Regulation of Embden–Meyerhof–Parnas (EMP) Pathway and Tricarboxylic Acid (TCA) Cycle Concerning Aberrant Chilling Injury Behavior in Postharvest Papaya (<i>Carica papaya</i> L.)
Postharvest abnormal chilling injury (CI) behavior in papaya (<i>Carica papaya</i> L.) fruit is a rare phenomenon that may be associated with respiratory metabolism. This study thus aimed to investigate the impacts of storage temperatures (1 and 6 °C) on the respiratory metabolism of pos...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13898 |
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| author | Lijin Huang Shoukui Tao Yi Zhu Yonggui Pan Zhengke Zhang Zhiqian Yu Yezhen Chen |
| author_facet | Lijin Huang Shoukui Tao Yi Zhu Yonggui Pan Zhengke Zhang Zhiqian Yu Yezhen Chen |
| author_sort | Lijin Huang |
| collection | DOAJ |
| description | Postharvest abnormal chilling injury (CI) behavior in papaya (<i>Carica papaya</i> L.) fruit is a rare phenomenon that may be associated with respiratory metabolism. This study thus aimed to investigate the impacts of storage temperatures (1 and 6 °C) on the respiratory metabolism of postharvest papaya and its impact on CI development. Results demonstrated that 1 °C storage reduced the activities of hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), citrate synthase (CS), and α-ketoglutarate dehydrogenase (α-KGDH) and regulated the expression of corresponding enzymes in the Embden–Meyerhof–Parnas (EMP) pathway and tricarboxylic acid (TCA) cycle compared with 6 °C storage, resulting in a lower respiration rate of the EMP-TCA pathway and mitigating the development of CI. Meanwhile, lower contents of nicotinamide adenine dinucleotide (hydrogen) (NAD(H)) were observed in papaya fruit stored at 1 °C. Notably, papaya fruit stored at 1 °C maintained higher activity and transcriptional levels of SDH and IDH during the whole storage period. These findings suggest that 1 °C storage reduced the respiration rate of the EMP-TCA pathway by reducing the expression level and activity of related enzymes, which is conducive to the reduction of respiration substrate consumption and finally alleviating the occurrence of CI. |
| first_indexed | 2024-03-10T22:40:37Z |
| format | Article |
| id | doaj.art-c7c837bcb4634f039534ed1282efc141 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:40:37Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c7c837bcb4634f039534ed1282efc1412023-11-19T11:04:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181389810.3390/ijms241813898Regulation of Embden–Meyerhof–Parnas (EMP) Pathway and Tricarboxylic Acid (TCA) Cycle Concerning Aberrant Chilling Injury Behavior in Postharvest Papaya (<i>Carica papaya</i> L.)Lijin Huang0Shoukui Tao1Yi Zhu2Yonggui Pan3Zhengke Zhang4Zhiqian Yu5Yezhen Chen6School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, Hainan University, Haikou 570228, ChinaPostharvest abnormal chilling injury (CI) behavior in papaya (<i>Carica papaya</i> L.) fruit is a rare phenomenon that may be associated with respiratory metabolism. This study thus aimed to investigate the impacts of storage temperatures (1 and 6 °C) on the respiratory metabolism of postharvest papaya and its impact on CI development. Results demonstrated that 1 °C storage reduced the activities of hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), citrate synthase (CS), and α-ketoglutarate dehydrogenase (α-KGDH) and regulated the expression of corresponding enzymes in the Embden–Meyerhof–Parnas (EMP) pathway and tricarboxylic acid (TCA) cycle compared with 6 °C storage, resulting in a lower respiration rate of the EMP-TCA pathway and mitigating the development of CI. Meanwhile, lower contents of nicotinamide adenine dinucleotide (hydrogen) (NAD(H)) were observed in papaya fruit stored at 1 °C. Notably, papaya fruit stored at 1 °C maintained higher activity and transcriptional levels of SDH and IDH during the whole storage period. These findings suggest that 1 °C storage reduced the respiration rate of the EMP-TCA pathway by reducing the expression level and activity of related enzymes, which is conducive to the reduction of respiration substrate consumption and finally alleviating the occurrence of CI.https://www.mdpi.com/1422-0067/24/18/13898papayachilling injuryrespiration pathwayEMP pathwayTCA cycle |
| spellingShingle | Lijin Huang Shoukui Tao Yi Zhu Yonggui Pan Zhengke Zhang Zhiqian Yu Yezhen Chen Regulation of Embden–Meyerhof–Parnas (EMP) Pathway and Tricarboxylic Acid (TCA) Cycle Concerning Aberrant Chilling Injury Behavior in Postharvest Papaya (<i>Carica papaya</i> L.) International Journal of Molecular Sciences papaya chilling injury respiration pathway EMP pathway TCA cycle |
| title | Regulation of Embden–Meyerhof–Parnas (EMP) Pathway and Tricarboxylic Acid (TCA) Cycle Concerning Aberrant Chilling Injury Behavior in Postharvest Papaya (<i>Carica papaya</i> L.) |
| title_full | Regulation of Embden–Meyerhof–Parnas (EMP) Pathway and Tricarboxylic Acid (TCA) Cycle Concerning Aberrant Chilling Injury Behavior in Postharvest Papaya (<i>Carica papaya</i> L.) |
| title_fullStr | Regulation of Embden–Meyerhof–Parnas (EMP) Pathway and Tricarboxylic Acid (TCA) Cycle Concerning Aberrant Chilling Injury Behavior in Postharvest Papaya (<i>Carica papaya</i> L.) |
| title_full_unstemmed | Regulation of Embden–Meyerhof–Parnas (EMP) Pathway and Tricarboxylic Acid (TCA) Cycle Concerning Aberrant Chilling Injury Behavior in Postharvest Papaya (<i>Carica papaya</i> L.) |
| title_short | Regulation of Embden–Meyerhof–Parnas (EMP) Pathway and Tricarboxylic Acid (TCA) Cycle Concerning Aberrant Chilling Injury Behavior in Postharvest Papaya (<i>Carica papaya</i> L.) |
| title_sort | regulation of embden meyerhof parnas emp pathway and tricarboxylic acid tca cycle concerning aberrant chilling injury behavior in postharvest papaya i carica papaya i l |
| topic | papaya chilling injury respiration pathway EMP pathway TCA cycle |
| url | https://www.mdpi.com/1422-0067/24/18/13898 |
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