Detection and genetic variability of newly identified dasheen mosaic virus in Poland
Dasheen mosaic virus (DsMV) is one of the most important viral pathogens of aroids and can cause major economic losses for ornamental crops. Here, we present the detection and molecular characterisation of DsMV isolates originating from Monstera adansonii plants in Poland. Moreover, the genetic vari...
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| Format: | Article |
| Language: | English |
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Polish Academy of Sciences
2022-10-01
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| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://journals.pan.pl/Content/124812/PDF/OA_01_JPPR_62_4_1399_Taberska.pdf |
| _version_ | 1797951681707638784 |
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| author | Agnieszka Taberska Julia Minicka Daria Budzyńska Beata Hasiów-Jaroszewska |
| author_facet | Agnieszka Taberska Julia Minicka Daria Budzyńska Beata Hasiów-Jaroszewska |
| author_sort | Agnieszka Taberska |
| collection | DOAJ |
| description | Dasheen mosaic virus (DsMV) is one of the most important viral pathogens of aroids and can cause major economic losses for ornamental crops. Here, we present the detection and molecular characterisation of DsMV isolates originating from Monstera adansonii plants in Poland. Moreover, the genetic variability of DsMV isolates was analyzed based on the coat protein gene ( CP) of the Polish and other DsMV isolates described to date. The presence of DsMV was confirmed by transmission electron microscopy (TEM) and reverse transcription polymerase chain reaction (RT-PCR) with specific, diagnostic primers in three out of ten examined plants. To obtain full-length sequences of CP, two pairs of primers were designed and used in the RT-PCR. The specificity of obtained products was confirmed by Sanger sequencing. The obtained sequences of CP were compared with 44 other DsMV sequences retrieved from the GenBank. Analyses revealed that DsMV population is very diverse. The variability of DsMV isolates was confirmed by low sequence identity and pervasive recombination events. The phylogenetic analysis was performed based on 37 non-recombinant CP sequences. The maximum-likelihood reconstruction revealed that the Polish isolates are distinct and grouped separately from other DsMV isolates. Due to the high genetic diversity, detecting the virus could be difficult. Nonetheless disease management relies strongly on a fast and accurate identification of the causal agent. To our knowledge this is the first report of DsMV in Poland. |
| first_indexed | 2024-04-10T22:34:25Z |
| format | Article |
| id | doaj.art-c7d76e83183c4209a6bd52ce257c670e |
| institution | Directory Open Access Journal |
| issn | 1427-4345 |
| language | English |
| last_indexed | 2024-04-10T22:34:25Z |
| publishDate | 2022-10-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Journal of Plant Protection Research |
| spelling | doaj.art-c7d76e83183c4209a6bd52ce257c670e2023-01-16T14:50:04ZengPolish Academy of SciencesJournal of Plant Protection Research1427-43452022-10-01vol. 62No 4307316https://doi.org/10.24425/jppr.2022.140301Detection and genetic variability of newly identified dasheen mosaic virus in PolandAgnieszka Taberska0Julia Minicka1Daria Budzyńska2Beata Hasiów-Jaroszewska3https://orcid.org/0000-0002-8267-023XDepartment of Virology and Bacteriology, Institute of Plant Protection – National Research Institute, Poznań, PolandDepartment of Virology and Bacteriology, Institute of Plant Protection – National Research Institute, Poznań, PolandDepartment of Virology and Bacteriology, Institute of Plant Protection – National Research Institute, Poznań, PolandDepartment of Virology and Bacteriology, Institute of Plant Protection – National Research Institute, Poznań, PolandDasheen mosaic virus (DsMV) is one of the most important viral pathogens of aroids and can cause major economic losses for ornamental crops. Here, we present the detection and molecular characterisation of DsMV isolates originating from Monstera adansonii plants in Poland. Moreover, the genetic variability of DsMV isolates was analyzed based on the coat protein gene ( CP) of the Polish and other DsMV isolates described to date. The presence of DsMV was confirmed by transmission electron microscopy (TEM) and reverse transcription polymerase chain reaction (RT-PCR) with specific, diagnostic primers in three out of ten examined plants. To obtain full-length sequences of CP, two pairs of primers were designed and used in the RT-PCR. The specificity of obtained products was confirmed by Sanger sequencing. The obtained sequences of CP were compared with 44 other DsMV sequences retrieved from the GenBank. Analyses revealed that DsMV population is very diverse. The variability of DsMV isolates was confirmed by low sequence identity and pervasive recombination events. The phylogenetic analysis was performed based on 37 non-recombinant CP sequences. The maximum-likelihood reconstruction revealed that the Polish isolates are distinct and grouped separately from other DsMV isolates. Due to the high genetic diversity, detecting the virus could be difficult. Nonetheless disease management relies strongly on a fast and accurate identification of the causal agent. To our knowledge this is the first report of DsMV in Poland.https://journals.pan.pl/Content/124812/PDF/OA_01_JPPR_62_4_1399_Taberska.pdfdasheen mosaic virus (dsmv)genetic variabilitymonstera adansoniirt-pcr |
| spellingShingle | Agnieszka Taberska Julia Minicka Daria Budzyńska Beata Hasiów-Jaroszewska Detection and genetic variability of newly identified dasheen mosaic virus in Poland Journal of Plant Protection Research dasheen mosaic virus (dsmv) genetic variability monstera adansonii rt-pcr |
| title | Detection and genetic variability of newly identified dasheen mosaic virus in Poland |
| title_full | Detection and genetic variability of newly identified dasheen mosaic virus in Poland |
| title_fullStr | Detection and genetic variability of newly identified dasheen mosaic virus in Poland |
| title_full_unstemmed | Detection and genetic variability of newly identified dasheen mosaic virus in Poland |
| title_short | Detection and genetic variability of newly identified dasheen mosaic virus in Poland |
| title_sort | detection and genetic variability of newly identified dasheen mosaic virus in poland |
| topic | dasheen mosaic virus (dsmv) genetic variability monstera adansonii rt-pcr |
| url | https://journals.pan.pl/Content/124812/PDF/OA_01_JPPR_62_4_1399_Taberska.pdf |
| work_keys_str_mv | AT agnieszkataberska detectionandgeneticvariabilityofnewlyidentifieddasheenmosaicvirusinpoland AT juliaminicka detectionandgeneticvariabilityofnewlyidentifieddasheenmosaicvirusinpoland AT dariabudzynska detectionandgeneticvariabilityofnewlyidentifieddasheenmosaicvirusinpoland AT beatahasiowjaroszewska detectionandgeneticvariabilityofnewlyidentifieddasheenmosaicvirusinpoland |