Beneficial effects of a novel shark-skin collagen dressing for the promotion of seawater immersion wound healing
Abstract Background Wounded personnel who work at sea often encounter a plethora of difficulties. The most important of these difficulties is seawater immersion. Common medical dressings have little effect when the affected area is immersed in seawater, and only rarely dressings have been reported f...
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| Format: | Article |
| Language: | English |
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BMC
2017-10-01
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| Series: | Military Medical Research |
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| Online Access: | http://link.springer.com/article/10.1186/s40779-017-0143-4 |
| _version_ | 1818488258672197632 |
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| author | Xian-Rong Shen Xiu-Li Chen Hai-Xia Xie Ying He Wei Chen Qun Luo Wei-Hong Yuan Xue Tang Deng-Yong Hou Ding-Wen Jiang Qing-Rong Wang |
| author_facet | Xian-Rong Shen Xiu-Li Chen Hai-Xia Xie Ying He Wei Chen Qun Luo Wei-Hong Yuan Xue Tang Deng-Yong Hou Ding-Wen Jiang Qing-Rong Wang |
| author_sort | Xian-Rong Shen |
| collection | DOAJ |
| description | Abstract Background Wounded personnel who work at sea often encounter a plethora of difficulties. The most important of these difficulties is seawater immersion. Common medical dressings have little effect when the affected area is immersed in seawater, and only rarely dressings have been reported for the treatment of seawater-immersed wounds. The objective of this study is to develop a new dressing which should be suitable to prevent the wound from seawater immersion and to promote the wound healing. Methods Shark skin collagen (SSC) was purified via ethanol de-sugaring and de-pigmentation and adjusted for pH. A shark skin collagen sponge (SSCS) was prepared by freeze-drying. SSCS was attached to an anti-seawater immersion polyurethane (PU) film (SSCS + PU) to compose a new dressing. The biochemical properties of SSC and physicochemical properties of SSCS were assessed by standard methods. The effects of SSCS and SSCS + PU on the healing of seawater-immersed wounds were studied using a seawater immersion rat model. For the detection of SSCS effects on seawater-immersed wounds, 12 SD rats, with four wounds created in each rat, were divided into four groups: the 3rd day group, 5th day group, 7th day group and 12th day group. In each group, six wounds were treated with SSCS, three wounds treated with chitosan served as the positive control, and three wounds treated with gauze served as the negative control. For the detection of the SSCS + PU effects on seawater-immersed wounds, 36 SD rats were divided into three groups: the gauze (GZ) + PU group, chitosan (CS) + PU group and SSCS + PU group, with 12 rats in each group, and two wounds in each rat. The wound sizes were measured to calculate the healing rate, and histomorphology and the immunohistochemistry of the CD31 and TGF-β expression levels in the wounded tissues were measured by standard methods. Results The results of Ultraviolet-visible (UV-vis) spectrum, Fourier-transform infrared (FTIR) spectrum, circular dichroism (CD) spectra, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and amino acid composition analyses of SSC demonstrated that SSC is type I collagen. SSCS had a homogeneous porous structure of approximately 200 μm, porosity rate of 83.57% ± 2.64%, water vapor transmission ratio (WVTR) of 4500 g/m2, tensile strength of 1.79 ± 0.41 N/mm, and elongation at break of 4.52% ± 0.01%. SSCS had significant beneficial effects on seawater-immersed wound healing. On the 3rd day, the healing rates in the GZ negative control, CS positive control and SSCS rats were 13.94% ± 5.50%, 29.40% ± 1.10% and 47.24% ± 8.40%, respectively. SSCS also enhanced TGF-β and CD31 expression in the initial stage of the healing period. The SSCS + PU dressing effectively protected wounds from seawater immersion for at least 4 h, and accelerated re-epithelialization, vascularization and granulation formation of seawater-immersed wounds in the earlier stages of wound healing, and as well as significantly promoted wound healing. The SSCS + PU dressing also enhanced expression of TGF-β and CD31. The effects of SSCS and SSCS + PU were superior to those of both the chitosan and gauze dressings. Conclusions SSCS has significant positive effects on the promotion of seawater-immersed wound healing, and a SSCS + PU dressing effectively prevents seawater immersion, and significantly promotes seawater-immersed wound healing. |
| first_indexed | 2024-12-10T16:48:38Z |
| format | Article |
| id | doaj.art-c7c97fb0f435492f9b7b6130bef39198 |
| institution | Directory Open Access Journal |
| issn | 2054-9369 |
| language | English |
| last_indexed | 2024-12-10T16:48:38Z |
| publishDate | 2017-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Military Medical Research |
| spelling | doaj.art-c7c97fb0f435492f9b7b6130bef391982022-12-22T01:40:58ZengBMCMilitary Medical Research2054-93692017-10-014111210.1186/s40779-017-0143-4Beneficial effects of a novel shark-skin collagen dressing for the promotion of seawater immersion wound healingXian-Rong Shen0Xiu-Li Chen1Hai-Xia Xie2Ying He3Wei Chen4Qun Luo5Wei-Hong Yuan6Xue Tang7Deng-Yong Hou8Ding-Wen Jiang9Qing-Rong Wang10The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research InstituteThe PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research InstituteThe PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research InstituteThe PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research InstituteThe PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research InstituteThe PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research InstituteThe PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research InstituteThe PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research InstituteThe PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research InstituteThe PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research InstituteThe PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research InstituteAbstract Background Wounded personnel who work at sea often encounter a plethora of difficulties. The most important of these difficulties is seawater immersion. Common medical dressings have little effect when the affected area is immersed in seawater, and only rarely dressings have been reported for the treatment of seawater-immersed wounds. The objective of this study is to develop a new dressing which should be suitable to prevent the wound from seawater immersion and to promote the wound healing. Methods Shark skin collagen (SSC) was purified via ethanol de-sugaring and de-pigmentation and adjusted for pH. A shark skin collagen sponge (SSCS) was prepared by freeze-drying. SSCS was attached to an anti-seawater immersion polyurethane (PU) film (SSCS + PU) to compose a new dressing. The biochemical properties of SSC and physicochemical properties of SSCS were assessed by standard methods. The effects of SSCS and SSCS + PU on the healing of seawater-immersed wounds were studied using a seawater immersion rat model. For the detection of SSCS effects on seawater-immersed wounds, 12 SD rats, with four wounds created in each rat, were divided into four groups: the 3rd day group, 5th day group, 7th day group and 12th day group. In each group, six wounds were treated with SSCS, three wounds treated with chitosan served as the positive control, and three wounds treated with gauze served as the negative control. For the detection of the SSCS + PU effects on seawater-immersed wounds, 36 SD rats were divided into three groups: the gauze (GZ) + PU group, chitosan (CS) + PU group and SSCS + PU group, with 12 rats in each group, and two wounds in each rat. The wound sizes were measured to calculate the healing rate, and histomorphology and the immunohistochemistry of the CD31 and TGF-β expression levels in the wounded tissues were measured by standard methods. Results The results of Ultraviolet-visible (UV-vis) spectrum, Fourier-transform infrared (FTIR) spectrum, circular dichroism (CD) spectra, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and amino acid composition analyses of SSC demonstrated that SSC is type I collagen. SSCS had a homogeneous porous structure of approximately 200 μm, porosity rate of 83.57% ± 2.64%, water vapor transmission ratio (WVTR) of 4500 g/m2, tensile strength of 1.79 ± 0.41 N/mm, and elongation at break of 4.52% ± 0.01%. SSCS had significant beneficial effects on seawater-immersed wound healing. On the 3rd day, the healing rates in the GZ negative control, CS positive control and SSCS rats were 13.94% ± 5.50%, 29.40% ± 1.10% and 47.24% ± 8.40%, respectively. SSCS also enhanced TGF-β and CD31 expression in the initial stage of the healing period. The SSCS + PU dressing effectively protected wounds from seawater immersion for at least 4 h, and accelerated re-epithelialization, vascularization and granulation formation of seawater-immersed wounds in the earlier stages of wound healing, and as well as significantly promoted wound healing. The SSCS + PU dressing also enhanced expression of TGF-β and CD31. The effects of SSCS and SSCS + PU were superior to those of both the chitosan and gauze dressings. Conclusions SSCS has significant positive effects on the promotion of seawater-immersed wound healing, and a SSCS + PU dressing effectively prevents seawater immersion, and significantly promotes seawater-immersed wound healing.http://link.springer.com/article/10.1186/s40779-017-0143-4Anti-seawater immersion dressingShark skin collagenSeawater immersion woundWound healing |
| spellingShingle | Xian-Rong Shen Xiu-Li Chen Hai-Xia Xie Ying He Wei Chen Qun Luo Wei-Hong Yuan Xue Tang Deng-Yong Hou Ding-Wen Jiang Qing-Rong Wang Beneficial effects of a novel shark-skin collagen dressing for the promotion of seawater immersion wound healing Military Medical Research Anti-seawater immersion dressing Shark skin collagen Seawater immersion wound Wound healing |
| title | Beneficial effects of a novel shark-skin collagen dressing for the promotion of seawater immersion wound healing |
| title_full | Beneficial effects of a novel shark-skin collagen dressing for the promotion of seawater immersion wound healing |
| title_fullStr | Beneficial effects of a novel shark-skin collagen dressing for the promotion of seawater immersion wound healing |
| title_full_unstemmed | Beneficial effects of a novel shark-skin collagen dressing for the promotion of seawater immersion wound healing |
| title_short | Beneficial effects of a novel shark-skin collagen dressing for the promotion of seawater immersion wound healing |
| title_sort | beneficial effects of a novel shark skin collagen dressing for the promotion of seawater immersion wound healing |
| topic | Anti-seawater immersion dressing Shark skin collagen Seawater immersion wound Wound healing |
| url | http://link.springer.com/article/10.1186/s40779-017-0143-4 |
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