A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the numbe...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2004-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012 |
| _version_ | 1819103840756039680 |
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| author | Maríndia Deprá Lenira Maria Nunes Sepel Élgion Lucio da Silva Loreto |
| author_facet | Maríndia Deprá Lenira Maria Nunes Sepel Élgion Lucio da Silva Loreto |
| author_sort | Maríndia Deprá |
| collection | DOAJ |
| description | We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation. |
| first_indexed | 2024-12-22T01:56:51Z |
| format | Article |
| id | doaj.art-c7c2a5b6bf124613b4b9d68b4d24773d |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-22T01:56:51Z |
| publishDate | 2004-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-c7c2a5b6bf124613b4b9d68b4d24773d2022-12-21T18:42:46ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852004-01-01271707310.1590/S1415-47572004000100012A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markersMaríndia DepráLenira Maria Nunes SepelÉlgion Lucio da Silva LoretoWe describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012transgenic DrosophilapiggyBacGFPtransposable elements |
| spellingShingle | Maríndia Deprá Lenira Maria Nunes Sepel Élgion Lucio da Silva Loreto A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers Genetics and Molecular Biology transgenic Drosophila piggyBac GFP transposable elements |
| title | A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers |
| title_full | A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers |
| title_fullStr | A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers |
| title_full_unstemmed | A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers |
| title_short | A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers |
| title_sort | low cost apparatus for transforming drosophila and detecting green fluorescent protein gfp genetic markers |
| topic | transgenic Drosophila piggyBac GFP transposable elements |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012 |
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