_fullStr | Screening of a food poisoning caused by alkaloids by ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry |
| title_full_unstemmed | Screening of a food poisoning caused by alkaloids by ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry |
| title_short | Screening of a food poisoning caused by alkaloids by ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometry |
| title_sort | screening of a food poisoning caused by alkaloids by ultra high performance liquid chromatography quadrupole time of flight mass spectrometry |
| topic | alkaloids food poisoning liquid chromatography high resolution mass spectrometry hyoscyamine |
| url | http://www.zgspws.com/zgspwszz/article/abstract/20220110?st=article_issue |
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