Bacillus halotolerans KKD1 induces physiological, metabolic and molecular reprogramming in wheat under saline condition
Salt stress decreases plant growth and is a major threat to crop yields worldwide. The present study aimed to alleviate salt stress in plants by inoculation with halophilic plant growth-promoting rhizobacteria (PGPR) isolated from an extreme environment in the Qinghai–Tibetan Plateau. Wheat plants i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.978066/full |
| _version_ | 1811217232796057600 |
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| author | Xiaohui Wu Xiaohui Wu Yaning Fan Ruoyi Wang Qian Zhao Qurban Ali Huijun Wu Qin Gu Rainer Borriss Rainer Borriss Yongli Xie Xuewen Gao |
| author_facet | Xiaohui Wu Xiaohui Wu Yaning Fan Ruoyi Wang Qian Zhao Qurban Ali Huijun Wu Qin Gu Rainer Borriss Rainer Borriss Yongli Xie Xuewen Gao |
| author_sort | Xiaohui Wu |
| collection | DOAJ |
| description | Salt stress decreases plant growth and is a major threat to crop yields worldwide. The present study aimed to alleviate salt stress in plants by inoculation with halophilic plant growth-promoting rhizobacteria (PGPR) isolated from an extreme environment in the Qinghai–Tibetan Plateau. Wheat plants inoculated with Bacillus halotolerans KKD1 showed increased seedling morphological parameters and physiological indexes. The expression of wheat genes directly involved in plant growth was upregulated in the presence of KKD1, as shown by real-time quantitative PCR (RT-qPCR) analysis. The metabolism of phytohormones, such as 6-benzylaminopurine and gibberellic acid were also enhanced. Mining of the KKD1 genome corroborated its potential plant growth promotion (PGP) and biocontrol properties. Moreover, KKD1 was able to support plant growth under salt stress by inducing a stress response in wheat by modulating phytohormone levels, regulating lipid peroxidation, accumulating betaine, and excluding Na+. In addition, KKD1 positively affected the soil nitrogen content, soil phosphorus content and soil pH. Our findings indicated that KKD1 is a promising candidate for encouraging wheat plant growth under saline conditions. |
| first_indexed | 2024-04-12T06:50:57Z |
| format | Article |
| id | doaj.art-c7bd435d85fc43d197b3b88546f9dd57 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T06:50:57Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c7bd435d85fc43d197b3b88546f9dd572022-12-22T03:43:21ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-08-011310.3389/fpls.2022.978066978066Bacillus halotolerans KKD1 induces physiological, metabolic and molecular reprogramming in wheat under saline conditionXiaohui Wu0Xiaohui Wu1Yaning Fan2Ruoyi Wang3Qian Zhao4Qurban Ali5Huijun Wu6Qin Gu7Rainer Borriss8Rainer Borriss9Yongli Xie10Xuewen Gao11Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, ChinaState Key Laboratory of Plateau Ecology and Agriculture, Department of Grassland Science, College of Agricultural and Husbandry, Qinghai University, Xining, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, ChinaInstitut für Biologie, Humboldt Universität, Berlin, GermanyNord Reet UG, Greifswald, GermanyState Key Laboratory of Plateau Ecology and Agriculture, Department of Grassland Science, College of Agricultural and Husbandry, Qinghai University, Xining, ChinaKey Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, ChinaSalt stress decreases plant growth and is a major threat to crop yields worldwide. The present study aimed to alleviate salt stress in plants by inoculation with halophilic plant growth-promoting rhizobacteria (PGPR) isolated from an extreme environment in the Qinghai–Tibetan Plateau. Wheat plants inoculated with Bacillus halotolerans KKD1 showed increased seedling morphological parameters and physiological indexes. The expression of wheat genes directly involved in plant growth was upregulated in the presence of KKD1, as shown by real-time quantitative PCR (RT-qPCR) analysis. The metabolism of phytohormones, such as 6-benzylaminopurine and gibberellic acid were also enhanced. Mining of the KKD1 genome corroborated its potential plant growth promotion (PGP) and biocontrol properties. Moreover, KKD1 was able to support plant growth under salt stress by inducing a stress response in wheat by modulating phytohormone levels, regulating lipid peroxidation, accumulating betaine, and excluding Na+. In addition, KKD1 positively affected the soil nitrogen content, soil phosphorus content and soil pH. Our findings indicated that KKD1 is a promising candidate for encouraging wheat plant growth under saline conditions.https://www.frontiersin.org/articles/10.3389/fpls.2022.978066/fullBacillus halotoleransPGPRgenome analysissalt stressplant-stress responseplant physiological and metabolism |
| spellingShingle | Xiaohui Wu Xiaohui Wu Yaning Fan Ruoyi Wang Qian Zhao Qurban Ali Huijun Wu Qin Gu Rainer Borriss Rainer Borriss Yongli Xie Xuewen Gao Bacillus halotolerans KKD1 induces physiological, metabolic and molecular reprogramming in wheat under saline condition Frontiers in Plant Science Bacillus halotolerans PGPR genome analysis salt stress plant-stress response plant physiological and metabolism |
| title | Bacillus halotolerans KKD1 induces physiological, metabolic and molecular reprogramming in wheat under saline condition |
| title_full | Bacillus halotolerans KKD1 induces physiological, metabolic and molecular reprogramming in wheat under saline condition |
| title_fullStr | Bacillus halotolerans KKD1 induces physiological, metabolic and molecular reprogramming in wheat under saline condition |
| title_full_unstemmed | Bacillus halotolerans KKD1 induces physiological, metabolic and molecular reprogramming in wheat under saline condition |
| title_short | Bacillus halotolerans KKD1 induces physiological, metabolic and molecular reprogramming in wheat under saline condition |
| title_sort | bacillus halotolerans kkd1 induces physiological metabolic and molecular reprogramming in wheat under saline condition |
| topic | Bacillus halotolerans PGPR genome analysis salt stress plant-stress response plant physiological and metabolism |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.978066/full |
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