Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex
Subplate neurons (SPNs) are thought to play a role in nascent sensory processing in neocortex. To better understand how heterogeneity within this population relates to emergent function, we investigated the synaptic connectivity of Lpar1-EGFP SPNs through the first postnatal week in whisker somatose...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-07-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/60810 |
| _version_ | 1818026733603913728 |
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| author | Filippo Ghezzi Andre Marques-Smith Paul G Anastasiades Daniel Lyngholm Cristiana Vagnoni Alexandra Rowett Gokul Parameswaran Anna Hoerder-Suabedissen Yasushi Nakagawa Zoltan Molnar Simon JB Butt |
| author_facet | Filippo Ghezzi Andre Marques-Smith Paul G Anastasiades Daniel Lyngholm Cristiana Vagnoni Alexandra Rowett Gokul Parameswaran Anna Hoerder-Suabedissen Yasushi Nakagawa Zoltan Molnar Simon JB Butt |
| author_sort | Filippo Ghezzi |
| collection | DOAJ |
| description | Subplate neurons (SPNs) are thought to play a role in nascent sensory processing in neocortex. To better understand how heterogeneity within this population relates to emergent function, we investigated the synaptic connectivity of Lpar1-EGFP SPNs through the first postnatal week in whisker somatosensory cortex (S1BF). These SPNs comprise of two morphological subtypes: fusiform SPNs with local axons and pyramidal SPNs with axons that extend through the marginal zone. The former receive translaminar synaptic input up until the emergence of the whisker barrels, a timepoint coincident with significant cell death. In contrast, pyramidal SPNs receive local input from the subplate at early ages but then – during the later time window – acquire input from overlying cortex. Combined electrical and optogenetic activation of thalamic afferents identified that Lpar1-EGFP SPNs receive sparse thalamic innervation. These data reveal components of the postnatal network that interpret sparse thalamic input to direct the emergent columnar structure of S1BF. |
| first_indexed | 2024-12-10T04:36:42Z |
| format | Article |
| id | doaj.art-c7d3e2030c9a4028b225ee22163067be |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T04:36:42Z |
| publishDate | 2021-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c7d3e2030c9a4028b225ee22163067be2022-12-22T02:02:00ZengeLife Sciences Publications LtdeLife2050-084X2021-07-011010.7554/eLife.60810Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortexFilippo Ghezzi0https://orcid.org/0000-0003-4538-2578Andre Marques-Smith1https://orcid.org/0000-0001-6879-2858Paul G Anastasiades2Daniel Lyngholm3https://orcid.org/0000-0002-3708-0249Cristiana Vagnoni4Alexandra Rowett5Gokul Parameswaran6Anna Hoerder-Suabedissen7https://orcid.org/0000-0003-1953-7871Yasushi Nakagawa8https://orcid.org/0000-0003-4876-5718Zoltan Molnar9https://orcid.org/0000-0002-6852-6004Simon JB Butt10https://orcid.org/0000-0002-2399-0102Department of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Oxford, United KingdomDepartment of Neuroscience, University of Minnesota, Minneapolis, United StatesDepartment of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Oxford, United KingdomSubplate neurons (SPNs) are thought to play a role in nascent sensory processing in neocortex. To better understand how heterogeneity within this population relates to emergent function, we investigated the synaptic connectivity of Lpar1-EGFP SPNs through the first postnatal week in whisker somatosensory cortex (S1BF). These SPNs comprise of two morphological subtypes: fusiform SPNs with local axons and pyramidal SPNs with axons that extend through the marginal zone. The former receive translaminar synaptic input up until the emergence of the whisker barrels, a timepoint coincident with significant cell death. In contrast, pyramidal SPNs receive local input from the subplate at early ages but then – during the later time window – acquire input from overlying cortex. Combined electrical and optogenetic activation of thalamic afferents identified that Lpar1-EGFP SPNs receive sparse thalamic innervation. These data reveal components of the postnatal network that interpret sparse thalamic input to direct the emergent columnar structure of S1BF.https://elifesciences.org/articles/60810neocortexneural developmentsubplateneural circuitsoptogeneticslaser scanning photostimulation |
| spellingShingle | Filippo Ghezzi Andre Marques-Smith Paul G Anastasiades Daniel Lyngholm Cristiana Vagnoni Alexandra Rowett Gokul Parameswaran Anna Hoerder-Suabedissen Yasushi Nakagawa Zoltan Molnar Simon JB Butt Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex eLife neocortex neural development subplate neural circuits optogenetics laser scanning photostimulation |
| title | Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex |
| title_full | Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex |
| title_fullStr | Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex |
| title_full_unstemmed | Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex |
| title_short | Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex |
| title_sort | non canonical role for lpar1 egfp subplate neurons in early postnatal mouse somatosensory cortex |
| topic | neocortex neural development subplate neural circuits optogenetics laser scanning photostimulation |
| url | https://elifesciences.org/articles/60810 |
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