Industrial-Scale Production of Mycotoxin Binder from the Red Yeast <i>Sporidiobolus pararoseus</i> KM281507
Red yeast <i>Sporidiobolus pararoseus</i> KM281507 has been recognized as a potential feed additive. Beyond their nutritional value (carotenoids and lipids), red yeast cells (RYCs) containing high levels of β-glucan can bind mycotoxins. This study investigated the industrial feasibility...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/4/353 |
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| author | Wanaporn Tapingkae Orranee Srinual Chompunut Lumsangkul Hien Van Doan Hsin-I Chiang Atchara Manowattana Pinpanit Boonchuay Thanongsak Chaiyaso |
| author_facet | Wanaporn Tapingkae Orranee Srinual Chompunut Lumsangkul Hien Van Doan Hsin-I Chiang Atchara Manowattana Pinpanit Boonchuay Thanongsak Chaiyaso |
| author_sort | Wanaporn Tapingkae |
| collection | DOAJ |
| description | Red yeast <i>Sporidiobolus pararoseus</i> KM281507 has been recognized as a potential feed additive. Beyond their nutritional value (carotenoids and lipids), red yeast cells (RYCs) containing high levels of β-glucan can bind mycotoxins. This study investigated the industrial feasibility of the large-scale production of RYCs, along with their ability to act as a mycotoxin binder. Under a semi-controlled pH condition in a 300 L bioreactor, 28.70-g/L biomass, 8.67-g/L lipids, and 96.10-mg/L total carotenoids were obtained, and the RYCs were found to contain 5.73% (<i>w</i>/<i>w</i>) β-glucan. The encapsulated RYC was in vitro tested for its mycotoxin adsorption capacity, including for aflatoxin B1 (AFB1), zearalenone (ZEA), ochratoxin A (OTA), T-2 toxin (T-2) and deoxynivalenol (DON). The RYCs had the highest binding capacity for OTA and T-2 at concentrations of 0.31–1.25 and 0.31–2.5 µg/mL, respectively. The mycotoxin adsorption capacity was further tested using a gastrointestinal poultry model. The adsorption capacities of the RYCs and a commercial mycotoxin binder (CMB) were comparable. The RYCs not only are rich in lipids and carotenoids but also play an important role in mycotoxin binding. Since the industrial-scale production and downstream processing of RYCs were successfully demonstrated, RYCs could be applied as possible feed additives. |
| first_indexed | 2024-03-09T13:27:37Z |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T13:27:37Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-c7cd3da7e49443c3b4ced51c0e63027b2023-11-30T21:21:54ZengMDPI AGJournal of Fungi2309-608X2022-03-018435310.3390/jof8040353Industrial-Scale Production of Mycotoxin Binder from the Red Yeast <i>Sporidiobolus pararoseus</i> KM281507Wanaporn Tapingkae0Orranee Srinual1Chompunut Lumsangkul2Hien Van Doan3Hsin-I Chiang4Atchara Manowattana5Pinpanit Boonchuay6Thanongsak Chaiyaso7Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Animal Science, National Chung Hsing University, Taichung 40227, TaiwanFaculty of Arts and Sciences, Western University, Kanchanaburi 71170, ThailandDivision of Biotechnology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandDivision of Biotechnology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandRed yeast <i>Sporidiobolus pararoseus</i> KM281507 has been recognized as a potential feed additive. Beyond their nutritional value (carotenoids and lipids), red yeast cells (RYCs) containing high levels of β-glucan can bind mycotoxins. This study investigated the industrial feasibility of the large-scale production of RYCs, along with their ability to act as a mycotoxin binder. Under a semi-controlled pH condition in a 300 L bioreactor, 28.70-g/L biomass, 8.67-g/L lipids, and 96.10-mg/L total carotenoids were obtained, and the RYCs were found to contain 5.73% (<i>w</i>/<i>w</i>) β-glucan. The encapsulated RYC was in vitro tested for its mycotoxin adsorption capacity, including for aflatoxin B1 (AFB1), zearalenone (ZEA), ochratoxin A (OTA), T-2 toxin (T-2) and deoxynivalenol (DON). The RYCs had the highest binding capacity for OTA and T-2 at concentrations of 0.31–1.25 and 0.31–2.5 µg/mL, respectively. The mycotoxin adsorption capacity was further tested using a gastrointestinal poultry model. The adsorption capacities of the RYCs and a commercial mycotoxin binder (CMB) were comparable. The RYCs not only are rich in lipids and carotenoids but also play an important role in mycotoxin binding. Since the industrial-scale production and downstream processing of RYCs were successfully demonstrated, RYCs could be applied as possible feed additives.https://www.mdpi.com/2309-608X/8/4/353300 L bioreactoradsorbentsβ-glucanfeed additivegastrointestinal model |
| spellingShingle | Wanaporn Tapingkae Orranee Srinual Chompunut Lumsangkul Hien Van Doan Hsin-I Chiang Atchara Manowattana Pinpanit Boonchuay Thanongsak Chaiyaso Industrial-Scale Production of Mycotoxin Binder from the Red Yeast <i>Sporidiobolus pararoseus</i> KM281507 Journal of Fungi 300 L bioreactor adsorbents β-glucan feed additive gastrointestinal model |
| title | Industrial-Scale Production of Mycotoxin Binder from the Red Yeast <i>Sporidiobolus pararoseus</i> KM281507 |
| title_full | Industrial-Scale Production of Mycotoxin Binder from the Red Yeast <i>Sporidiobolus pararoseus</i> KM281507 |
| title_fullStr | Industrial-Scale Production of Mycotoxin Binder from the Red Yeast <i>Sporidiobolus pararoseus</i> KM281507 |
| title_full_unstemmed | Industrial-Scale Production of Mycotoxin Binder from the Red Yeast <i>Sporidiobolus pararoseus</i> KM281507 |
| title_short | Industrial-Scale Production of Mycotoxin Binder from the Red Yeast <i>Sporidiobolus pararoseus</i> KM281507 |
| title_sort | industrial scale production of mycotoxin binder from the red yeast i sporidiobolus pararoseus i km281507 |
| topic | 300 L bioreactor adsorbents β-glucan feed additive gastrointestinal model |
| url | https://www.mdpi.com/2309-608X/8/4/353 |
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