Protocol for intranasal chemoprevention delivery in a urethane mouse lung cancer model
Summary: Mouse iloprost lung cancer chemoprevention studies typically use oral delivery. Here, we present a protocol for intranasal iloprost delivery within a urethane lung adenocarcinoma mouse model. We detail steps for intraperitoneal urethane injection in mice, followed by nine-week monitoring, i...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672200630X |
| _version_ | 1797980179459473408 |
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| author | Lori Dwyer-Nield Robert L. Keith Meredith A. Tennis |
| author_facet | Lori Dwyer-Nield Robert L. Keith Meredith A. Tennis |
| author_sort | Lori Dwyer-Nield |
| collection | DOAJ |
| description | Summary: Mouse iloprost lung cancer chemoprevention studies typically use oral delivery. Here, we present a protocol for intranasal iloprost delivery within a urethane lung adenocarcinoma mouse model. We detail steps for intraperitoneal urethane injection in mice, followed by nine-week monitoring, intranasal iloprost treatment, and lungs harvesting for analysis. This iloprost delivery approach parallels an ongoing phase II clinical trial of inhaled iloprost for lung cancer chemoprevention. This protocol diversifies options for chemoprevention studies and offers a relevant and translatable model.For complete details on the use and execution of this protocol, please refer to Sompel et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T05:50:18Z |
| format | Article |
| id | doaj.art-c7c10e77f2c642ec88b0ad1040249963 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T05:50:18Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-c7c10e77f2c642ec88b0ad10402499632022-12-22T04:42:05ZengElsevierSTAR Protocols2666-16672022-12-0134101750Protocol for intranasal chemoprevention delivery in a urethane mouse lung cancer modelLori Dwyer-Nield0Robert L. Keith1Meredith A. Tennis2University of Colorado School of Pharmacy, Aurora, CO 80045, USARocky Mountain Regional VA Medical Center, Aurora, CO 80045, USA; University of Colorado School of Medicine, Aurora, CO 80045, USAUniversity of Colorado School of Medicine, Aurora, CO 80045, USA; Corresponding authorSummary: Mouse iloprost lung cancer chemoprevention studies typically use oral delivery. Here, we present a protocol for intranasal iloprost delivery within a urethane lung adenocarcinoma mouse model. We detail steps for intraperitoneal urethane injection in mice, followed by nine-week monitoring, intranasal iloprost treatment, and lungs harvesting for analysis. This iloprost delivery approach parallels an ongoing phase II clinical trial of inhaled iloprost for lung cancer chemoprevention. This protocol diversifies options for chemoprevention studies and offers a relevant and translatable model.For complete details on the use and execution of this protocol, please refer to Sompel et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672200630XCancerModel Organisms |
| spellingShingle | Lori Dwyer-Nield Robert L. Keith Meredith A. Tennis Protocol for intranasal chemoprevention delivery in a urethane mouse lung cancer model STAR Protocols Cancer Model Organisms |
| title | Protocol for intranasal chemoprevention delivery in a urethane mouse lung cancer model |
| title_full | Protocol for intranasal chemoprevention delivery in a urethane mouse lung cancer model |
| title_fullStr | Protocol for intranasal chemoprevention delivery in a urethane mouse lung cancer model |
| title_full_unstemmed | Protocol for intranasal chemoprevention delivery in a urethane mouse lung cancer model |
| title_short | Protocol for intranasal chemoprevention delivery in a urethane mouse lung cancer model |
| title_sort | protocol for intranasal chemoprevention delivery in a urethane mouse lung cancer model |
| topic | Cancer Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S266616672200630X |
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