Tumor-promoting effects of microRNA-421/4-aminobutyrate aminotransferase axis in hepatocellular carcinoma
Background: MicroRNA-421 (miR-421) has been implicated in hepatocellular carcinoma (HCC), but its potential mechanism in HCC remains unclear. Objectives: The study aimed to study the potential mechanism of miR-421 in HCC which is necessary. Methods: The downstream target genes of miR-421 were screen...
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| Format: | Article |
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Permanyer
2023-01-01
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| Series: | Revista de Investigación Clínica |
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| Online Access: | https://www.clinicalandtranslationalinvestigation.com/frame_esp.php?id=474 |
| _version_ | 1797640369709514752 |
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| author | Yuanguang Liu Ran Cheng Yijie Wu Chunmei Liu Yang Liu Qing Chang Jun Yin |
| author_facet | Yuanguang Liu Ran Cheng Yijie Wu Chunmei Liu Yang Liu Qing Chang Jun Yin |
| author_sort | Yuanguang Liu |
| collection | DOAJ |
| description | Background: MicroRNA-421 (miR-421) has been implicated in hepatocellular carcinoma (HCC), but its potential mechanism in HCC remains unclear. Objectives: The study aimed to study the potential mechanism of miR-421 in HCC which is necessary. Methods: The downstream target genes of miR-421 were screened in HCC tissues and cells using miDIP, Targetscan, and starBase databases. Differential analysis, survival analysis, and Pearson correlation analysis were performed between miR-421 and its downstream target genes. Quantitative reverse transcription polymerase chain reaction and western blot were used to assay RNA and protein levels of 4-aminobutyrate aminotransferase (ABAT) and epithelial-mesenchymal transition (EMT)- related proteins. Cell-based assays, including CCK-8, wound healing, transwell, flow cytometry, and metabolic measurements, were implemented to assess proliferation, migration, invasion, cell cycle, and apoptosis of HCC cells with different treatments. Dual-luciferase assay was utilized to detect the targeting relationship between miR-421 and ABAT. Results: miR-421 level was elevated in HCC tissues and cells, and low miR-421 expression hindered phenotype progression of HCC cells. ABAT was identified as a direct target of miR-421 in HCC cells, and miR-421 could inhibit ABAT expression. Rescue assay revealed that miR-421 promoted HCC cell tumorigenesis progress and affected cell metabolic remodeling through down-regulating ABAT. Conclusion: The miR-421/ABAT regulatory axis promoted HCC cell tumorigenesis progress, highlighting its potential as a therapeutic target for HCC. (
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| first_indexed | 2024-03-11T13:30:52Z |
| format | Article |
| id | doaj.art-c7bd2016002d4bc4b713ab43469946b7 |
| institution | Directory Open Access Journal |
| issn | 0034-8376 2564-8896 |
| language | English |
| last_indexed | 2024-03-11T13:30:52Z |
| publishDate | 2023-01-01 |
| publisher | Permanyer |
| record_format | Article |
| series | Revista de Investigación Clínica |
| spelling | doaj.art-c7bd2016002d4bc4b713ab43469946b72023-11-02T21:08:58ZengPermanyerRevista de Investigación Clínica0034-83762564-88962023-01-0175510.24875/RIC.23000073Tumor-promoting effects of microRNA-421/4-aminobutyrate aminotransferase axis in hepatocellular carcinomaYuanguang Liu0Ran Cheng1Yijie Wu2Chunmei Liu3Yang Liu4Qing Chang5Jun Yin6Department of Hepatobiliary Surgery, Tangshan Gongren Hospital, Tangshan, ChinaDepartment of Hepatobiliary Surgery, Tangshan Gongren Hospital, Tangshan, ChinaDepartment of Hepatobiliary Surgery, Tangshan Gongren Hospital, Tangshan, ChinaDepartment of Hepatobiliary Surgery, Tangshan Gongren Hospital, Tangshan, ChinaDepartment of Hepatobiliary Surgery, Tangshan Gongren Hospital, Tangshan, ChinaDepartment of Head and Neck Surgery, Tangshan Gongren Hospital, Tangshan, ChinaDepartment of Hepatobiliary Surgery, Tangshan Gongren Hospital, Tangshan, ChinaBackground: MicroRNA-421 (miR-421) has been implicated in hepatocellular carcinoma (HCC), but its potential mechanism in HCC remains unclear. Objectives: The study aimed to study the potential mechanism of miR-421 in HCC which is necessary. Methods: The downstream target genes of miR-421 were screened in HCC tissues and cells using miDIP, Targetscan, and starBase databases. Differential analysis, survival analysis, and Pearson correlation analysis were performed between miR-421 and its downstream target genes. Quantitative reverse transcription polymerase chain reaction and western blot were used to assay RNA and protein levels of 4-aminobutyrate aminotransferase (ABAT) and epithelial-mesenchymal transition (EMT)- related proteins. Cell-based assays, including CCK-8, wound healing, transwell, flow cytometry, and metabolic measurements, were implemented to assess proliferation, migration, invasion, cell cycle, and apoptosis of HCC cells with different treatments. Dual-luciferase assay was utilized to detect the targeting relationship between miR-421 and ABAT. Results: miR-421 level was elevated in HCC tissues and cells, and low miR-421 expression hindered phenotype progression of HCC cells. ABAT was identified as a direct target of miR-421 in HCC cells, and miR-421 could inhibit ABAT expression. Rescue assay revealed that miR-421 promoted HCC cell tumorigenesis progress and affected cell metabolic remodeling through down-regulating ABAT. Conclusion: The miR-421/ABAT regulatory axis promoted HCC cell tumorigenesis progress, highlighting its potential as a therapeutic target for HCC. ( https://www.clinicalandtranslationalinvestigation.com/frame_esp.php?id=474Hepatocellular carcinoma. MicroRNA-421. 4-aminobutyrate aminotransferase. Cell phenotype. Epithelial-mesenchymal transition. |
| spellingShingle | Yuanguang Liu Ran Cheng Yijie Wu Chunmei Liu Yang Liu Qing Chang Jun Yin Tumor-promoting effects of microRNA-421/4-aminobutyrate aminotransferase axis in hepatocellular carcinoma Revista de Investigación Clínica Hepatocellular carcinoma. MicroRNA-421. 4-aminobutyrate aminotransferase. Cell phenotype. Epithelial-mesenchymal transition. |
| title | Tumor-promoting effects of microRNA-421/4-aminobutyrate aminotransferase axis in hepatocellular carcinoma |
| title_full | Tumor-promoting effects of microRNA-421/4-aminobutyrate aminotransferase axis in hepatocellular carcinoma |
| title_fullStr | Tumor-promoting effects of microRNA-421/4-aminobutyrate aminotransferase axis in hepatocellular carcinoma |
| title_full_unstemmed | Tumor-promoting effects of microRNA-421/4-aminobutyrate aminotransferase axis in hepatocellular carcinoma |
| title_short | Tumor-promoting effects of microRNA-421/4-aminobutyrate aminotransferase axis in hepatocellular carcinoma |
| title_sort | tumor promoting effects of microrna 421 4 aminobutyrate aminotransferase axis in hepatocellular carcinoma |
| topic | Hepatocellular carcinoma. MicroRNA-421. 4-aminobutyrate aminotransferase. Cell phenotype. Epithelial-mesenchymal transition. |
| url | https://www.clinicalandtranslationalinvestigation.com/frame_esp.php?id=474 |
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