Antioxidant and Hypoglycemic Activity of Sacha Inchi Meal Protein Hydrolysate
Sacha inchi meal (SIM) is the residue from the processing of the oil crop sacha inchi. In the present study, biological enzymolysis was performed on SIM protein to obtain hydrolysates, and then the antioxidant and hypoglycemic activities were evaluated. The results showed that the scavenging rates o...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/11/6528 |
| _version_ | 1797597894587449344 |
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| author | Tianyu Shu Kai Wang Xuewu Zhang |
| author_facet | Tianyu Shu Kai Wang Xuewu Zhang |
| author_sort | Tianyu Shu |
| collection | DOAJ |
| description | Sacha inchi meal (SIM) is the residue from the processing of the oil crop sacha inchi. In the present study, biological enzymolysis was performed on SIM protein to obtain hydrolysates, and then the antioxidant and hypoglycemic activities were evaluated. The results showed that the scavenging rates of alkaline protease hydrolysate (SAl) and protamex hydrolysate (SPr) to ABTS free radicals were close to that of vitamin C at 5 mg/mL, amounting to 99.83 ± 0.33% and 100.00 ± 0.09%, respectively. The dipeptidyl peptidase (DPP-IV) inhibitory activities of SPr and SAl were also the highest, at the concentration of 2.5 mg/mL, their inhibition rates were 74.15 ± 0.68% and 56.38 ± 1.51%, respectively, with the IC<sub>50</sub> values of 1.007 mg/mL and 2.130 mg/mL, respectively. Moreover, compared with the model group, all the hydrolysates increased the glucose consumption by 187.01–348.79% (at 800 μg/mL) in insulin-resistant HepG2 cells, which were better than positive drug Metformin. In conclusion, SIM protein hydrolysates have significant antioxidant and hypoglycemic activity in vitro; therefore, the hydrolysates could be used as for functional food and pharmaceutical development. |
| first_indexed | 2024-03-11T03:11:50Z |
| format | Article |
| id | doaj.art-c7d619c01f6940e9a816bf937d79e89d |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T03:11:50Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-c7d619c01f6940e9a816bf937d79e89d2023-11-18T07:33:20ZengMDPI AGApplied Sciences2076-34172023-05-011311652810.3390/app13116528Antioxidant and Hypoglycemic Activity of Sacha Inchi Meal Protein HydrolysateTianyu Shu0Kai Wang1Xuewu Zhang2College of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaCollege of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaCollege of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaSacha inchi meal (SIM) is the residue from the processing of the oil crop sacha inchi. In the present study, biological enzymolysis was performed on SIM protein to obtain hydrolysates, and then the antioxidant and hypoglycemic activities were evaluated. The results showed that the scavenging rates of alkaline protease hydrolysate (SAl) and protamex hydrolysate (SPr) to ABTS free radicals were close to that of vitamin C at 5 mg/mL, amounting to 99.83 ± 0.33% and 100.00 ± 0.09%, respectively. The dipeptidyl peptidase (DPP-IV) inhibitory activities of SPr and SAl were also the highest, at the concentration of 2.5 mg/mL, their inhibition rates were 74.15 ± 0.68% and 56.38 ± 1.51%, respectively, with the IC<sub>50</sub> values of 1.007 mg/mL and 2.130 mg/mL, respectively. Moreover, compared with the model group, all the hydrolysates increased the glucose consumption by 187.01–348.79% (at 800 μg/mL) in insulin-resistant HepG2 cells, which were better than positive drug Metformin. In conclusion, SIM protein hydrolysates have significant antioxidant and hypoglycemic activity in vitro; therefore, the hydrolysates could be used as for functional food and pharmaceutical development.https://www.mdpi.com/2076-3417/13/11/6528by-producthydrolysisDPP-IVglucose consumption |
| spellingShingle | Tianyu Shu Kai Wang Xuewu Zhang Antioxidant and Hypoglycemic Activity of Sacha Inchi Meal Protein Hydrolysate Applied Sciences by-product hydrolysis DPP-IV glucose consumption |
| title | Antioxidant and Hypoglycemic Activity of Sacha Inchi Meal Protein Hydrolysate |
| title_full | Antioxidant and Hypoglycemic Activity of Sacha Inchi Meal Protein Hydrolysate |
| title_fullStr | Antioxidant and Hypoglycemic Activity of Sacha Inchi Meal Protein Hydrolysate |
| title_full_unstemmed | Antioxidant and Hypoglycemic Activity of Sacha Inchi Meal Protein Hydrolysate |
| title_short | Antioxidant and Hypoglycemic Activity of Sacha Inchi Meal Protein Hydrolysate |
| title_sort | antioxidant and hypoglycemic activity of sacha inchi meal protein hydrolysate |
| topic | by-product hydrolysis DPP-IV glucose consumption |
| url | https://www.mdpi.com/2076-3417/13/11/6528 |
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