Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community
A double color terminal restriction fragment length polymorphism analysis was applied for the diversity assessment of a complex bacterial community in a deciduous forest soil. Application of the second label was used to project a specific taxonomic group of bacteria, actinomycetes in our case, to th...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2009-09-01
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| Series: | Plant, Soil and Environment |
| Subjects: | |
| Online Access: | https://pse.agriculturejournals.cz/artkey/pse-200909-0005_modification-of-the-terminal-restriction-fragment-length-polymorphism-analysisfor-assessment-of-a-specific-taxo.php |
| _version_ | 1828007504450158592 |
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| author | J. Kopecký G. Novotná M. Ságová-Marečková |
| author_facet | J. Kopecký G. Novotná M. Ságová-Marečková |
| author_sort | J. Kopecký |
| collection | DOAJ |
| description | A double color terminal restriction fragment length polymorphism analysis was applied for the diversity assessment of a complex bacterial community in a deciduous forest soil. Application of the second label was used to project a specific taxonomic group of bacteria, actinomycetes in our case, to the universal fingerprint. The diversity of actinomycetes was evaluated directly as a percentage of the whole bacterial community by comparing the corresponding terminal fragments of the same length. |
| first_indexed | 2024-04-10T08:12:25Z |
| format | Article |
| id | doaj.art-c7c6d5a462ba4d89ac8d86987049c7f7 |
| institution | Directory Open Access Journal |
| issn | 1214-1178 1805-9368 |
| language | English |
| last_indexed | 2024-04-10T08:12:25Z |
| publishDate | 2009-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant, Soil and Environment |
| spelling | doaj.art-c7c6d5a462ba4d89ac8d86987049c7f72023-02-23T03:45:31ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682009-09-0155939740310.17221/1012-PSEpse-200909-0005Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial communityJ. Kopecký0G. Novotná1M. Ságová-Marečková2Crop Research Institute, Prague, Czech RepublicCrop Research Institute, Prague, Czech RepublicCrop Research Institute, Prague, Czech RepublicA double color terminal restriction fragment length polymorphism analysis was applied for the diversity assessment of a complex bacterial community in a deciduous forest soil. Application of the second label was used to project a specific taxonomic group of bacteria, actinomycetes in our case, to the universal fingerprint. The diversity of actinomycetes was evaluated directly as a percentage of the whole bacterial community by comparing the corresponding terminal fragments of the same length.https://pse.agriculturejournals.cz/artkey/pse-200909-0005_modification-of-the-terminal-restriction-fragment-length-polymorphism-analysisfor-assessment-of-a-specific-taxo.phpbacterial communityactinobacteriaterminal restriction fragment length polymorphism |
| spellingShingle | J. Kopecký G. Novotná M. Ságová-Marečková Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community Plant, Soil and Environment bacterial community actinobacteria terminal restriction fragment length polymorphism |
| title | Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community |
| title_full | Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community |
| title_fullStr | Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community |
| title_full_unstemmed | Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community |
| title_short | Modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community |
| title_sort | modification of the terminal restriction fragment length polymorphism analysisfor assessment of a specific taxonomic group within a soil microbial community |
| topic | bacterial community actinobacteria terminal restriction fragment length polymorphism |
| url | https://pse.agriculturejournals.cz/artkey/pse-200909-0005_modification-of-the-terminal-restriction-fragment-length-polymorphism-analysisfor-assessment-of-a-specific-taxo.php |
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