Parameter Reliability and Understanding Enzyme Function
Knowledge of the Michaelis–Menten parameters and their meaning in different circumstances is an essential prerequisite to understanding enzyme function and behaviour. The published literature contains an abundance of values reported for many enzymes. The problem concerns assessing the appropriatenes...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/1/263 |
| _version_ | 1797498215921090560 |
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| author | Andrew G. McDonald Keith F. Tipton |
| author_facet | Andrew G. McDonald Keith F. Tipton |
| author_sort | Andrew G. McDonald |
| collection | DOAJ |
| description | Knowledge of the Michaelis–Menten parameters and their meaning in different circumstances is an essential prerequisite to understanding enzyme function and behaviour. The published literature contains an abundance of values reported for many enzymes. The problem concerns assessing the appropriateness and validity of such material for the purpose to which it is to be applied. This review considers the evaluation of such data with particular emphasis on the assessment of its fitness for purpose. |
| first_indexed | 2024-03-10T03:30:15Z |
| format | Article |
| id | doaj.art-c7d3ece2373c40ec87c24aa8feca5595 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:30:15Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c7d3ece2373c40ec87c24aa8feca55952023-11-23T11:59:05ZengMDPI AGMolecules1420-30492022-01-0127126310.3390/molecules27010263Parameter Reliability and Understanding Enzyme FunctionAndrew G. McDonald0Keith F. Tipton1School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, D02 PN40 Dublin, IrelandSchool of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, D02 PN40 Dublin, IrelandKnowledge of the Michaelis–Menten parameters and their meaning in different circumstances is an essential prerequisite to understanding enzyme function and behaviour. The published literature contains an abundance of values reported for many enzymes. The problem concerns assessing the appropriateness and validity of such material for the purpose to which it is to be applied. This review considers the evaluation of such data with particular emphasis on the assessment of its fitness for purpose.https://www.mdpi.com/1420-3049/27/1/263enzyme kineticsMichaelis constantmaximal velocitysubstrate competitionenzyme inhibitioncooperativity |
| spellingShingle | Andrew G. McDonald Keith F. Tipton Parameter Reliability and Understanding Enzyme Function Molecules enzyme kinetics Michaelis constant maximal velocity substrate competition enzyme inhibition cooperativity |
| title | Parameter Reliability and Understanding Enzyme Function |
| title_full | Parameter Reliability and Understanding Enzyme Function |
| title_fullStr | Parameter Reliability and Understanding Enzyme Function |
| title_full_unstemmed | Parameter Reliability and Understanding Enzyme Function |
| title_short | Parameter Reliability and Understanding Enzyme Function |
| title_sort | parameter reliability and understanding enzyme function |
| topic | enzyme kinetics Michaelis constant maximal velocity substrate competition enzyme inhibition cooperativity |
| url | https://www.mdpi.com/1420-3049/27/1/263 |
| work_keys_str_mv | AT andrewgmcdonald parameterreliabilityandunderstandingenzymefunction AT keithftipton parameterreliabilityandunderstandingenzymefunction |