iDISCO+ for the Study of Neuroimmune Architecture of the Rat Auditory Brainstem
The lower stations of the auditory system display a complex anatomy. The inner ear labyrinth is composed of several interconnecting membranous structures encased in cavities of the temporal bone, and the cerebellopontine angle contains fragile structures such as meningeal folds, the choroid plexus (...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Neuroanatomy |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnana.2019.00015/full |
| _version_ | 1818257166311620608 |
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| author | Paola Perin Fabian F. Voigt Fabian F. Voigt Philipp Bethge Philipp Bethge Fritjof Helmchen Fritjof Helmchen Roberto Pizzala |
| author_facet | Paola Perin Fabian F. Voigt Fabian F. Voigt Philipp Bethge Philipp Bethge Fritjof Helmchen Fritjof Helmchen Roberto Pizzala |
| author_sort | Paola Perin |
| collection | DOAJ |
| description | The lower stations of the auditory system display a complex anatomy. The inner ear labyrinth is composed of several interconnecting membranous structures encased in cavities of the temporal bone, and the cerebellopontine angle contains fragile structures such as meningeal folds, the choroid plexus (CP), and highly variable vascular formations. For this reason, most histological studies of the auditory system have either focused on the inner ear or the CNS by physically detaching the temporal bone from the brainstem. However, several studies of neuroimmune interactions have pinpointed the importance of structures such as meninges and CP; in the auditory system, an immune function has also been suggested for inner ear structures such as the endolymphatic duct (ED) and sac. All these structures are thin, fragile, and have complex 3D shapes. In order to study the immune cell populations located on these structures and their relevance to the inner ear and auditory brainstem in health and disease, we obtained a clarified-decalcified preparation of the rat hindbrain still attached to the intact temporal bone. This preparation may be immunolabeled using a clearing protocol (based on iDISCO+) to show location and functional state of immune cells. The observed macrophage distribution suggests the presence of CP-mediated communication pathways between the inner ear and the cochlear nuclei. |
| first_indexed | 2024-12-12T17:39:20Z |
| format | Article |
| id | doaj.art-c7cb97f6e66c4cbfb20037acf35de27a |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-12-12T17:39:20Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-c7cb97f6e66c4cbfb20037acf35de27a2022-12-22T00:17:08ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292019-02-011310.3389/fnana.2019.00015440265iDISCO+ for the Study of Neuroimmune Architecture of the Rat Auditory BrainstemPaola Perin0Fabian F. Voigt1Fabian F. Voigt2Philipp Bethge3Philipp Bethge4Fritjof Helmchen5Fritjof Helmchen6Roberto Pizzala7Department of Brain and Behavioural Sciences, University of Pavia, Pavia, ItalyBrain Research Institute, University of Zurich, Zürich, SwitzerlandCenter for Neurosciences, University of Zurich and ETH Zurich, Zürich, SwitzerlandBrain Research Institute, University of Zurich, Zürich, SwitzerlandCenter for Neurosciences, University of Zurich and ETH Zurich, Zürich, SwitzerlandBrain Research Institute, University of Zurich, Zürich, SwitzerlandCenter for Neurosciences, University of Zurich and ETH Zurich, Zürich, SwitzerlandDepartment of Molecular Medicine, University of Pavia, Pavia, ItalyThe lower stations of the auditory system display a complex anatomy. The inner ear labyrinth is composed of several interconnecting membranous structures encased in cavities of the temporal bone, and the cerebellopontine angle contains fragile structures such as meningeal folds, the choroid plexus (CP), and highly variable vascular formations. For this reason, most histological studies of the auditory system have either focused on the inner ear or the CNS by physically detaching the temporal bone from the brainstem. However, several studies of neuroimmune interactions have pinpointed the importance of structures such as meninges and CP; in the auditory system, an immune function has also been suggested for inner ear structures such as the endolymphatic duct (ED) and sac. All these structures are thin, fragile, and have complex 3D shapes. In order to study the immune cell populations located on these structures and their relevance to the inner ear and auditory brainstem in health and disease, we obtained a clarified-decalcified preparation of the rat hindbrain still attached to the intact temporal bone. This preparation may be immunolabeled using a clearing protocol (based on iDISCO+) to show location and functional state of immune cells. The observed macrophage distribution suggests the presence of CP-mediated communication pathways between the inner ear and the cochlear nuclei.https://www.frontiersin.org/article/10.3389/fnana.2019.00015/fullchoroid plexuscochlear nucleusinner earmicrogliamacrophageauditory system |
| spellingShingle | Paola Perin Fabian F. Voigt Fabian F. Voigt Philipp Bethge Philipp Bethge Fritjof Helmchen Fritjof Helmchen Roberto Pizzala iDISCO+ for the Study of Neuroimmune Architecture of the Rat Auditory Brainstem Frontiers in Neuroanatomy choroid plexus cochlear nucleus inner ear microglia macrophage auditory system |
| title | iDISCO+ for the Study of Neuroimmune Architecture of the Rat Auditory Brainstem |
| title_full | iDISCO+ for the Study of Neuroimmune Architecture of the Rat Auditory Brainstem |
| title_fullStr | iDISCO+ for the Study of Neuroimmune Architecture of the Rat Auditory Brainstem |
| title_full_unstemmed | iDISCO+ for the Study of Neuroimmune Architecture of the Rat Auditory Brainstem |
| title_short | iDISCO+ for the Study of Neuroimmune Architecture of the Rat Auditory Brainstem |
| title_sort | idisco for the study of neuroimmune architecture of the rat auditory brainstem |
| topic | choroid plexus cochlear nucleus inner ear microglia macrophage auditory system |
| url | https://www.frontiersin.org/article/10.3389/fnana.2019.00015/full |
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