Enhancement of Vancomycin Potential against Pathogenic Bacterial Strains via Gold Nano-Formulations: A Nano-Antibiotic Approach
The remarkable rise of antibiotic resistance among pathogenic bacteria poses a significant threat to human health. Nanoparticles (NPs) have recently emerged as novel strategies for conquering fatal bacterial diseases. Furthermore, antibiotic-functionalized metallic NPs represent a viable nano-platfo...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/1996-1944/15/3/1108 |
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| author | Turki Al Hagbani Hemant Yadav Afrasim Moin Amr Selim Abu Lila Khalid Mehmood Farhan Alshammari Salman Khan El-Sayed Khafagy Talib Hussain Syed Mohd Danish Rizvi Marwa H. Abdallah |
| author_facet | Turki Al Hagbani Hemant Yadav Afrasim Moin Amr Selim Abu Lila Khalid Mehmood Farhan Alshammari Salman Khan El-Sayed Khafagy Talib Hussain Syed Mohd Danish Rizvi Marwa H. Abdallah |
| author_sort | Turki Al Hagbani |
| collection | DOAJ |
| description | The remarkable rise of antibiotic resistance among pathogenic bacteria poses a significant threat to human health. Nanoparticles (NPs) have recently emerged as novel strategies for conquering fatal bacterial diseases. Furthermore, antibiotic-functionalized metallic NPs represent a viable nano-platform for combating bacterial resistance. In this study, we present the use of vancomycin-functionalized gold nanoparticles (V-GNPs) to battle pathogenic bacterial strains. A facile one-pot method was adopted to synthesize vancomycin-loaded GNPs in which the reducing properties of vancomycin were exploited to produce V-GNPs from gold ions. UV–Visible spectroscopy verified the production of V-GNPs via the existence of a surface plasmon resonance peak at 524 nm, whereas transmission electron microscopy depicted a size of ~24 nm. Further, dynamic light scattering (DLS) estimated the hydrodynamic diameter as 77 nm. The stability of V-GNPs was investigated using zeta-potential measurements, and the zeta potential of V-GNPs was found to be −18 mV. Fourier transform infrared spectroscopy confirmed the efficient loading of vancomycin onto GNP surfaces; however, the loading efficiency of vancomycin onto V-GNPs was 86.2%. Finally, in vitro antibacterial studies revealed that V-GNPs were much more effective, even at lower concentrations, than pure vancomycin. The observed antibacterial activities of V-GNPs were 1.4-, 1.6-, 1.8-, and 1.6-fold higher against Gram-negative <i>Escherichia coli</i>, <i>Klebsiella oxytoca</i>, and <i>Pseudomonas aeruginosa</i> and Gram-positive <i>Staphylococcus aureus</i>, respectively, compared to pure vancomycin. Collectively, V-GNPs represented a more viable alternative to pure vancomycin, even at a lower antibiotic dose, in conquering pathogenic bacteria. |
| first_indexed | 2024-03-09T23:35:27Z |
| format | Article |
| id | doaj.art-c7c6989d8ff941048b7b43b2c7c99491 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T23:35:27Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-c7c6989d8ff941048b7b43b2c7c994912023-11-23T17:02:26ZengMDPI AGMaterials1996-19442022-01-01153110810.3390/ma15031108Enhancement of Vancomycin Potential against Pathogenic Bacterial Strains via Gold Nano-Formulations: A Nano-Antibiotic ApproachTurki Al Hagbani0Hemant Yadav1Afrasim Moin2Amr Selim Abu Lila3Khalid Mehmood4Farhan Alshammari5Salman Khan6El-Sayed Khafagy7Talib Hussain8Syed Mohd Danish Rizvi9Marwa H. Abdallah10Department of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmaceutics, RAK College of Pharmaceutical Sciences, RAK Medical & Health Sciences University, Ras Al Khaimah 11172, United Arab EmiratesDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmacy, Abbottabad University of Science and Technology, Havelian 22010, PakistanDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaNanomedicine and Nanotechnology Lab, Department of Biosciences, Integral University, Lucknow 226026, IndiaDepartment of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-kharj 11942, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaThe remarkable rise of antibiotic resistance among pathogenic bacteria poses a significant threat to human health. Nanoparticles (NPs) have recently emerged as novel strategies for conquering fatal bacterial diseases. Furthermore, antibiotic-functionalized metallic NPs represent a viable nano-platform for combating bacterial resistance. In this study, we present the use of vancomycin-functionalized gold nanoparticles (V-GNPs) to battle pathogenic bacterial strains. A facile one-pot method was adopted to synthesize vancomycin-loaded GNPs in which the reducing properties of vancomycin were exploited to produce V-GNPs from gold ions. UV–Visible spectroscopy verified the production of V-GNPs via the existence of a surface plasmon resonance peak at 524 nm, whereas transmission electron microscopy depicted a size of ~24 nm. Further, dynamic light scattering (DLS) estimated the hydrodynamic diameter as 77 nm. The stability of V-GNPs was investigated using zeta-potential measurements, and the zeta potential of V-GNPs was found to be −18 mV. Fourier transform infrared spectroscopy confirmed the efficient loading of vancomycin onto GNP surfaces; however, the loading efficiency of vancomycin onto V-GNPs was 86.2%. Finally, in vitro antibacterial studies revealed that V-GNPs were much more effective, even at lower concentrations, than pure vancomycin. The observed antibacterial activities of V-GNPs were 1.4-, 1.6-, 1.8-, and 1.6-fold higher against Gram-negative <i>Escherichia coli</i>, <i>Klebsiella oxytoca</i>, and <i>Pseudomonas aeruginosa</i> and Gram-positive <i>Staphylococcus aureus</i>, respectively, compared to pure vancomycin. Collectively, V-GNPs represented a more viable alternative to pure vancomycin, even at a lower antibiotic dose, in conquering pathogenic bacteria.https://www.mdpi.com/1996-1944/15/3/1108gold nanoparticlesantibiotic resistancevancomycinmetallic nanoparticlestransmission electron microscopy |
| spellingShingle | Turki Al Hagbani Hemant Yadav Afrasim Moin Amr Selim Abu Lila Khalid Mehmood Farhan Alshammari Salman Khan El-Sayed Khafagy Talib Hussain Syed Mohd Danish Rizvi Marwa H. Abdallah Enhancement of Vancomycin Potential against Pathogenic Bacterial Strains via Gold Nano-Formulations: A Nano-Antibiotic Approach Materials gold nanoparticles antibiotic resistance vancomycin metallic nanoparticles transmission electron microscopy |
| title | Enhancement of Vancomycin Potential against Pathogenic Bacterial Strains via Gold Nano-Formulations: A Nano-Antibiotic Approach |
| title_full | Enhancement of Vancomycin Potential against Pathogenic Bacterial Strains via Gold Nano-Formulations: A Nano-Antibiotic Approach |
| title_fullStr | Enhancement of Vancomycin Potential against Pathogenic Bacterial Strains via Gold Nano-Formulations: A Nano-Antibiotic Approach |
| title_full_unstemmed | Enhancement of Vancomycin Potential against Pathogenic Bacterial Strains via Gold Nano-Formulations: A Nano-Antibiotic Approach |
| title_short | Enhancement of Vancomycin Potential against Pathogenic Bacterial Strains via Gold Nano-Formulations: A Nano-Antibiotic Approach |
| title_sort | enhancement of vancomycin potential against pathogenic bacterial strains via gold nano formulations a nano antibiotic approach |
| topic | gold nanoparticles antibiotic resistance vancomycin metallic nanoparticles transmission electron microscopy |
| url | https://www.mdpi.com/1996-1944/15/3/1108 |
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