Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia
Background: Heat shock protein-70 (Hsp-70) exhibits cytoprotective effects against oxidative stress-induced airway injury. This study aimed to examine Hsp-70 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) from tracheal aspirates (TA) in very low-birth weight (VLBW) preterm infants to predict the developme...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Pediatrics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fped.2021.616452/full |
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| author | Chien-Chou Hsiao Chien-Chou Hsiao Chien-Chou Hsiao Cheng-Han Lee Rei-Cheng Yang Rei-Cheng Yang Jia-Yuh Chen Tzu-Cheng Su Yu-Jun Chang Ching-Yuang Lin Yi-Giien Tsai Yi-Giien Tsai Yi-Giien Tsai |
| author_facet | Chien-Chou Hsiao Chien-Chou Hsiao Chien-Chou Hsiao Cheng-Han Lee Rei-Cheng Yang Rei-Cheng Yang Jia-Yuh Chen Tzu-Cheng Su Yu-Jun Chang Ching-Yuang Lin Yi-Giien Tsai Yi-Giien Tsai Yi-Giien Tsai |
| author_sort | Chien-Chou Hsiao |
| collection | DOAJ |
| description | Background: Heat shock protein-70 (Hsp-70) exhibits cytoprotective effects against oxidative stress-induced airway injury. This study aimed to examine Hsp-70 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) from tracheal aspirates (TA) in very low-birth weight (VLBW) preterm infants to predict the development of bronchopulmonary dysplasia (BPD).Methods: This birth cohort study enrolled 109 VLBW preterm infants, including 32 infants who developed BPD. Hsp-70 and 8-OHdG concentrations from TA were measured by immunoassay. The apoptosis of TA epithelial cells obtained on Day 28 after birth was measured using annexin-V staining assay.Results: Hsp-70 and 8-OHdG levels in TA fluid were persistently increased from Day 1 to Day 28 of life in the BPD group. Multiple linear regression analysis demonstrated that BPD was significantly associated with gestational age, respiratory distress syndrome, and TA Hsp-70 and 8-OHdG levels on post-natal Day 28. The TA Hsp-70 level positively correlated with TA 8-OHdG level on the Day 1 (r = 0.47) and Day 28 of life (r = 0.68). Incubation of recombinant Hsp-70 with primary epithelial cells derived from TA of patients decreased hydrogen peroxide-induced epithelial cell death.Conclusions: Hsp-70 levels are associated with a state of oxidative injury in the development of BPD. |
| first_indexed | 2024-12-22T10:30:53Z |
| format | Article |
| id | doaj.art-c7c4165ecd73454c8240d2fef797e117 |
| institution | Directory Open Access Journal |
| issn | 2296-2360 |
| language | English |
| last_indexed | 2024-12-22T10:30:53Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pediatrics |
| spelling | doaj.art-c7c4165ecd73454c8240d2fef797e1172022-12-21T18:29:20ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602021-05-01910.3389/fped.2021.616452616452Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary DysplasiaChien-Chou Hsiao0Chien-Chou Hsiao1Chien-Chou Hsiao2Cheng-Han Lee3Rei-Cheng Yang4Rei-Cheng Yang5Jia-Yuh Chen6Tzu-Cheng Su7Yu-Jun Chang8Ching-Yuang Lin9Yi-Giien Tsai10Yi-Giien Tsai11Yi-Giien Tsai12Department of Pediatrics, Changhua Christian Children's Hospital, Changhua, TaiwanSchool of Medicine, Kaohsiung Medical University, Kaohsiung, TaiwanSchool of Medicine, Chung Shan Medical University, Taichung, TaiwanDepartment of Pediatrics, Changhua Christian Children's Hospital, Changhua, TaiwanSchool of Medicine, Kaohsiung Medical University, Kaohsiung, TaiwanDepartment of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung, TaiwanDepartment of Pediatrics, Changhua Christian Children's Hospital, Changhua, TaiwanDepartment of Pathology, Changhua Christian Hospital, Changhua, TaiwanEpidemiology and Biostatistics and Big Data Center, Changhua Christian Hospital, Changhua, TaiwanDivision of Pediatric Nephrology, Children's Hospital, China Medical University, Taichung, TaiwanDepartment of Pediatrics, Changhua Christian Children's Hospital, Changhua, TaiwanSchool of Medicine, Kaohsiung Medical University, Kaohsiung, TaiwanSchool of Medicine, Chung Shan Medical University, Taichung, TaiwanBackground: Heat shock protein-70 (Hsp-70) exhibits cytoprotective effects against oxidative stress-induced airway injury. This study aimed to examine Hsp-70 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) from tracheal aspirates (TA) in very low-birth weight (VLBW) preterm infants to predict the development of bronchopulmonary dysplasia (BPD).Methods: This birth cohort study enrolled 109 VLBW preterm infants, including 32 infants who developed BPD. Hsp-70 and 8-OHdG concentrations from TA were measured by immunoassay. The apoptosis of TA epithelial cells obtained on Day 28 after birth was measured using annexin-V staining assay.Results: Hsp-70 and 8-OHdG levels in TA fluid were persistently increased from Day 1 to Day 28 of life in the BPD group. Multiple linear regression analysis demonstrated that BPD was significantly associated with gestational age, respiratory distress syndrome, and TA Hsp-70 and 8-OHdG levels on post-natal Day 28. The TA Hsp-70 level positively correlated with TA 8-OHdG level on the Day 1 (r = 0.47) and Day 28 of life (r = 0.68). Incubation of recombinant Hsp-70 with primary epithelial cells derived from TA of patients decreased hydrogen peroxide-induced epithelial cell death.Conclusions: Hsp-70 levels are associated with a state of oxidative injury in the development of BPD.https://www.frontiersin.org/articles/10.3389/fped.2021.616452/fullheat shock protein-70bronchopulmonary dysplasiapreterm infantsapoptosisoxidative stress |
| spellingShingle | Chien-Chou Hsiao Chien-Chou Hsiao Chien-Chou Hsiao Cheng-Han Lee Rei-Cheng Yang Rei-Cheng Yang Jia-Yuh Chen Tzu-Cheng Su Yu-Jun Chang Ching-Yuang Lin Yi-Giien Tsai Yi-Giien Tsai Yi-Giien Tsai Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia Frontiers in Pediatrics heat shock protein-70 bronchopulmonary dysplasia preterm infants apoptosis oxidative stress |
| title | Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia |
| title_full | Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia |
| title_fullStr | Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia |
| title_full_unstemmed | Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia |
| title_short | Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia |
| title_sort | heat shock protein 70 levels are associated with a state of oxidative damage in the development of bronchopulmonary dysplasia |
| topic | heat shock protein-70 bronchopulmonary dysplasia preterm infants apoptosis oxidative stress |
| url | https://www.frontiersin.org/articles/10.3389/fped.2021.616452/full |
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